- Fast genetic variation among coliphage quasispecies revealed by a random amplified polymorphic DNA (RAPD) analysis
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Kwon, Oh Sik , Lee, Jae Yung
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J. Microbiol. 1996;34(2):166-171.
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 Abstract
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Genetic analysis was conducted on newly isolated coliphages form soil by using a RAPD assay. From the initial result, the coliphages were turned out to be different form one another but were closely related to φλ due to the fact that they shared the same RAPD maker in which other T phage testing failed to show. By using the primers EC01 or EC02, a fast genetic mutation of φC1 was found by producing specific RAPD markers on the phages from the first filial progeny to the second filial progeny. When we made a RAPD assay with combined primers (EC01, EC05 and EC08), the genetic mutation was again confirmed in φC1. The assay detection showed mutations in other coliphages such as φC2 and φC3 by revealing specific RAPD bands among different progeny phages, where genetic instability of the coliphages in implied.
- Genetic Variation of BIV Isolates Characterized by PCR Using Degenerate Primers
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Kwon, Oh Sik , Sninsky, John J.
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J. Microbiol. 1995;33(3):252-259.
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Abstract
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The PCR was employed to detect and characterize the bovine immunodeficiency-like virus (BIV), which is a newly recognized member of the Lentivirinae of the retroviruses. Degenerate primers representing the conserved regions in the pol genes of the Lentivirinae, were used to detect proviral DNA obtained from the bovine embryonic spleen cell cultures infected with BIV. The PCR amplified DNA fragment was molecularly cloned and sequenced. The BIV DNA fragment contained a sequence identical to that reported by Garvey et al. (Garvey et al., 1990. Virology, 175, 391-409). With the degenerate primers, peripheral blood mononuclear cells (PBMCs) of sick cattle and cells cultured with BIV were tested to determine genetic variation of BIV pol conserved sequence. We found the sequence heterogeneity within cultures and most variations occurred at the third base of codons that would not lead to amino acid substitutions. Another change was GAG (Glu) to AAG (Lys) within the BIV isolates. Interestingly, the altered sequence is also found in other lentiviruses such as HIV-2, SIV mac, CAEV and EIAV.
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