- Chitinibacter suncheonensis sp. nov., a Chitinolytic Bacterium from a Mud Flat in Suncheon Bay
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Sung Kyum Kim , Yong Ho Kim , Yu Seok Jeong , Han Beur Na , Jungho Kim , Keun Sik Baik , Han Dae Yun , Jung-Kul Lee , Hoon Kim
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J. Microbiol. 2012;50(6):1058-1062. Published online December 30, 2012
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DOI: https://doi.org/10.1007/s12275-012-2333-9
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A chitinolytic bacterium, designated strain SK16T, was isolated from a mud flat in Suncheon Bay, Republic of Korea. Strain SK16T is Gram-negative, strictly aerobic, motile by a polar flagellum, and short rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Chitinibacter and was most closely related to Chitinibacter tainanensis S1T (98.2% similarity). DNA-DNA hybridization analyses showed a low association value of 20.45±4.08% between them. The major cellular fatty acids, the G+C content of the genomic DNA, and the predominant quinone of the strain were summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c; 50.5%) and C12:0 (12.5%), 52.26 mol%, and Q-8, respectively. Based on the phylogenetic, chemotaxonomic, and phenotypic properties, strain SK16T represents a novel species of the genus Chitinibacter, for which the name Chitinibacter suncheonensis sp. nov. is proposed. The type strain is SK16T (=KCTC 23839T =DSM 25421T).
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- Characterization of a novel Bi-functional cold-adapted chitinase from Chitinibacter mangrovi FCG-7T for efficient production of N-acetyl-D-glucosamine
Chenxi Gu, Liyan Yang, Susu Han, Dengfeng Yang, Xiaodong Xu, WenChao Zhang, Mingguo Jiang, Lixia Pan Food Bioscience.2026; 75: 108168. CrossRef - Impact of moisture on microbial decomposition phenotypes and enzyme dynamics
Nicholas J Reichart, Sheryl Bell, Vanessa A Garayburu-Caruso, Natalie Sadler, Sharon Zhao, Kirsten S Hofmockel The ISME Journal.2025;[Epub] CrossRef - Validation List no. 226: valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef - Molecular Characterization of Four Alkaline Chitinases from Three Chitinolytic Bacteria Isolated from a Mudflat
Sung Kyum Kim, Jong Eun Park, Jong Min Oh, Hoon Kim International Journal of Molecular Sciences.2021; 22(23): 12822. CrossRef - The Draft Genome Sequence and Analysis of an Efficiently Chitinolytic Bacterium Chitinibacter sp. Strain GC72
Alei Zhang, Xiaofang Mo, Guoguang Wei, Ning Zhou, Sai Yang, Jie Chen, Yingying Wang, Kequan Chen, Pingkai Ouyang Current Microbiology.2020; 77(12): 3903. CrossRef - Characterization of extracellular chitinase from Chitinibacter sp. GC72 and its application in GlcNAc production from crayfish shell enzymatic degradation
Cong Gao, Alei Zhang, Kequan Chen, Zhikui Hao, Junmao Tong, Pingkai Ouyang Biochemical Engineering Journal.2015; 97: 59. CrossRef
- Screening and Characterization of a Cellulase Gene from the Gut Microflora of Abalone Using Metagenomic Library
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Duwoon Kim , Se-Na Kim , Keun Sik Baik , Seong Chan Park , Chae Hong Lim , Jong-Oh Kim , Tai-Sun Shin , Myung-Joo Oh , Chi Nam Seong
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J. Microbiol. 2011;49(1):141-145. Published online March 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0205-3
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A metagenomic fosmid library was constructed using genomic DNA isolated from abalone intestine. Screening of a library of 3,840 clones revealed a 36 kb insert of a cellulase positive clone (pAM11E10). A shotgun clone library was constructed using the positive clone (pAM11E10) and further screening of 3,840 shotgun clones with an approximately 5 kb insert size using a Congo red overlay revealed only one cellulase positive clone (pAM11L9). The pAM11L9 consisted of a 5,293-bp DNA sequence and three open reading frames (ORFs). Among the three ORFs, cellulase activity was only shown in the recombinant protein (CelAM11) coded by ORF3, which showed 100% identity with outer membrane protein A from Vibrio alginolyticus
12G01, but no significant sequence homology to known cellulases. The expressed protein (CelAM11) has a molecular weight of approximately 37 kDa and the highest CMC hydrolysis activity was observed at pH 7.0 and 37°C. The carboxymethyl cellulase activity was determined by zymogram active staining and different degraded product profiles for CelAM11 were obtained when cellotetraose and cellopentaose were used as the substrates, while no substrate hydrolysis was observed on oligosaccharides such as cellobiose and cellotriose.
