- Development of a Novel D‑Lactic Acid Production Platform Based on Lactobacillus saerimneri TBRC 5746
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Kitisak Sansatchanon , Pipat Sudying , Peerada Promdonkoy , Yutthana Kingcha , Wonnop Visessanguan , Sutipa Tanapongpipat , Weerawat Runguphan , Kanokarn Kocharin
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J. Microbiol. 2023;61(9):853-863. Published online September 14, 2023
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DOI: https://doi.org/10.1007/s12275-023-00077-x
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 Abstract
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D-Lactic acid is a chiral, three-carbon organic acid, that bolsters the thermostability of polylactic acid. In this study, we
developed a microbial production platform for the high-titer production of D-lactic acid. We screened 600 isolates of lactic
acid bacteria (LAB) and identified twelve strains that exclusively produced D-lactic acid in high titers. Of these strains,
Lactobacillus saerimneri TBRC 5746 was selected for further development because of its homofermentative metabolism.
We investigated the effects of high temperature and the use of cheap, renewable carbon sources on lactic acid production and
observed a titer of 99.4 g/L and a yield of 0.90 g/g glucose (90% of the theoretical yield). However, we also observed L-lactic
acid production, which reduced the product’s optical purity. We then used CRISPR/dCas9-assisted transcriptional repression
to repress the two Lldh genes in the genome of L. saerimneri TBRC 5746, resulting in a 38% increase in D-lactic acid
production and an improvement in optical purity. This is the first demonstration of CRISPR/dCas9-assisted transcriptional
repression in this microbial host and represents progress toward efficient microbial production of D-lactic acid.
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Citations
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