Journal of Microbiology

Kang-Mu Lee

2 Articles

Structural and Functional Importance of Outer Membrane Proteins in Vibrio cholerae Flagellum: Wasimul Bari , Kang-Mu Lee , Sang Sun Yoon; J. Microbiol. 2012;50(4):631-637. Published online August 25, 2012; DOI: https://doi.org/10.1007/s12275-012-2116-3

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Vibrio cholerae has a sheath-covered monotrichous flagellum that is known to contribute to virulence. Although the structural organization of the V. cholerae flagellum has been extensively studied, the involvement of outer membrane proteins as integral components in the flagellum still remains elusive. Here we show that flagella produced by V. cholerae O1 El Tor strain C6706 were two times thicker than those from two other Gram-negative bacteria. A C6706 mutant strain (SSY11) devoid of two outer membrane proteins (OMPs), OmpU and OmpT, produced thinner flagella. SSY11 showed significant defects in the flagella-mediated motility as compared to its parental strain. Moreover, increased shedding of the flagella-associated proteins was observed in the culture supernatant of SSY11. This finding was also supported by the observation that culture supernatants of the SSY11 strain induced the production of a significantly higher level of IL-8 in human colon carcinoma HT29 and alveolar epithelial A549 cells than those of the wild-type C6706 strain. These results further suggest a definite role of these two OMPs in providing the structural integrity of the V. cholerae flagellum as part of the surrounding sheath.

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Functional Versatility of Vibrio cholerae Outer Membrane Proteins
Annabelle Mathieu-Denoncourt, Marylise Duperthuy
Applied Microbiology.2025; 5(3): 64. CrossRef
Outer membrane protein of OmpF contributes to swimming motility, biofilm formation, osmotic response as well as the transcription of maltose metabolic genes in Citrobacter werkmanii
Gang Zhou, Ying-Si Wang, Hong Peng, Hui-Zhong Liu, Jin Feng, Su-Juan Li, Ting-Li Sun, Cai-Ling Li, Qing-Shan Shi, Xiao-bao Xie
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef
Non-O1/O139 Vibrio cholerae causes severe intestinal disease in bullfrogs (Rana catesbeiana)
Wenyu Liao, Dongdong Wei, Mingzhu Liu, Ke Ke, Deqiang Shi, Bingzheng Li, Shuaishuai Huang, Jianbo Jiang, Qing Yu, Pengfei Li
Animal Diseases.2023;[Epub] CrossRef
Experimental evolution of Vibrio cholerae identifies hypervesiculation as a way to increase motility in the presence of polymyxin B
Sean Giacomucci, Annabelle Mathieu-Denoncourt, Antony T. Vincent, Hanen Jannadi, Marylise Duperthuy
Frontiers in Microbiology.2022;[Epub] CrossRef
Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
Chun-Hsing Liao, Chia-Lun Chang, Hsin-Hui Huang, Yi-Tsung Lin, Li-Hua Li, Tsuey-Ching Yang
Microorganisms.2021; 9(6): 1216. CrossRef
Phylogenetic Distribution, Ultrastructure, and Function of Bacterial Flagellar Sheaths
Joshua Chu, Jun Liu, Timothy R. Hoover
Biomolecules.2020; 10(3): 363. CrossRef
Maltoporin (LamB protein) contributes to the virulence and adhesion of Aeromonas veronii TH0426
Bintong Yang, Dongxing Zhang, Tonglei Wu, Zhiqiang Zhang, Sayed Haidar Abbas Raza, Nicola Schreurs, Lei Zhang, Guilian Yang, Chunfeng Wang, Aidong Qian, Yuanhuan Kang, Xiaofeng Shan
Journal of Fish Diseases.2019; 42(3): 379. CrossRef
Abundance and observations of thermophilic microbial and viral communities in submarine and terrestrial hot fluid systems of the French Southern and Antarctic Lands
Kaarle J. Parikka, Stéphan Jacquet, Jonathan Colombet, Damien Guillaume, Marc Le Romancer
Polar Biology.2018; 41(7): 1335. CrossRef
A proteomic analysis of the iron response of Photobacterium damselae subsp. damselae reveals metabolic adaptations to iron levels changes and novel potential virulence factors
Beatriz Puentes, Miguel Balado, José Bermúdez-Crespo, Carlos R. Osorio, Manuel L. Lemos
Veterinary Microbiology.2017; 201: 257. CrossRef
(p)ppGpp, a Small Nucleotide Regulator, Directs the Metabolic Fate of Glucose in Vibrio cholerae
Young Taek Oh, Kang-Mu Lee, Wasimul Bari, David M. Raskin, Sang Sun Yoon
Journal of Biological Chemistry.2015; 290(21): 13178. CrossRef
Outer membrane vesicles are vehicles for the delivery of Vibrio tasmaniensis virulence factors to oyster immune cells
Audrey Sophie Vanhove, Marylise Duperthuy, Guillaume M. Charrière, Frédérique Le Roux, David Goudenège, Benjamin Gourbal, Sylvie Kieffer‐Jaquinod, Yohann Couté, Sun Nyunt Wai, Delphine Destoumieux‐Garzón
Environmental Microbiology.2015; 17(4): 1152. CrossRef
Cholera Toxin Production during Anaerobic Trimethylamine N-Oxide Respiration Is Mediated by Stringent Response in Vibrio cholerae
Young Taek Oh, Yongjin Park, Mi Young Yoon, Wasimul Bari, Junhyeok Go, Kyung Bae Min, David M. Raskin, Kang-Mu Lee, Sang Sun Yoon
Journal of Biological Chemistry.2014; 289(19): 13232. CrossRef
Protective role of gut commensal microbes against intestinal infections
My Young Yoon, Keehoon Lee, Sang Sun Yoon
Journal of Microbiology.2014; 52(12): 983. CrossRef

