- Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
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Dayeong Bae , Hana Hyeon , Eunkyoung Shin , Ji&# , Kangseok Lee
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J. Microbiol. 2023;61(2):211-220. Published online February 22, 2023
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DOI: https://doi.org/10.1007/s12275-023-00013-z
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85
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 Abstract
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RNase E is an essential enzyme in Escherichia coli. The cleavage site of this single-stranded specific endoribonuclease is
well-characterized in many RNA substrates. Here, we report that the upregulation of RNase E cleavage activity by a mutation
that affects either RNA binding (Q36R) or enzyme multimerization (E429G) was accompanied by relaxed cleavage specificity.
Both mutations led to enhanced RNase E cleavage in RNA I, an antisense RNA of ColE1-type plasmid replication,
at a major site and other cryptic sites. Expression of a truncated RNA I with a major RNase E cleavage site deletion at the
5′-end (RNA I-
5) resulted in an approximately twofold increase in the steady-state levels of RNA I-
5 and the copy number
of ColE1-type plasmid in E. coli cells expressing wild-type or variant RNase E compared to those expressing RNA I. These
results
indicate that RNA I-
5 does not efficiently function as an antisense RNA despite having a triphosphate group at the
5′-end, which protects the RNA from ribonuclease attack. Our study suggests that increased cleavage rates of RNase E lead
to relaxed cleavage specificity on RNA I and the inability of the cleavage product of RNA I as an antisense regulator in vivo
does not stem from its instability by having 5′-monophosphorylated end.
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Citations
Citations to this article as recorded by  - Engineering an Escherichia coli based in vivo mRNA manufacturing platform
Edward Curry, George Muir, Jixin Qu, Zoltán Kis, Martyn Hulley, Adam Brown
Biotechnology and Bioengineering.2024; 121(6): 1912. CrossRef
- Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6
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Younkyung Choi , Minju Joo , Wooseok Song , Minho Lee , Hana Hyeon , Hyun-Lee Kim , Ji-Hyun Yeom , Kangseok Lee , Eunkyoung Shin
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J. Microbiol. 2022;60(12):1162-1167. Published online November 24, 2022
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DOI: https://doi.org/10.1007/s12275-022-2437-9
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83
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2
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Abstract
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Ribosomes composed of genome-encoded heterogeneous
rRNAs are implicated in the rapid adaptation of bacterial
cells to environmental changes. A previous study showed that
ribosomes bearing the most heterogeneous rRNAs expressed
from the rrnI operon (I-ribosomes) are implicated in the preferential
translation of a subset of mRNAs, including hspA
and tpiA, in Vibrio vulnificus CMCP6. In this study, we show
that HspA nascent peptides were predominantly bound to
I-ribosomes. Specifically, I-ribosomes were enriched more
than two-fold in ribosomes that were pulled down by immunoprecipitation
of HspA peptides compared with the proportion
of I-ribosomes in crude ribosomes and ribosomes pulled
down by immunoprecipitation of RNA polymerase subunit
ß peptides in the wild-type (WT) and rrnI-completed strains.
Other methods that utilized the incorporation of an affinity
tag in 23S rRNA or chimeric rRNA tethering 16S and 23S
rRNAs, which generated specialized functional ribosomes
in Escherichia coli, did not result in functional I-ribosomes
in V. vulnificus CMCP6. This study provides direct evidence
of the preferential translation of hspA mRNA by I-ribosomes.
-
Citations
Citations to this article as recorded by - Functional conservation of specialized ribosomes bearing genome-encoded variant rRNAs in Vibrio species
Younkyung Choi, Eunkyoung Shin, Minho Lee, Ji-Hyun Yeom, Kangseok Lee, Bashir Sajo Mienda
PLOS ONE.2023; 18(12): e0289072. CrossRef - Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef
- [Protocol] Development of DNA aptamers specific for small therapeutic peptides using a modified SELEX method
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Jaemin Lee , Minkyung Ryu , Dayeong Bae , Hong-Man Kim , Seong-il Eyun , Jeehyeon Bae , Kangseok Lee
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J. Microbiol. 2022;60(7):659-667. Published online June 22, 2022
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DOI: https://doi.org/10.1007/s12275-022-2235-4
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81
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6
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6
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Abstract
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Aptamers are short single-stranded DNA or RNA oligonucleotides
capable of binding with high affinity and specificity
to target molecules. Because of their durability and ease of synthesis,
aptamers are used in a wide range of biomedical fields,
including the diagnosis of diseases and targeted delivery of
therapeutic agents. The aptamers were selected using a process
called systematic evolution of ligands by exponential enrichment
(SELEX), which has been improved for various research
purposes since its development in 1990. In this protocol,
we describe a modified SELEX method that rapidly produces
high aptamer screening yields using two types of magnetic
beads. Using this method, we isolated an aptamer that
specifically binds to an antimicrobial peptide. We suggest that
by conjugating a small therapeutic-specific aptamer to a gold
nanoparticle-based delivery system, which enhances the stability
and intracellular delivery of peptides, aptamers selected
by our method can be used for the development of therapeutic
agents utilizing small therapeutic peptides.
-
Citations
Citations to this article as recorded by - Recent approaches in the application of antimicrobial peptides in food preservation
Satparkash Singh, Bhavna Jha, Pratiksha Tiwari, Vinay G. Joshi, Adarsh Mishra, Yashpal Singh Malik
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef - Design and application of microfluidics in aptamer SELEX and Aptasensors
Shikun Zhang, Yingming Zhang, Zhiyuan Ning, Mengxia Duan, Xianfeng Lin, Nuo Duan, Zhouping Wang, Shijia Wu
Biotechnology Advances.2024; 77: 108461. CrossRef - Nanogenosensors based on aptamers and peptides for bioelectrochemical cancer detection: an overview of recent advances in emerging materials and technologies
Babak Mikaeeli Kangarshahi, Seyed Morteza Naghib
Discover Applied Sciences.2024;[Epub] CrossRef - Recent progress of SELEX methods for screening nucleic acid aptamers
Chao Zhu, Ziru Feng, Hongwei Qin, Lu Chen, Mengmeng Yan, Linsen Li, Feng Qu
Talanta.2024; 266: 124998. CrossRef - Aptamer-conjugated gold nanoparticles platform as the intracellular delivery of antibodies for cancer therapy
Ji-Hyun Yeom, Eunkyoung Shin, Hanyong Jin, Haifeng Liu, Yongyang Luo, Youngwoo Nam, Minkyung Ryu, Wooseok Song, Heeyoun Chi, Jeongkyu Kim, Kangseok Lee, Jeehyeon Bae
Journal of Industrial and Engineering Chemistry.2023; 126: 480. CrossRef - Regulation of transforming growth factor-β signaling as a therapeutic approach to treating colorectal cancer
Jana Maslankova, Ivana Vecurkovska, Miroslava Rabajdova, Jana Katuchova, Milos Kicka, Michala Gayova, Vladimir Katuch
World Journal of Gastroenterology.2022; 28(33): 4744. CrossRef
- Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
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Jaeyeong Park , Eunkyoung Shin , Ji-Hyun Yeom , Younkyung Choi , Minju Joo , Minho Lee , Je Hyeong Kim , Jeehyeon Bae , Kangseok Lee
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J. Microbiol. 2022;60(1):128-136. Published online December 29, 2021
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DOI: https://doi.org/10.1007/s12275-022-1620-3
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170
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15
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15
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Abstract
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Acinetobacter baumannii causes multidrug resistance, leading
to fatal infections in humans. In this study, we showed that
Lys AB2 P3-His–a hexahistidine-tagged form of an antimicrobial
peptide (AMP) loaded onto DNA aptamer-functionalized
gold nanoparticles (AuNP-Apt)–can effectively inhibit
A. baumannii infection in mice. When A. baumannii-infected
mice were intraperitoneally injected with AuNP-Apt loaded
with Lys AB2 P3-His, a marked reduction in A. baumannii
colonization was observed in the mouse organs, leading to
prominently increased survival time and rate of the mice compared
to those of the control mice treated with AuNP-Apt or
Lys AB2 P3-His only. This study shows that AMPs loaded
onto AuNP-Apt could be an effective therapeutic tool against
infections caused by multidrug-resistant pathogenic bacteria
in humans.
