Journal of Microbiology

Keun-Shik Baik

2 Articles

Characterization of a Streptomycete Isolate Producing the Potent Cytotoxic Substance, Nonadecanoic Acid: Jin-cheol Yoo , Ji-Man Han , Seung-Kwan Nam , Keun-shik Baik , Jung-sun Jo , Chi-nam Seong; J. Microbiol. 2002;40(2):178-181.

Abstract PDF: Streptomycete isolate, strain M0137 showed cytotoxic effect on THP-1 cells. One of the purified substances produced from the strain was identified as nonadecanoic acid. Morphological and physiological properties, phylogenetic analysis, and genomic fingerprinting of strain M0137 were determined. Strain M0137 showed a high similarity with Streptomyces scabiei, phenotypically and phylogenetically. In contrast, genomic fingerprinting and G+C content analysis revealed that strain M0137 could be distinguished from S. scabiei ATCC49173 T . We propose to name strain M0137 as Streptomyces scabiei subsp. chosunensis.

An Improved Selective Isolation of Rare Actinomycetes from Forest Soil: Chi Nam Seong , Ji Heok Choi , Keun-Shik Baik; J. Microbiol. 2001;39(1):17-23.

Abstract PDF: Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70oC and phenol treatment of soil suspension were the most effective methods for the isolation of those microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

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