Heterogeneous Virulence Potential and High Antibiotic Resistance of Pseudomonas aeruginosa Strains Isolated from Korean Pneumonia Patients: Mi Young Yoon , Kang-Mu Lee , Seok Hoon Jeong , Jungmin Kim , Sang Sun Yoon; J. Microbiol. 2010;48(4):518-525. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-9388-2

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Abstract PDF

Pseudomonas aeruginosa is an opportunistic human pathogen of clinical importance that causes airway infections in immunocompromised patients. Here, we report the virulence-associated characteristics of strains of P. aeruginosa, isolated from the sputa of 25 Korean pneumonia patients. A high degree of genomic plasticity was observed by random amplified polymorphic DNA genotype analysis, suggesting that the infections were caused by strains with diverse genomic backgrounds. Biofilm formation of each isolate was heterogeneous in terms of their relative motilities. In addition, 48% of isolates were defective in the production of 3-oxo-C12-HSL (PAI-1), a quorum sensing signal molecule. In these strains, PAI-1-dependent elastase production was correspondingly decreased, suggesting that a large number of strains were presumed to be quorum sensing deficient. Multidrug resistance (MDR) was seen in 56% of the isolates tested, and 44% of the MDR strains were resistant to five or more antibiotics. Taken together, our results provide additional insights into the virulence traits of P. aeruginosa clinical isolates, which will aid in treating P. aeruginosa infections in pneumonia patients.

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Virulence Characteristics and an Action Mode of Antibiotic Resistance in Multidrug-Resistant Pseudomonas aeruginosa
Wontae Hwang, Sang Sun Yoon
Scientific Reports.2019;[Epub] CrossRef
The ferrichrome receptor A as a new target forPseudomonas aeruginosavirulence attenuation
Keehoon Lee, Kang-Mu Lee, Junhyeok Go, Jae-Chan Ryu, Ji-Hwan Ryu, Sang Sun Yoon, Klaus Hantke
FEMS Microbiology Letters.2016; 363(11): fnw104. CrossRef
A single gene of a commensal microbe affects host susceptibility to enteric infection
Mi Young Yoon, Kyung Bae Min, Kang-Mu Lee, Yujin Yoon, Yaeseul Kim, Young Taek Oh, Keehoon Lee, Jongsik Chun, Byung-Yong Kim, Seok-Hwan Yoon, Insuk Lee, Chan Yeong Kim, Sang Sun Yoon
Nature Communications.2016;[Epub] CrossRef
Anaerobiosis-Induced Loss of Cytotoxicity Is Due to Inactivation of Quorum Sensing in Pseudomonas aeruginosa
Kang-Mu Lee, Mi Young Yoon, Yongjin Park, Joon-Hee Lee, Sang Sun Yoon, B. A. McCormick
Infection and Immunity.2011; 79(7): 2792. CrossRef

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