- Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli
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Yusuke Yoshida , Shinichiro Sugiyama , Tomoya Oyamada , Katsushi Yokoyama , Soo-Ki Kim , Kozo Makino
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J. Microbiol. 2011;49(2):285-289. Published online May 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0360-6
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By using a lacZ operon fusion genomic library of the Escherichia coli O157:H7 Sakai, we identified phosphatestarvation-inducible (psi) promoters located upstream of the yibD and ytfK genes. They have been previously proposed to belong to the phosphate regulon (pho regulon) by Beak and Lee (2006), based on the DNA array and in vivo transcriptional experiments. However, the direct interaction of these promoters with the activator protein of the pho regulon, PhoB, has not been determined. We determined the binding regions of PhoB in these promoter regions by DNase I footprinting. Both regions contained two pho boxes similar to the consensus sequence for PhoB binding.
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Citations
Citations to this article as recorded by  - Engineering of bacteria towards programmed autolysis: why, how, and when?
Changying Dong, Shenghao Cui, Jialuan Ren, Guoli Gong, Jian Zha, Xia Wu
Microbial Cell Factories.2024;[Epub] CrossRef - Regulation of PhoB on biofilm formation and hemolysin gene hlyA and ciaR of Streptococcus agalactiae
Xiaohui Cai, Shaoyu Yang, Yinhui Peng, Kianann Tan, Peng Xu, Zaohe Wu, Kit Yue Kwan, Jichang Jian
Veterinary Microbiology.2024; 289: 109961. CrossRef - Functional expansion of the natural inorganic phosphorus starvation response system in Escherichia coli
Zheng Yu, Wenna Li, Chang Ge, Xinxiao Sun, Jia Wang, Xiaolin Shen, Qipeng Yuan
Biotechnology Advances.2023; 66: 108154. CrossRef - Genome-Wide Mapping of the Escherichia coli PhoB Regulon Reveals Many Transcriptionally Inert, Intragenic Binding Sites
Devon M. Fitzgerald, Anne M. Stringer, Carol Smith, Pascal Lapierre, Joseph T. Wade, Michael T. Laub
mBio.2023;[Epub] CrossRef - Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
Laura Meyer, Elsa Germain, Etienne Maisonneuve
Frontiers in Microbiology.2021;[Epub] CrossRef -
Regulation of
waaH
by PhoB during P
i
Starvation Promotes Biofilm Formation by Escherichia coli O157:H7
Philippe Vogeleer, Antony T. Vincent, Samuel M. Chekabab, Steve J. Charette, Alexey Novikov, Martine Caroff, Francis Beaudry, Mario Jacques, Josée Harel, George O'Toole
Journal of Bacteriology.2019;[Epub] CrossRef -
Control of the
phoBR
Regulon in
Escherichia coli
Stewart G. Gardner, William R. McCleary, James M. Slauch
EcoSal Plus.2019;[Epub] CrossRef - YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli
Elsa Germain, Paul Guiraud, Deborah Byrne, Badreddine Douzi, Meriem Djendli, Etienne Maisonneuve
Nature Communications.2019;[Epub] CrossRef - Involvement of the ytfK gene from the PhoB regulon in stationary-phase H 2 O 2 stress tolerance in Escherichia coli
Yumi Iwadate, Jun-ichi Kato
Microbiology.2017; 163(12): 1912. CrossRef - Molecular and Structural Basis of Inner Core Lipopolysaccharide Alterations in Escherichia coli
Gracjana Klein, Sven Müller-Loennies, Buko Lindner, Natalia Kobylak, Helmut Brade, Satish Raina
Journal of Biological Chemistry.2013; 288(12): 8111. CrossRef - Novel members of the phosphate regulon in Escherichia coli O157:H7 identified using a whole-genome shotgun approach
Yusuke Yoshida, Shinichiro Sugiyama, Tomoya Oyamada, Katsushi Yokoyama, Kozo Makino
Gene.2012; 502(1): 27. CrossRef
- Expression of Escherichia coli DcuS-R Two-Component Regulatory System is Regulated by the Secondary Internal Promoter Which is Activated by CRP-cAMP
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Tomoya Oyamada , Katsushi Yokoyama , Michiko Morinaga , Masashi Suzuki , Kozo Makino
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J. Microbiol. 2007;45(3):234-240.
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DOI: https://doi.org/2537 [pii]
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Abstract
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The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.
- Mechanism of Transcriptional Activation of the Phosphate Regulon in Escherichi coli
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Kozo Makino , Mitsuko Amemura , Soo-Ki Kim , Katsushi Yokoyama , Sigenobu Kimura
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J. Microbiol. 1998;36(4):231-238.
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Abstract
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In Escherichia coli, at least 31 genes, which are involved in the roles related to the transport and assimilation of phosphate and phosphorus compounds, are induced by phosphate starvation. They constitute a single phosphate (pho) regulon, and are under the same physiological and genetic control (30, 36, 46). Proteins PhoB and PhoR, which are regulatory systems for the transcriptional regulation of the phogenes, belong to a large family of two-component regulatory systems that respond to a variety of environmental stimuli in bacteria (23, 24, 33, 39). PhoB is the transcriptional activator, which binds to the promoters of the pho genes (21, 22). PhoR is a transmembrane protein that modulates the activity of PhoB by promoting specific phosphorylation and dephosphorylation of PhoB in response to the phosphate signal in the medium (19, 21, 37, 50). The phosphorylation of PhoB protein occurs concurrently with the acquisition of the ability to activate transcription from the pho promoters (Fig. 1). In the absence of the PhoR functions, PhoB is phosphorylated independently of the phosphate levels by PhoM, a PhoR like protein (2, 3, 26), which was renamed CreC by Wanner (45). In this article, we describe our recent studies on the mechanism of the transcriptional regulation of the pho regulon.
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