-
Citations
Citations to this article as recorded by - Challenges and Emerging Molecular Approaches in Combating Antimicrobial Resistance
Gene Philip Levee Ynion, Christian Jay Rosal, Arvin Zulueta, Angelo Ordanel, Christopher Marlowe Caipang
Journal of Bacteriology and Virology.2024; 54(1): 12. CrossRef - Gold Nanoparticles and Antimicrobial Peptides: A Novel Combination
Şule Balcı, Bengü Ergüden
ChemistrySelect.2024;[Epub] CrossRef - Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification
María Guadalupe Córdova-Espinoza, Rosa González-Vázquez, Rolando Rafik Barron-Fattel, Raquel Gónzalez-Vázquez, Marco Antonio Vargas-Hernández, Exsal Manuel Albores-Méndez, Ana Laura Esquivel-Campos, Felipe Mendoza-Pérez, Lino Mayorga-Reyes, María Angélica
International Journal of Molecular Sciences.2024; 25(2): 1257. CrossRef - Antimicrobial Peptide Delivery Systems as Promising Tools Against Resistant Bacterial Infections
Kamila Botelho Sampaio de Oliveira, Michel Lopes Leite, Nadielle Tamires Moreira Melo, Letícia Ferreira Lima, Talita Cristina Queiroz Barbosa, Nathalia Lira Carmo, Douglas Afonso Bittencourt Melo, Hugo Costa Paes, Octávio Luiz Franco
Antibiotics.2024; 13(11): 1042. CrossRef - Optimizing Treatment for Carbapenem-Resistant Acinetobacter baumannii Complex Infections: A Review of Current Evidence
Seong Jin Choi, Eu Suk Kim
Infection & Chemotherapy.2024; 56(2): 171. CrossRef - Advances in skin gene therapy: utilizing innovative dressing scaffolds for wound healing, a comprehensive review
Fatemeh Karimzadeh, Elahe Soltani Fard, Akram Nadi, Rahim Malekzadeh, Fatemeh Elahian, Seyed Abbas Mirzaei
Journal of Materials Chemistry B.2024; 12(25): 6033. CrossRef - Colistin Resistance Mechanism and Management Strategies of Colistin-Resistant Acinetobacter baumannii Infections
Md Minarul Islam, Da Eun Jung, Woo Shik Shin, Man Hwan Oh
Pathogens.2024; 13(12): 1049. CrossRef - Progress in Programmable DNA-Aided Self-Assembly of the Master Frame of a Drug Delivery System
Gary Q. Yang, Weibin Cai, Zhiwen Zhang, Yujun Wang
ACS Applied Bio Materials.2023; 6(12): 5125. CrossRef - Neglected Zoonotic Diseases: Advances in the Development of Cell-Penetrating and Antimicrobial Peptides against Leishmaniosis and Chagas Disease
Sara M. Robledo, Silvia Pérez-Silanes, Celia Fernández-Rubio, Ana Poveda, Lianet Monzote, Víctor M. González, Paloma Alonso-Collado, Javier Carrión
Pathogens.2023; 12(7): 939. CrossRef - Applications and Challenges of Bacteriostatic Aptamers in the Treatment of Common Pathogenic Bacteria Infections
Diandian Li, Yuan Su, Jie Li, Rong Liu, Bing Fang, Jingjing He, Wentao Xu, Longjiao Zhu
Biomacromolecules.2023; 24(11): 4568. CrossRef - Promising Acinetobacter baumannii Vaccine Candidates and Drug Targets in Recent Years
Yong Chiang Tan, Chandrajit Lahiri
Frontiers in Immunology.2022;[Epub] CrossRef - Advances and Perspective on Antimicrobial Nanomaterials for Biomedical Applications
Preeti Garg, Prerna Attri, Rohit Sharma, Moondeep Chauhan, Ganga Ram Chaudhary
Frontiers in Nanotechnology.2022;[Epub] CrossRef - Aptamer Decorated Emodin Nanoparticles-Assisted Delivery of Dermcidin-Derived Peptide DCD-1L: Photoactive Bio-Theragnostic Agent for Enterococcus Faecalis Biofilm Destruction
Maryam Pourhajibagher, Abbas Bahador
SSRN Electronic Journal .2022;[Epub] CrossRef - Development of DNA aptamers specific for small therapeutic peptides using a modified SELEX method
Jaemin Lee, Minkyung Ryu, Dayeong Bae, Hong-Man Kim, Seong-il Eyun, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(7): 659. CrossRef - Aptamer decorated emodin nanoparticles-assisted delivery of dermcidin-derived peptide DCD-1L: Photoactive bio-theragnostic agent for Enterococcus faecalis biofilm destruction
Maryam Pourhajibagher, Abbas Bahador
Photodiagnosis and Photodynamic Therapy.2022; 39: 103020. CrossRef
- Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
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Jaejin Lee , Eunkyoung Shin , Jaeyeong Park , Minho Lee , Kangseok Lee
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J. Microbiol. 2021;59(12):1133-1141. Published online November 9, 2021
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DOI: https://doi.org/10.1007/s12275-021-1518-5
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85
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4
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5
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Abstract
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RraA, a protein regulator of RNase E activity, plays a unique
role in modulating the mRNA abundance in Escherichia coli.
The marine pathogenic bacterium Vibrio vulnificus also possesses
homologs of RNase E (VvRNase E) and RraA (VvRraA1
and VvRraA2). However, their physiological roles have not
yet been investigated. In this study, we demonstrated that
VvRraA1 expression levels affect the pathogenicity of V. vulnificus.
Compared to the wild-type strain, the VvrraA1-deleted
strain (ΔVvrraA1) showed decreased motility, invasiveness,
biofilm formation ability as well as virulence in mice; these
phenotypic changes of ΔVvrraA1 were restored by the exogenous
expression of VvrraA1. Transcriptomic analysis indicated
that VvRraA1 expression levels affect the abundance
of a large number of mRNA species. Among them, the halflives
of mRNA species encoding virulence factors (e.g., smcR
and htpG) that have been previously shown to affect VvrraA1
expression-dependent phenotypes were positively correlated
with VvrraA1 expression levels. These findings suggest that
VvRraA1 modulates the pathogenicity of V. vulnificus by regulating
the abundance of a subset of mRNA species.
-
Citations
Citations to this article as recorded by -
Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in
Vibrio alginolyticus
Huizhen Chen, Qian Gao, Bing Liu, Ying Zhang, Jianxiang Fang, Songbiao Wang, Youqi Chen, Chang Chen, Nicolas E. Buchler
mSphere.2024;[Epub] CrossRef - Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Seungmok Han, Ji-Won Byun, Minho Lee
Journal of Microbiology.2024; 62(1): 33. CrossRef - Eco-Evolutionary Drivers of Vibrio parahaemolyticus Sequence Type 3 Expansion: Retrospective Machine Learning Approach
Amy Marie Campbell, Chris Hauton, Ronny van Aerle, Jaime Martinez-Urtaza
JMIR Bioinformatics and Biotechnology.2024; 5: e62747. CrossRef - Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
- Trans-acting regulators of ribonuclease activity
-
Jaejin Lee , Minho Lee , Kangseok Lee
-
J. Microbiol. 2021;59(4):341-359. Published online March 29, 2021
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DOI: https://doi.org/10.1007/s12275-021-0650-6
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76
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3
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4
Crossref
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Abstract
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RNA metabolism needs to be tightly regulated in response to
changes in cellular physiology. Ribonucleases (RNases) play
an essential role in almost all aspects of RNA metabolism, including
processing, degradation, and recycling of RNA molecules.
Thus, living systems have evolved to regulate RNase
activity at multiple levels, including transcription, post-transcription,
post-translation, and cellular localization. In addition,
various trans-acting regulators of RNase activity have
been discovered in recent years. This review focuses on the
physiological roles and underlying mechanisms of trans-acting
regulators of RNase activity.
