- Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X
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Sun Hee Choi , Ki Hyun Ryu
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J. Microbiol. 2008;46(5):502-507. Published online October 31, 2008
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DOI: https://doi.org/10.1007/s12275-008-0078-2
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Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5’-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3’-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RTPCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta.
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Citations
Citations to this article as recorded by  - Validation of reference genes for quantifying changes in gene expression in virus-infected tobacco
Eseul Baek, Ju-Yeon Yoon, Peter Palukaitis
Virology.2017; 510: 29. CrossRef - Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study
Fater Youssef, Armelle Marais, Chantal Faure, Pascal Gentit, Thierry Candresse
Virology Journal.2011;[Epub] CrossRef
- Phylogenic Analysis of Alternaria brassicicola Producing Bioactive Metabolites
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Dong-Sun Jung , Yeo-Jung Na , Ki Hyun Ryu
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J. Microbiol. 2002;40(4):289-294.
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The fungal strain SW-3 having antimicrobial activity was isolated from soil of crucified plants in Pocheon, Kyungki-Do, Korea. Strain SW-3 was identified as Alternaria brassicicola by its morphological characteristics, and confirmed by the analysis of the 18S gene and ITS regions of rDNA. The fungus showed a similarity of 99% with Alternaria brassicicola in the 18S rDNA sequence analysis. A. brassicicola has been reported to produce an antitumor compound, called depudecin. We found that strain SW-3 produced antimicrobial metabolites, in addition to depudecin, during sporulation under different growth conditions. The metabolite of the isolated fungus was found to have strong antifungal activity against Microsporium canis and Trichophyton rubrum, and antibacterial activity against Staphylococcus aureus and Pseudomonas aerogenes. The amount and kind of metabolites produced by the isolate were affected by growth conditions such as nutrients and growth periods.
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