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- Comparative genomics reveal distinct potential of Tamlana sp. S12 for algal polysaccharide degradation
Hai-Feng Xia, Xiao-Yu Jia, Yan-Xia Zhou, Zong-Jun Du, Da-Shuai Mu, Guan-Jun Chen Frontiers in Marine Science.2023;[Epub] CrossRef - A primer on metagenomics and next‐generation sequencing in fish gut microbiome research
Tina Kollannoor Johny, Rinu Madhu Puthusseri, Sarita Ganapathy Bhat Aquaculture Research.2021; 52(10): 4574. CrossRef - Characterization of Bacillus Species with Keratinase and Cellulase Properties Isolated from Feather Dumping Soil and Cockroach Gut
Sonika Sharma, Rajesh Kumar Prasad, Soumya Chatterjee, Angkita Sharma, Mohan G. Vairale, Kamlesh Kumar Yadav Proceedings of the National Academy of Sciences, India Section B: Biological Sciences.2019; 89(3): 1079. CrossRef - Prospects of Metagenomic Cellulases for Converting Lignocellulosic Biomass into Bio-ethanol
Sangeeta Pandey Journal of Pure and Applied Microbiology.2017; 11(2): 1079. CrossRef - Metagenomics for the development of new biocatalysts to advance lignocellulose saccharification for bioeconomic development
Salvatore Montella, Antonella Amore, Vincenza Faraco Critical Reviews in Biotechnology.2016; 36(6): 998. CrossRef - Identification and Characterization of Carboxyl Esterases of Gill Chamber-Associated Microbiota in the Deep-Sea Shrimp Rimicaris exoculata by Using Functional Metagenomics
María Alcaide, Anatoli Tchigvintsev, Mónica Martínez-Martínez, Ana Popovic, Oleg N. Reva, Álvaro Lafraya, Rafael Bargiela, Taras Y. Nechitaylo, Ruth Matesanz, Marie-Anne Cambon-Bonavita, Mohamed Jebbar, Michail M. Yakimov, Alexei Savchenko, Olga V. Golysh Applied and Environmental Microbiology.2015; 81(6): 2125. CrossRef - Purification and characterization of a carboxymethyl cellulase from Artemia salina
Hyun Woo Zin, Kwang-Hyun Park, Tae Jin Choi Biochemical and Biophysical Research Communications.2014; 443(1): 194. CrossRef - Cloning and functional characterization of endo-β-1,4-glucanase gene from metagenomic library of vermicompost
Muhammad Yasir, Haji Khan, Syed Sikander Azam, Amar Telke, Seon Won Kim, Young Ryun Chung Journal of Microbiology.2013; 51(3): 329. CrossRef - Processive and nonprocessive cellulases for biofuel production—lessons from bacterial genomes and structural analysis
David B. Wilson Applied Microbiology and Biotechnology.2012; 93(2): 497. CrossRef - Gene Cloning of Endoglucanase Cel5A from Cellulose-Degrading Paenibacillus xylanilyticus KJ-03 and Purification and Characterization of the Recombinant Enzyme
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- Diversity of Bacterial Community in Freshwater of Woopo Wetland
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Keun Sik Baik , Seong Chan Park , Eun Mi Kim , Kyung Sook Bae , Jae-Hyung Ahn , Jong-Ok Ka , Jongsik Chun , Chi Nam Seong
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J. Microbiol. 2008;46(6):647-655. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0135-x
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672
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Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria.
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Paenibacillus wooponensis sp. nov., isolated from wetland freshwater
Keun Sik Baik, Han Na Choe, Seong Chan Park, Eun Mi Kim, Chi Nam Seong
International Journal of Systematic and Evolutionary Microbiology
.2011; 61(11): 2763. CrossRef -
Paenibacillus rigui sp. nov., isolated from a freshwater wetland
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- Acinetobacter soli sp. nov., Isolated from Forest Soil
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Duwoon Kim , Keun Sik Baik , Mi Sun Kim , Seong Chan Park , Seon Suk Kim , Moon Soo Rhee , Young Se Kwak , Chi Nam Seong
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J. Microbiol. 2008;46(4):396-401. Published online August 31, 2008
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DOI: https://doi.org/10.1007/s12275-008-0118-y
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A non-motile and rod shaped bacterium, designated strain B1T, was isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C18:1 ω9c; 42%) and hexadecanoic acid (C16:0; 25.9%) and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1T formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961T (98.6% sequence similarity), followed by A. baumannii DSM 30007T (97.4%), A. calcoaceticus DSM 30006T (97.0%), and 3 genomic species (96.8~7.6%). Phenotypic characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1T from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is B1T (= KCTC 22184T= JCM 15062T).
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- InhA-Like Protease Secreted by Bacillus sp. S17110 Inhabited in Turban Shell
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Sang Chul Jung , Hyoung-Rok Paik , Mi Sun Kim , Keun Sik Baik , Woo-Yiel Lee , Chi Nam Seong , Sang Ki Choi
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J. Microbiol. 2007;45(5):402-408.
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DOI: https://doi.org/2597 [pii]
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Abstract
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A strain producing a potent protease was isolated from turban shell. The strain was identified as Bacillus sp. S17110 based on phylogenetic analysis. The enzyme was purified from culture supernatant of Bacillus sp. S17110 to homogeneity by ammonium sulfate precipitation, SP-Sepharose, and DEAE-Sepharose anion exchange chromatography. Protease activity of the purified protein against casein was found to be stable at pH 7 to pH 10 and around 50°C. Approximately 70% of proteolytic activity of the enzyme was detected either in the presence of 100 mM SDS or Tween 20. The enzyme activity was enhanced in the presence of Ca2+, Zn2+, Mg2+, but was inhibited by EDTA, indicating that it requires metal for its activity. The purified
enzyme was found to be a monomeric protein with a molecular mass of 75 kDa, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography. The purified enzyme was analyzed through peptide fingerprint mass spectra generated from matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and a BLAST search, and identified as immune inhibitor A (inhA) deduced from nucleotide sequence of B. cereus G9241. Since InhA was identified as protease that cleave antibacterial proteins found in insect, inhA-like protease purified from Bacillus sp. S17110 might be pathogenic to sea invertebrates.
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