-
Citations
Citations to this article as recorded by - Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Seungmok Han, Ji-Won Byun, Minho Lee
Journal of Microbiology.2024; 62(1): 33. CrossRef - Insights into the metabolism, signaling, and physiological effects of 2’,3’-cyclic nucleotide monophosphates in bacteria
Nick J. Marotta, Emily E. Weinert
Critical Reviews in Biochemistry and Molecular Biology.2023; 58(2-6): 118. CrossRef - Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef
- Rediscovery of antimicrobial peptides as therapeutic agents
-
Minkyung Ryu , Jaeyeong Park , Ji-Hyun Yeom , Minju Joo , Kangseok Lee
-
J. Microbiol. 2021;59(2):113-123. Published online February 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-0649-z
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86
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28
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28
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Abstract
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In recent years, the occurrence of antibiotic-resistant pathogens
is increasing rapidly. There is growing concern as
the development of antibiotics is slower than the increase in
the resistance of pathogenic bacteria. Antimicrobial peptides
(AMPs) are promising alternatives to antibiotics. Despite their
name, which implies their antimicrobial activity, AMPs have
recently been rediscovered as compounds having antifungal,
antiviral, anticancer, antioxidant, and insecticidal effects.
Moreover, many AMPs are relatively safe from toxic side effects
and the generation of resistant microorganisms due to
their target specificity and complexity of the mechanisms underlying
their action. In this review, we summarize the history,
classification, and mechanisms of action of AMPs, and
provide descriptions of AMPs undergoing clinical trials. We
also discuss the obstacles associated with the development of
AMPs as therapeutic agents and recent strategies formulated
to circumvent these obstacles.
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Citations
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Muhammad Asif, Shuang Liang, Hu RenJian, Xin Xie, Zhibo Zhao
Physiological and Molecular Plant Pathology.2025; 136: 102506. CrossRef - Mechanistic insights on the antibacterial action of the kyotorphin peptide derivatives revealed by in vitro studies and Galleria mellonella proteomic analysis
Vitor M. de Andrade, Vitor D.M. de Oliveira, Uilla Barcick, Vasanthakumar G. Ramu, Montserrat Heras, Eduard R. Bardají, Miguel A.R.B. Castanho, André Zelanis, Aline Capella, Juliana C. Junqueira, Katia Conceição
Microbial Pathogenesis.2024; 189: 106607. CrossRef - Antimicrobial Peptides Derived from Bacteria: Classification, Sources, and Mechanism of Action against Multidrug-Resistant Bacteria
Raynichka Mihaylova-Garnizova, Slavena Davidova, Yordan Hodzhev, Galina Satchanska
International Journal of Molecular Sciences.2024; 25(19): 10788. CrossRef - A Zeolitic Imidazolate Framework-Based Antimicrobial Peptide Delivery System with Enhanced Anticancer Activity and Low Systemic Toxicity
Jingwen Jiang, Kaderya Kaysar, Yanzhu Pan, Lijie Xia, Jinyao Li
Pharmaceutics.2024; 16(12): 1591. CrossRef - Enhancing Antimicrobial Peptide Activity through Modifications of Charge, Hydrophobicity, and Structure
Przemysław Gagat, Michał Ostrówka, Anna Duda-Madej, Paweł Mackiewicz
International Journal of Molecular Sciences.2024; 25(19): 10821. CrossRef - Studies of antibacterial activity (in vitro and in vivo) and mode of action for des-acyl tridecaptins (DATs)
Cédric Couturier, Quentin Ronzon, Giulia Lattanzi, Iain Lingard, Sebastien Coyne, Veronique Cazals, Nelly Dubarry, Stephane Yvon, Corinne Leroi-Geissler, Obdulia Rabal Gracia, Joanne Teague, Sylvie Sordello, David Corbett, Caroline Bauch, Chantal Monlong,
European Journal of Medicinal Chemistry.2024; 265: 116097. CrossRef - Antimicrobial Peptides: The Game-Changer in the Epic Battle Against Multidrug-Resistant Bacteria
Helal F. Hetta, Nizar Sirag, Shumukh M. Alsharif, Ahmad A. Alharbi, Tala T. Alkindy, Alanoud Alkhamali, Abdullah S. Albalawi, Yasmin N. Ramadan, Zainab I. Rashed, Fawaz E. Alanazi
Pharmaceuticals.2024; 17(11): 1555. CrossRef - Proteomic and antimicrobial peptide analyses of Buffalo colostrum and mature Milk whey: A comparative study
Runfeng Liu, Yuan Yang, Yue Zhang, Qinqiang Sun, Pingchuan Zhu, Huiyan Xu, Wei Zheng, Yangqing Lu, Qiang Fu
Food Chemistry.2024; 448: 139119. CrossRef - Contemporary Insights into Non-typhoidal Salmonella: Understanding the Pathogenicity, Infection Mechanisms, and Strategies for Prevention and Control
Minho Lee
Journal of Bacteriology and Virology.2024; 54(4): 247. CrossRef - Peptide Flexibility and the Hydrophobic Moment are Determinants to Evaluate the Clinical Potential of Magainins
Daniel Balleza
The Journal of Membrane Biology.2023; 256(4-6): 317. CrossRef - Evaluation of glycyl-arginine and lysyl-aspartic acid dipeptides for their antimicrobial, antibiofilm, and anticancer potentials
Handan Sevim Akan, Gülcan Şahal, Tuğçe Deniz Karaca, Özer Aylin Gürpınar, Meltem Maraş, Alev Doğan
Archives of Microbiology.2023;[Epub] CrossRef - The biological role of charge distribution in linear antimicrobial peptides
Harry Morales Duque, Gisele Rodrigues, Lucas Souza Santos, Octávio Luiz Franco
Expert Opinion on Drug Discovery.2023; 18(3): 287. CrossRef - Discovery of Lactomodulin, a Unique Microbiome-Derived Peptide That Exhibits Dual Anti-Inflammatory and Antimicrobial Activity against Multidrug-Resistant Pathogens
Walaa K. Mousa, Rose Ghemrawi, Tareq Abu-Izneid, Azza Ramadan, Farah Al-Marzooq
International Journal of Molecular Sciences.2023; 24(8): 6901. CrossRef - An injectable thermosensitive hydrogel with a self-assembled peptide coupled with an antimicrobial peptide for enhanced wound healing
Tianqi Feng, Hongyan Wu, Wendi Ma, Zhaoguo Wang, Chunli Wang, Yilong Wang, Siyao Wang, Mei Zhang, Linlin Hao
Journal of Materials Chemistry B.2022; 10(32): 6143. CrossRef - Dissecting the relationship between antimicrobial peptides and mesenchymal stem cells
Amandda Évelin Silva-Carvalho, Marlon Henrique Cardoso, Thuany Alencar-Silva, Gabriela Muller Reche Bogéa, Juliana Lott Carvalho, Octávio Luiz Franco, Felipe Saldanha-Araujo
Pharmacology & Therapeutics.2022; 233: 108021. CrossRef - A Solid Support‐Based Synthetic Strategy for the Site‐Selective Functionalization of Peptides with Organometallic Half‐Sandwich Moieties
Dianna Truong, Nelson Y. S. Lam, Meder Kamalov, Mie Riisom, Stephen M. F. Jamieson, Paul W. R. Harris, Margaret A. Brimble, Nils Metzler‐Nolte, Christian G. Hartinger
Chemistry – A European Journal.2022;[Epub] CrossRef - Bacteria-derived chimeric toxins as potential anticancer agents
Saeed Khoshnood, Hadis Fathizadeh, Foroogh Neamati, Babak Negahdari, Piyush Baindara, Mohd Azmuddin Abdullah, Mohammad Hossein Haddadi
Frontiers in Oncology.2022;[Epub] CrossRef - Identification of antimicrobial peptides from the human gut microbiome using deep learning
Yue Ma, Zhengyan Guo, Binbin Xia, Yuwei Zhang, Xiaolin Liu, Ying Yu, Na Tang, Xiaomei Tong, Min Wang, Xin Ye, Jie Feng, Yihua Chen, Jun Wang
Nature Biotechnology.2022; 40(6): 921. CrossRef - Anticancer Peptide Prediction via Multi-Kernel CNN and Attention Model
Xiujin Wu, Wenhua Zeng, Fan Lin, Peng Xu, Xinzhu Li
Frontiers in Genetics.2022;[Epub] CrossRef - Multitalented Synthetic Antimicrobial Peptides and Their Antibacterial, Antifungal and Antiviral Mechanisms
Tania Vanzolini, Michela Bruschi, Andrea C. Rinaldi, Mauro Magnani, Alessandra Fraternale
International Journal of Molecular Sciences.2022; 23(1): 545. CrossRef - Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(1): 128. CrossRef - Polymeric Coatings and Antimicrobial Peptides as Efficient Systems for Treating Implantable Medical Devices Associated-Infections
Irina Negut, Bogdan Bita, Andreea Groza
Polymers.2022; 14(8): 1611. CrossRef - Development of DNA aptamers specific for small therapeutic peptides using a modified SELEX method
Jaemin Lee, Minkyung Ryu, Dayeong Bae, Hong-Man Kim, Seong-il Eyun, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(7): 659. CrossRef - In Vivo Behavior of the Antibacterial Peptide Cyclo[RRRWFW], Explored Using a 3-Hydroxychromone-Derived Fluorescent Amino Acid
Sergii Afonin, Serhii Koniev, Laetitia Préau, Masanari Takamiya, Alexander V. Strizhak, Oleg Babii, Andrii Hrebonkin, Vasyl G. Pivovarenko, Margitta Dathe, Ferdinand le Noble, Sepand Rastegar, Uwe Strähle, Anne S. Ulrich, Igor V. Komarov
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Kai Ren, Xiumei Chi, Tiange Wu, Mujie Kan, Jiankai Liu, Jiayue Cui
International Journal of Peptide Research and Therapeutics.2021; 27(4): 2199. CrossRef - Lysozyme-like Protein Produced by Bifidobacterium longum Regulates Human Gut Microbiota Using In Vitro Models
Mingzhu Du, Xinqiang Xie, Shuanghong Yang, Ying Li, Tong Jiang, Juan Yang, Longyan Li, Yunxiao Huang, Qingping Wu, Wei Chen, Jumei Zhang
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Pasquale Marrazzo, Valeria Pizzuti, Silvia Zia, Azzurra Sargenti, Daniele Gazzola, Barbara Roda, Laura Bonsi, Francesco Alviano
Antibiotics.2021; 10(7): 750. CrossRef - Therapeutic Effect of an Antibody-Derived Peptide in a Galleria mellonella Model of Systemic Candidiasis
Emerenziana Ottaviano, Elisa Borghi, Laura Giovati, Monica Falleni, Delfina Tosi, Walter Magliani, Giulia Morace, Stefania Conti, Tecla Ciociola
International Journal of Molecular Sciences.2021; 22(20): 10904. CrossRef
- RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
-
Jaejin Lee , Dong-Ho Lee , Che Ok Jeon , Kangseok Lee
-
J. Microbiol. 2019;57(10):910-917. Published online September 30, 2019
-
DOI: https://doi.org/10.1007/s12275-019-9354-6
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72
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10
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9
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Abstract
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Studies have shown that many enzymes involved in glycolysis
are upregulated in Escherichia coli endoribonuclease G (rng)
null mutants. However, the molecular mechanisms underlying
the RNase G-associated regulation of glycolysis have
not been characterized. Here, we show that RNase G cleaves
the 5untranslated region of triosephosphate isomerase A
(tpiA) mRNA, leading to destabilization of the mRNA in E.
coli. Nucleotide substitutions within the RNase G cleavage
site in the genome resulted in altered tpiA mRNA stability,
indicating that RNase G activity influences tpiA mRNA
abundance. In addition, we observed that tpiA expression was
enhanced, whereas that of RNase G was decreased, in E. coli
cells grown anaerobically. Our findings suggest that RNase
G negatively regulates tpiA mRNA abundance in response
to oxygen availability in E. coli.
-
Citations
Citations to this article as recorded by - Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef - Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6
Younkyung Choi, Minju Joo, Wooseok Song, Minho Lee, Hana Hyeon, Hyun-Lee Kim, Ji-Hyun Yeom, Kangseok Lee, Eunkyoung Shin
Journal of Microbiology.2022; 60(12): 1162. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression
Minho Lee, Minkyung Ryu, Minju Joo, Young-Jin Seo, Jaejin Lee, Hong-Man Kim, Eunkyoung Shin, Ji-Hyun Yeom, Yong-Hak Kim, Jeehyeon Bae, Kangseok Lee, William Navarre
PLOS Pathogens.2021; 17(2): e1009263. CrossRef - Trans-acting regulators of ribonuclease activity
Jaejin Lee, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(4): 341. CrossRef - The effect of two ribonucleases on the production of Shiga toxin and stx-bearing bacteriophages in Enterohaemorrhagic Escherichia coli
Patricia B. Lodato
Scientific Reports.2021;[Epub] CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef - An alternative miRISC targets a cancer‐associated coding sequence mutation in FOXL2
Eunkyoung Shin, Hanyong Jin, Dae‐Shik Suh, Yongyang Luo, Hye‐Jeong Ha, Tae Heon Kim, Yoonsoo Hahn, Seogang Hyun, Kangseok Lee, Jeehyeon Bae
The EMBO Journal.2020;[Epub] CrossRef - The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
Scientific Reports.2019;[Epub] CrossRef
- Genome analysis of Rubritalea profundi SAORIC-165T, the first deep-sea verrucomicrobial isolate, from the northwestern Pacific Ocean
-
Jaeho Song , Ilnam Kang , Yochan Joung , Susumu Yoshizawa , Ryo Kaneko , Kenshiro Oshima , Masahira Hattori , Koji Hamasaki , Soochan Kim , Kangseok Lee , Jang-Cheon Cho
-
J. Microbiol. 2019;57(5):413-422. Published online February 26, 2019
-
DOI: https://doi.org/10.1007/s12275-019-8712-8
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76
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2
Web of Science
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2
Crossref
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Abstract
-
Although culture-independent studies have shown the presence
of Verrucomicrobia in the deep sea, verrucomicrobial
strains from deep-sea environments have been rarely cultured
and characterized. Recently, Rubritalea profundi SAORIC-
165T, a psychrophilic bacterium of the phylum Verrucomicrobia,
was isolated from a depth of 2,000 m in the northwestern
Pacific Ocean. In this study, the genome sequence
of R. profundi SAORIC-165T, the first deep-sea verrucomicrobial
isolate, is reported with description of the genome
properties and comparison to surface-borne Rubritalea genomes.
The draft genome consisted of four contigs with an
entire size of 4,167,407 bp and G+C content of 47.5%. The
SAORIC-165T genome was predicted to have 3,844 proteincoding
genes and 45 non-coding RNA genes. The genome
contained a repertoire of metabolic pathways, including the
Embden-Meyerhof-Parnas pathway, pentose phosphate pathway,
tricarboxylic acid cycle, assimilatory sulfate reduction,
and biosynthesis of nicotinate/nicotinamide, pantothenate/
coenzyme A, folate, and lycopene. The comparative genomic
analyses with two surface-derived Rubritalea genomes showed
that the SAORIC-165T genome was enriched in genes involved
in transposition of mobile elements, signal transduction, and
carbohydrate metabolism, some of which might be related to
bacterial enhancement of ecological fitness in the deep-sea
environment. Amplicon sequencing of 16S rRNA genes from
the water column revealed that R. profundi-related phylotypes
were relatively abundant at 2,000 m and preferred a
particle-associated life style in the deep sea. These findings
suggest that R. profundi represents a genetically unique and
ecologically relevant verrucomicrobial group well adapted
to the deep-sea environment.
-
Citations
Citations to this article as recorded by - Cultivation of deep-sea bacteria from the Northwest Pacific Ocean and characterization of Limnobacter profundi sp. nov., a phenol-degrading bacterium
Mirae Kim, Jaeho Song, Seung Yeol Shin, Kazuhiro Kogure, Ilnam Kang, Jang-Cheon Cho
Frontiers in Marine Science.2024;[Epub] CrossRef - Profiling Branchial Bacteria of Atlantic Salmon (Salmo salar L.) Following Exposure to Antimicrobial Agents
Joel Slinger, James W. Wynne, Mark B. Adams
Frontiers in Animal Science.2021;[Epub] CrossRef
- REVIEW] Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
-
Inseong Jo , Jin-Sik Kim , Yongbin Xu , Jaekyung Hyun , Kangseok Lee , Nam-Chul Ha
-
J. Microbiol. 2019;57(3):185-194. Published online February 26, 2019
-
DOI: https://doi.org/10.1007/s12275-019-8520-1
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77
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9
Web of Science
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7
Crossref
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Abstract
-
Tripartite efflux pumps and the type I secretion system of
Gram-negative bacteria are large protein complexes that span
the entire cell envelope. These complexes expel antibiotics and
other toxic substances or transport protein toxins from bacterial
cells. Elucidating the binary and ternary complex structures
at an atomic resolution are crucial to understanding the
assembly and working mechanism. Recent advances in cryoelectron
microscopy along with the construction of chimeric
proteins drastically shifted the assembly models. In this review,
we describe the current assembly models from a historical
perspective and emphasize the common assembly mechanism
for the assembly of diverse tripartite pumps and type
I secretion systems.
-
Citations
Citations to this article as recorded by - Molecular mechanisms of antibiotic resistance revisited
Elizabeth M. Darby, Eleftheria Trampari, Pauline Siasat, Maria Solsona Gaya, Ilyas Alav, Mark A. Webber, Jessica M. A. Blair
Nature Reviews Microbiology.2023; 21(5): 280. CrossRef - Permeation of Fosfomycin through the Phosphate-Specific Channels OprP and OprO of Pseudomonas aeruginosa
Vinaya Kumar Golla, Claudio Piselli, Ulrich Kleinekathöfer, Roland Benz
The Journal of Physical Chemistry B.2022; 126(7): 1388. CrossRef - Adaptation of the periplasm to maintain spatial constraints essential for cell envelope processes and cell viability
Eric Mandela, Christopher J Stubenrauch, David Ryoo, Hyea Hwang, Eli J Cohen, Von L Torres, Pankaj Deo, Chaille T Webb, Cheng Huang, Ralf B Schittenhelm, Morgan Beeby, JC Gumbart, Trevor Lithgow, Iain D Hay
eLife.2022;[Epub] CrossRef - Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria
Ilyas Alav, Jessica Kobylka, Miriam S. Kuth, Klaas M. Pos, Martin Picard, Jessica M. A. Blair, Vassiliy N. Bavro
Chemical Reviews.2021; 121(9): 5479. CrossRef - Biotechnological applications of type 1 secretion systems
Zohreh Pourhassan N., Sander H.J. Smits, Jung Hoon Ahn, Lutz Schmitt
Biotechnology Advances.2021; 53: 107864. CrossRef - Protein-Protein Interactions in the Cytoplasmic Membrane of Escherichia coli: Influence of the Overexpression of Diverse Transporter-Encoding Genes on the Activities of PTS Sugar Uptake Systems
Mohammad Aboulwafa, Zhongge Zhang, Milton H. Saier Jr.
Microbial Physiology.2020; 30(1-6): 36. CrossRef - Perspectives towards antibiotic resistance: from molecules to population
Joon-Hee Lee
Journal of Microbiology.2019; 57(3): 181. CrossRef
- Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase E inhibitor RraA protein family
-
Nohra Park , Jihune Heo , Saemee Song , Inseong Jo , Kangseok Lee , Nam-Chul Ha
-
J. Microbiol. 2017;55(5):388-395. Published online April 29, 2017
-
DOI: https://doi.org/10.1007/s12275-017-7053-8
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76
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0
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5
Crossref
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Abstract
-
Bacterial ribonuclease E (RNase E) plays a crucial role in the processing and decay of RNAs. A small protein named RraA negatively regulates the activity of RNase E via protein-protein interaction in various bacteria. Recently, RraAS1 and RraAS2, which are functional homologs of RraA from Escherichia coli, were identified in the Gram-positive species Streptomyces coelicolor. RraAS1 and RraAS2 inhibit RNase ES ribonuclease activity in S. coelicolor. RraAS1 and RraAS2 have a C-termi-nal extension region unlike typical bacterial RraA proteins. In this study, we present the crystal structure of RraAS2, ex-hibiting a hexamer arranged in a dimer of trimers, consistent with size exclusion chromatographic results. Importantly, the C-terminal extension region formed a long α-helix at the junction of the neighboring subunit, which is similar to the trimeric RraA orthologs from Saccharomyces cerevisiae. Trun-cation of the C-terminal extension region resulted in loss of RNase ES inhibition, demonstrating its crucial role. Our find-ings present the first bacterial RraA that has a hexameric assembly with a C-terminal extension α-helical region, which plays an essential role in the regulation of RNase ES activity in S. coelicolor.
-
Citations
Citations to this article as recorded by - Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2023; 61(2): 211. CrossRef - An oxidative metabolic pathway of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEHU) from alginate in an alginate-assimilating bacterium
Ryuji Nishiyama, Takao Ojima, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Akira Inoue
Communications Biology.2021;[Epub] CrossRef - The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
Scientific Reports.2019;[Epub] CrossRef - RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
Jaejin Lee, Dong-Ho Lee, Che Ok Jeon, Kangseok Lee
Journal of Microbiology.2019; 57(10): 910. CrossRef - Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus
Saemee Song, Seokho Hong, Jinyang Jang, Ji-Hyun Yeom, Nohra Park, Jaejin Lee, Yeri Lim, Jun-Yeong Jeon, Hyung-Kyoon Choi, Minho Lee, Nam-Chul Ha, Kangseok Lee, Eric Cascales
PLOS ONE.2017; 12(12): e0190064. CrossRef
- RraAS1 inhibits the ribonucleolytic activity of RNase ES by interacting with its catalytic domain in Streptomyces coelicolor
-
Sojin Seo , Daeyoung Kim , Wooseok Song , Jihune Heo , Minju Joo , Yeri Lim , Ji-Hyun Yeom , Kangseok Lee
-
J. Microbiol. 2017;55(1):37-43. Published online December 30, 2016
-
DOI: https://doi.org/10.1007/s12275-017-6518-0
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73
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0
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8
Crossref
-
Abstract
-
RraA is a protein inhibitor of RNase E, which degrades and
processes numerous RNAs in Escherichia coli. Streptomyces
coelicolor also contains homologs of RNase E and RraA,
RNase ES and RraAS1/RraAS2, respectively. Here, we report
that, unlike other RraA homologs, RraAS1 directly interacts
with the catalytic domain of RNase ES to exert its inhibitory
effect. We further show that rraAS1 gene deletion in S. coelicolor
results
in a higher growth rate and increased production
of actinorhodin and undecylprodigiosin, compared with
the wild-type strain, suggesting that RraAS1-mediated regulation
of RNase ES activity contributes to modulating the
cellular physiology of S. coelicolor.
-
Citations
Citations to this article as recorded by -
Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in
Vibrio alginolyticus
Huizhen Chen, Qian Gao, Bing Liu, Ying Zhang, Jianxiang Fang, Songbiao Wang, Youqi Chen, Chang Chen, Nicolas E. Buchler
mSphere.2024;[Epub] CrossRef - Streptomyces RNases – Function and impact on antibiotic synthesis
George H. Jones
Frontiers in Microbiology.2023;[Epub] CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef - The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
Scientific Reports.2019;[Epub] CrossRef - RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
Jaejin Lee, Dong-Ho Lee, Che Ok Jeon, Kangseok Lee
Journal of Microbiology.2019; 57(10): 910. CrossRef - Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus
Saemee Song, Seokho Hong, Jinyang Jang, Ji-Hyun Yeom, Nohra Park, Jaejin Lee, Yeri Lim, Jun-Yeong Jeon, Hyung-Kyoon Choi, Minho Lee, Nam-Chul Ha, Kangseok Lee, Eric Cascales
PLOS ONE.2017; 12(12): e0190064. CrossRef - Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase E inhibitor RraA protein family
Nohra Park, Jihune Heo, Saemee Song, Inseong Jo, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2017; 55(5): 388. CrossRef
- RraAS2 requires both scaffold domains of RNase ES for high-affinity binding and inhibitory action on the ribonucleolytic activity
-
Jihune Heo , Daeyoung Kim , Minju Joo , Boeun Lee , Sojin Seo , Jaejin Lee , Saemee Song , Ji-Hyun Yeom , Nam-Chul Ha , Kangseok Lee
-
J. Microbiol. 2016;54(10):660-666. Published online September 30, 2016
-
DOI: https://doi.org/10.1007/s12275-016-6417-9
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73
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0
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9
Crossref
-
Abstract
-
RraA is a protein inhibitor of RNase E (Rne), which catalyzes
the endoribonucleolytic cleavage of a large proportion
of RNAs in Escherichia coli. The antibiotic‐producing bacterium
Streptomyces coelicolor also contains homologs of
RNase E and RraA, designated as RNase ES (Rns), RraAS1,
and RraAS2, respectively. Here, we report that RraAS2 requires
both scaffold domains of RNase ES for high-affinity
binding and inhibitory action on the ribonucleolytic activity.
Analyses of the steady-state level of RNase E substrates indicated
that coexpression of RraAS2 in E. coli cells overproducing
Rns effectively inhibits the ribonucleolytic activity of
full-length RNase ES, but its inhibitory effects were moderate
or undetectable on other truncated forms of Rns, in which the
N- or/and C-terminal scaffold domain was deleted. In addition,
RraAS2 more efficiently inhibited the in vitro ribonucleolytic
activity of RNase ES than that of a truncated form
containing the catalytic domain only. Coimmunoprecipitation
and in vivo cross-linking experiments further showed
necessity of both scaffold domains of RNase ES for high-affinity
binding of RraAS2 to the enzyme, resulting in decreased
RNA-binding capacity of RNase ES. Our results indicate that
RraAS2 is a protein inhibitor of RNase ES and provide clues
to how this inhibitor affects the ribonucleolytic activity of
RNase ES.
-
Citations
Citations to this article as recorded by -
Identification of the global regulatory roles of RraA via the integrative transcriptome and proteome in
Vibrio alginolyticus
Huizhen Chen, Qian Gao, Bing Liu, Ying Zhang, Jianxiang Fang, Songbiao Wang, Youqi Chen, Chang Chen, Nicolas E. Buchler
mSphere.2024;[Epub] CrossRef - Streptomyces RNases – Function and impact on antibiotic synthesis
George H. Jones
Frontiers in Microbiology.2023;[Epub] CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef - Divergent rRNAs as regulators of gene expression at the ribosome level
Wooseok Song, Minju Joo, Ji-Hyun Yeom, Eunkyoung Shin, Minho Lee, Hyung-Kyoon Choi, Jihwan Hwang, Yong-In Kim, Ramin Seo, J. Eugene Lee, Christopher J. Moore, Yong-Hak Kim, Seong-il Eyun, Yoonsoo Hahn, Jeehyeon Bae, Kangseok Lee
Nature Microbiology.2019; 4(3): 515. CrossRef - RraAS1 inhibits the ribonucleolytic activity of RNase ES by interacting with its catalytic domain in Streptomyces coelicolor
Sojin Seo, Daeyoung Kim, Wooseok Song, Jihune Heo, Minju Joo, Yeri Lim, Ji-Hyun Yeom, Kangseok Lee
Journal of Microbiology.2017; 55(1): 37. CrossRef - Bdm-Mediated Regulation of Flagellar Biogenesis in Escherichia coli and Salmonella enterica Serovar Typhimurium
Jaejin Lee, Dae-Jun Kim, Ji-Hyun Yeom, Kangseok Lee
Current Microbiology.2017; 74(9): 1015. CrossRef - Functional implications of hexameric assembly of RraA proteins from Vibrio vulnificus
Saemee Song, Seokho Hong, Jinyang Jang, Ji-Hyun Yeom, Nohra Park, Jaejin Lee, Yeri Lim, Jun-Yeong Jeon, Hyung-Kyoon Choi, Minho Lee, Nam-Chul Ha, Kangseok Lee, Eric Cascales
PLOS ONE.2017; 12(12): e0190064. CrossRef - Crystal structure of Streptomyces coelicolor RraAS2, an unusual member of the RNase E inhibitor RraA protein family
Nohra Park, Jihune Heo, Saemee Song, Inseong Jo, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2017; 55(5): 388. CrossRef
- MINIREVIEW] Regulation of Escherichia coli RNase III activity
-
Boram Lim , Minji Sim , Howoon Lee , Seogang Hyun , Younghoon Lee , Yoonsoo Hahn , Eunkyoung Shin , Kangseok Lee
-
J. Microbiol. 2015;53(8):487-494. Published online July 31, 2015
-
DOI: https://doi.org/10.1007/s12275-015-5323-x
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101
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12
Crossref
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Abstract
-
Bacterial cells respond to changes in the environment by
adjusting their physiological reactions. In cascades of cellular
responses to stresses of various origins, rapid modulation
of RNA function is known to be an effective biochemical
adaptation. Among many factors affecting RNA function,
RNase III, a member of the phylogenetically highly conserved
endoribonuclease III family, plays a key role in posttranscriptional
regulatory pathways in Escherichia coli. In
this review, we provide an overview of the factors affecting
RNase III activity in E. coli.
-
Citations
Citations to this article as recorded by - Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant
Seungmok Han, Ji-Won Byun, Minho Lee
Journal of Microbiology.2024; 62(1): 33. CrossRef - arfAantisense RNA regulates MscL excretory activity
Rosa Morra, Fenryco Pratama, Thomas Butterfield, Geizecler Tomazetto, Kate Young, Ruth Lopez, Neil Dixon
Life Science Alliance.2023; 6(6): e202301954. CrossRef - Transcriptome and metabolome analyses of response of Synechocystis sp. PCC 6803 to methyl viologen
Xinyu Hu, Tianyuan Zhang, Kai Ji, Ke Luo, Li Wang, Wenli Chen
Applied Microbiology and Biotechnology.2021; 105(21-22): 8377. CrossRef - Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression
Minho Lee, Minkyung Ryu, Minju Joo, Young-Jin Seo, Jaejin Lee, Hong-Man Kim, Eunkyoung Shin, Ji-Hyun Yeom, Yong-Hak Kim, Jeehyeon Bae, Kangseok Lee, William Navarre
PLOS Pathogens.2021; 17(2): e1009263. CrossRef - Trans-acting regulators of ribonuclease activity
Jaejin Lee, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(4): 341. CrossRef - RNase III, Ribosome Biogenesis and Beyond
Maxence Lejars, Asaki Kobayashi, Eliane Hajnsdorf
Microorganisms.2021; 9(12): 2608. CrossRef - The rnc Gene Regulates the Microstructure of Exopolysaccharide in the Biofilm of Streptococcus mutans through the β-Monosaccharides
Yangyu Lu, Hongyu Zhang, Meng Li, Mengying Mao, Jiaqi Song, Yalan Deng, Lei Lei, Yingming Yang, Tao Hu
Caries Research.2021; 55(5): 534. CrossRef - Distributive enzyme binding controlled by local RNA context results in 3′ to 5′ directional processing of dicistronic tRNA precursors byEscherichia coliribonuclease P
Jing Zhao, Michael E Harris
Nucleic Acids Research.2019; 47(3): 1451. CrossRef - RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
Jaejin Lee, Dong-Ho Lee, Che Ok Jeon, Kangseok Lee
Journal of Microbiology.2019; 57(10): 910. CrossRef - The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
Minho Lee, Minju Joo, Minji Sim, Se-Hoon Sim, Hyun-Lee Kim, Jaejin Lee, Minkyung Ryu, Ji-Hyun Yeom, Yoonsoo Hahn, Nam-Chul Ha, Jang-Cheon Cho, Kangseok Lee
Scientific Reports.2019;[Epub] CrossRef - Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III inStreptococcus pyogenes
Anaïs Le Rhun, Anne-Laure Lécrivain, Johan Reimegård, Estelle Proux-Wéra, Laura Broglia, Cristina Della Beffa, Emmanuelle Charpentier
Nucleic Acids Research.2017; : gkw1316. CrossRef - Regulation and functions of bacterial PNPase
Federica Briani, Thomas Carzaniga, Gianni Dehò
WIREs RNA.2016; 7(2): 241. CrossRef
- MINIREVIEW] Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
-
Saemee Song , Jin-Sik Kim , Kangseok Lee , Nam-Chul Ha
-
J. Microbiol. 2015;53(6):355-364. Published online May 30, 2015
-
DOI: https://doi.org/10.1007/s12275-015-5248-4
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67
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9
Crossref
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Abstract
-
Gram-negative bacteria expel a wide range of toxic substances
through tripartite drug efflux pumps consisting of an inner
membrane transporter, an outer membrane channel protein,
and a periplasmic adaptor protein. These pumps form tripartite
assemblies which can span the entire cell envelope,
including the inner and outer membranes. There have been
controversial findings regarding the assembly of the individual
components in tripartite drug efflux pumps. Recent
structural and functional studies have advanced our understanding
of the assembly and working mechanisms of the
pumps. Here, we re-evaluate the assembly models based on
recent structural and functional studies. In particular, this
study focuses on the ‘adaptor bridging model’, highlighting
the intermeshing cogwheel-like interactions between the tip
regions of the outer membrane channel protein and the periplasmic
adaptor protein in the hexameric assembly.
-
Citations
Citations to this article as recorded by - Structural Features and Energetics of the Periplasmic Entrance Opening of the Outer Membrane Channel TolC Revealed by Molecular Dynamics Simulation and Markov State Model Analysis
Jingwei Weng, Wenning Wang
Journal of Chemical Information and Modeling.2019; 59(5): 2359. CrossRef - Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
Inseong Jo, Jin-Sik Kim, Yongbin Xu, Jaekyung Hyun, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2019; 57(3): 185. CrossRef - Antibiotic Hybrids: the Next Generation of Agents and Adjuvants against Gram-Negative Pathogens?
Ronald Domalaon, Temilolu Idowu, George G. Zhanel, Frank Schweizer
Clinical Microbiology Reviews.2018;[Epub] CrossRef - Genetic identification of factors for extracellular cellulose accumulation in the thermophilic cyanobacterium Thermosynechococcus vulcanus: proposal of a novel tripartite secretion system
Kaisei Maeda, Jyunya Tamura, Yukiko Okuda, Rei Narikawa, Takafumi Midorikawa, Masahiko Ikeuchi
Molecular Microbiology.2018; 109(1): 121. CrossRef - Switch Loop Flexibility Affects Substrate Transport of the AcrB Efflux Pump
Reinke T. Müller, Timothy Travers, Hi-jea Cha, Joshua L. Phillips, S. Gnanakaran, Klaas M. Pos
Journal of Molecular Biology.2017; 429(24): 3863. CrossRef - Molecular Rationale behind the Differential Substrate Specificity of Bacterial RND Multi-Drug Transporters
Venkata Krishnan Ramaswamy, Attilio V. Vargiu, Giuliano Malloci, Jürg Dreier, Paolo Ruggerone
Scientific Reports.2017;[Epub] CrossRef - Structure of the MacAB–TolC ABC-type tripartite multidrug efflux pump
Anthony W. P. Fitzpatrick, Salomé Llabrés, Arthur Neuberger, James N. Blaza, Xiao-Chen Bai, Ui Okada, Satoshi Murakami, Hendrik W. van Veen, Ulrich Zachariae, Sjors H. W. Scheres, Ben F. Luisi, Dijun Du
Nature Microbiology.2017;[Epub] CrossRef - Structural Basis for the Serratia marcescens Lipase Secretion System: Crystal Structures of the Membrane Fusion Protein and Nucleotide-Binding Domain
Daichi Murata, Hiroyuki Okano, Clement Angkawidjaja, Masato Akutsu, Shun-ichi Tanaka, Kenyu Kitahara, Takuya Yoshizawa, Hiroyoshi Matsumura, Yuji Kado, Eiichi Mizohata, Tsuyoshi Inoue, Satoshi Sano, Yuichi Koga, Shigenori Kanaya, Kazufumi Takano
Biochemistry.2017; 56(47): 6281. CrossRef - The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens
Yongbin Xu, Jianyun Guo, Lulu Wang, Rui Jiang, Xiaoling Jin, Jing Liu, Shengdi Fan, Chun-Shan Quan, Nam-Chul Ha, Bostjan Kobe
PLOS ONE.2016; 11(5): e0155846. CrossRef
- Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB
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Seunghwa Lee , Ji-Hyun Yeom , Sojin Seo , Minho Lee , Sarang Kim , Jeehyeon Bae , Kangseok Lee , Jihwan Hwang
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J. Microbiol. 2015;53(4):256-261. Published online March 4, 2015
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DOI: https://doi.org/10.1007/s12275-015-5037-0
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85
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10
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Abstract
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Resistance-nodulation-division (RND) efflux pumps are associated
with multidrug resistance in many gram-negative
pathogens. The genome of Vibrio vulnificus encodes 11 putative
RND pumps homologous to those of Vibrio cholerae
and Escherichia coli. In this study, we analyzed three putative
RND efflux pumps, showing homology to V. cholerae VexAB
and VexCD and to E. coli AcrAB, for their functional roles in
multidrug resistance of V. vulnificus. Deletion of the vexAB
homolog resulted in increased susceptibility of V. vulnificus
to bile acid, acriflavine, ethidium bromide, and erythromycin,
whereas deletion of acrAB homologs rendered V. vulnificus
more susceptible to acriflavine only. Deletion of vexCD had
no effect on susceptibility of V. vulnificus to these chemicals.
Upon exposure to these antibacterial chemicals, expression
of tolCV1 and tolCV2, which are putative outer membrane
factors of RND efflux pumps, was induced, whereas expression
levels of vexAB, vexCD, and acrAB homologs were not
significantly changed. Our results show that the V. vulnificus
homologs of VexAB largely contributed to in vitro antimicrobial
resistance with a broad substrate specificity that
was partially redundant with the AcrAB pump homologs.
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Citations
Citations to this article as recorded by - Bile compounds are effective inducer of efflux pump-mediated antimicrobial resistance among Gram-negative bacteria
Hanieh Akbari, Robab Azargun, Mina Yekani, Somayeh Ahmadi, Hadi Ghanbari, Mohammad Yousef Memar, Tahereh Pirzadeh
Biologia.2025;[Epub] CrossRef - Dynamics of efflux pumps in antimicrobial resistance, persistence, and community living of Vibrionaceae
Sanath Kumar, Manjusha Lekshmi, Jerusha Stephen, Anely Ortiz-Alegria, Matthew Ayitah, Manuel F. Varela
Archives of Microbiology.2024;[Epub] CrossRef - Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence
Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
Microorganisms.2022; 10(2): 382. CrossRef - TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
Yue Gong, Rui Hong Guo, Joon Haeng Rhee, Young Ran Kim
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - The mechanisms that regulate Vibrio parahaemolyticus virulence gene expression differ between pathotypes
Nicholas Petronella, Jennifer Ronholm
Microbial Genomics
.2018;[Epub] CrossRef - ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
Wen-Jung Lu, Hsuan-Ju Lin, Thamarai Janganan, Cheng-Yi Li, Wei-Chiang Chin, Vassiliy Bavro, Hong-Ting Lin
International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef - Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus
Chung-Cheng Lo, Pei-Ting Lin, Chuan Chiang-Ni, Kuan-Hua Lin, Shin-Luen Lee, Tzu-Fang Kuo, Horng-Ren Lo
Gene Reports.2017; 9: 115. CrossRef - Survival of the Fittest: How Bacterial Pathogens Utilize Bile To Enhance Infection
Jeticia R. Sistrunk, Kourtney P. Nickerson, Rachael B. Chanin, David A. Rasko, Christina S. Faherty
Clinical Microbiology Reviews.2016; 29(4): 819. CrossRef - MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium
Saemee Song, Boeun Lee, Ji-Hyun Yeom, Soonhye Hwang, Ilnam Kang, Jang-Cheon Cho, Nam-Chul Ha, Jeehyeon Bae, Kangseok Lee, Yong-Hak Kim, S. M. Payne
Infection and Immunity.2015; 83(11): 4266. CrossRef - Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
Saemee Song, Jin-Sik Kim, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2015; 53(6): 355. CrossRef
- Interaction between the α-Barrel Tip of Vibrio vulnificus TolC Homologs and AcrA Implies the Adapter Bridging Model
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Seunghwa Lee , Saemee Song , Minho Lee , Soonhye Hwang , Ji-Sun Kim , Nam-Chul Ha , Kangseok Lee
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J. Microbiol. 2014;52(2):148-153. Published online February 1, 2014
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DOI: https://doi.org/10.1007/s12275-014-3578-2
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69
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0
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10
Crossref
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Abstract
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The AcrAB-TolC multidrug efflux pump confers resistance to Escherichia coli against many antibiotics and toxic compounds. The TolC protein is an outer membrane factor that participates in the formation of type I secretion systems. The genome of Vibrio vulnificus encodes two proteins homologous to the E. coli TolC, designated TolCV1 and TolCV2. Here, we show that both TolCV1 and TolCV2 partially complement the E. coli TolC function and physically interact with the membrane fusion protein AcrA, a component of the E. coli AcrAB-TolC efflux pump. Using site-directed mutational analyses and an in vivo cross-linking assay, we demonstrated that the α-barrel tip region of TolC homologs plays a critical role in the formation of functional AcrAB-TolC efflux pumps. Our findings suggest the adapter bridging model as a general assembly mechanism for tripartite drug efflux pumps in Gram-negative bacteria.
-
Citations
Citations to this article as recorded by - Progress of Antimicrobial Mechanisms of Stilbenoids
Xiancai Li, Yongqing Li, Binghong Xiong, Shengxiang Qiu
Pharmaceutics.2024; 16(5): 663. CrossRef - Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence
Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
Microorganisms.2022; 10(2): 382. CrossRef - TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
Yue Gong, Rui Hong Guo, Joon Haeng Rhee, Young Ran Kim
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Recent paradigm shift in the assembly of bacterial tripartite efflux pumps and the type I secretion system
Inseong Jo, Jin-Sik Kim, Yongbin Xu, Jaekyung Hyun, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2019; 57(3): 185. CrossRef - NaCl promotes antibiotic resistance by reducing redox states in Vibrio alginolyticus
Jun Yang, Zao‐Hai Zeng, Man‐Jun Yang, Zhi‐Xue Cheng, Xuan‐Xian Peng, Hui Li
Environmental Microbiology.2018; 20(11): 4022. CrossRef - ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
Wen-Jung Lu, Hsuan-Ju Lin, Thamarai Janganan, Cheng-Yi Li, Wei-Chiang Chin, Vassiliy Bavro, Hong-Ting Lin
International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef - Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB
Seunghwa Lee, Ji-Hyun Yeom, Sojin Seo, Minho Lee, Sarang Kim, Jeehyeon Bae, Kangseok Lee, Jihwan Hwang
Journal of Microbiology.2015; 53(4): 256. CrossRef - Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
Saemee Song, Jin-Sik Kim, Kangseok Lee, Nam-Chul Ha
Journal of Microbiology.2015; 53(6): 355. CrossRef - Interaction Mediated by the Putative Tip Regions of MdsA and MdsC in the Formation of a Salmonella-Specific Tripartite Efflux Pump
Saemee Song, Soonhye Hwang, Seunghwa Lee, Nam-Chul Ha, Kangseok Lee, Eric Cascales
PLoS ONE.2014; 9(6): e100881. CrossRef - Functional Analysis of TolC Homologs in Vibrio vulnificus
Seunghwa Lee, Saemee Song, Kangseok Lee
Current Microbiology.2014; 68(6): 729. CrossRef
- The α-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC
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Minho Lee , Hyun-Lee Kim , Saemee Song , Minju Joo , Seunghwa Lee , Daeyoung Kim , Yoonsoo Hahn , Nam-Chul Ha , Kangseok Lee
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J. Microbiol. 2013;51(2):154-159. Published online April 27, 2013
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DOI: https://doi.org/10.1007/s12275-013-2699-3
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57
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18
Scopus
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Abstract
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TolC and its homologous family of proteins are outer membrane factors that are essential for exporting small molecules and toxins across the outer membrane in Gram-negative bacteria. Two open reading frames in the Vibrio vulnificus genome that encode proteins homologous to Escherichia coli TolC, designated TolCV1 and TolCV2, have 51.3% and 29.6% amino acid identity to TolC, respectively. In this study, we show that TolCV1 and TolCV2 functionally and physically interacted with the membrane fusion protein, MacA, a component of the macrolide-specific MacAB-TolC pump of E. coli. We further show that the conserved residues located at the aperture tip region of the α-hairpin of TolCV1 and TolCV2 played an essential role in the formation of the functional MacAB-TolC pump using site-directed mutational analyses. Our findings suggest that these outer membrane factors have
conserved tip-to-tip interaction with the MacA membrane fusion protein for action of the drug efflux pump in Gramnegative bacteria.
- RNase G Participates in Processing of the 5′-end of 23S Ribosomal RNA
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Woo-Seok Song , Minho Lee , Kangseok Lee
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J. Microbiol. 2011;49(3):508-511. Published online June 30, 2011
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DOI: https://doi.org/10.1007/s12275-011-1198-7
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39
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0
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7
Scopus
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Abstract
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In Escherichia coli, primary rRNA transcripts must be processed by a complex process in which several ribonucleases are involved in order to generate mature 16S, 23S, and 5S rRNA molecules. While it is known that RNase G, a single-stranded RNA-specific endoribonuclease encoded by the rng gene, plays an active role in the maturation of the 5′-end of 16S rRNA, its involvement in the maturation of the 5′-end of 23S rRNA remains unclear. Here we show that E. coli cells deleted for the rng gene accumulate the 23S rRNA precursor containing an extra 77 nucleotides at its mature 5′-end. In vitro cleavage assays show that RNase G cleaves synthetic RNA containing a sequence encompassing the 5′-end to 77 nucleotides upstream of mature 23S rRNA at two sites present in single-stranded regions. Our results suggest the involvement of RNase G in the processing of the 5′-region of 23S rRNA precursors.
- Periplasmic Domain of CusA in an Escherichia coli Cu+/Ag+ Transporter Has Metal Binding Sites
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Bo-Young Yun , Yongbin Xu , Shunfu Piao , Nahee Kim , Jeong-Hyun Yoon , Hyun-Soo Cho , Kangseok Lee , Nam-Chul Ha
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J. Microbiol. 2010;48(6):829-835. Published online January 9, 2011
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DOI: https://doi.org/10.1007/s12275-010-0339-8
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40
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0
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6
Scopus
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Abstract
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The resistance nodulation division (RND)-type efflux systems are utilized in Gram-negative bacteria to export a variety of substrates. The CusCFBA system is the Cu+ and Ag+ efflux system in Escherichia coli, conferring resistance to lethal concentrations of Cu+ and Ag+. The periplasmic component, CusB, which is
essential for the assembly of the protein complex, has Cu+ or Ag+ binding sites. The twelve-span membrane protein CusA is a homotrimeric transporter, and has a relatively large periplasmic domain. Here, we constructed the periplasmic domain of CusA by joining two DNA segments and then successfully expressed and purified the protein. Isothermal titration calorimetry experiments revealed Ag+ binding sites with Kds of 10-6-10-5 M. Our findings suggest that the metal binding in the periplasmic domain of CusA might play an important role in the function of the efflux pump.
- Functional Analysis of the Invariant Residue G791 of Escherichia coli 16S rRNA
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Woo-Seok Song , Hong-Man Kim , Jae-Hong Kim , Se-Hoon Sim , Sang-Mi Ryou , Sanggoo Kim , Chang-Jun Cha , Philip R. Cunningham , Jeehyeon Bae , Kangseok Lee
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J. Microbiol. 2007;45(5):418-421.
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DOI: https://doi.org/2595 [pii]
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Abstract
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The nucleotide at position 791(G791) of E. coli 16S rRNA was previously identified as an invariant residue for ribosomal function. In order to characterize the functional role of G791, base substitutions were introduced at this position, and mutant ribosomes were analyzed with regard to their protein synthesis ability, via the use of a specialized ribosome system. These ribosomal RNA mutations attenuated the ability of ribosomes to conduct protein synthesis by more than 65%. A transition mutation (G to A) exerted a moderate effect on ribosomal function, whereas a transversion mutation (G to C or U) resulted in a loss of protein synthesis ability of more than 90%. The sucrose gradient profiles of ribosomes and primer extension analysis showed that the loss of protein-synthesis ability of mutant ribosomes harboring a base substitution from G to U at position 791 stems partially from its inability to form 70S ribosomes. These findings show the involvement of the nucleotide at position 791 in the association of ribosomal subunits and protein synthesis steps after 70S formation, as well as the possibility of using 16S rRNA mutated at position 791 for the selection of second-site revertants in order to identify ligands that interact with G791 in protein synthesis.
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