- Therapeutic potential of an AcHERV-HPV L1 DNA vaccine
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Hee-Jung Lee , Jong Kwang Yoon , Yoonki Heo , Hansam Cho , Yeondong Cho , Yongdae Gwon , Kang Chang Kim , Jiwon Choi , Jae Sung Lee , Yu-Kyoung Oh , Young Bong Kim
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J. Microbiol. 2015;53(6):415-420. Published online May 30, 2015
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DOI: https://doi.org/10.1007/s12275-015-5150-0
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Cervical cancer is strongly associated with chronic human
papillomavirus infections, among which HPV16 is the most
common. Two commercial HPV vaccines, Gardasil and
Cervarix are effective for preventing HPV infection, but cannot
be used to treat existing HPV infections. Previously, we
developed a human endogenous retrovirus (HERV)-enveloped
recombinant baculovirus capable of delivering the L1
genes of HPV types 16, 18, and 58 (AcHERV-HP16/18/58L1,
AcHERV-HPV). Intramuscular administration of AcHERVHPV
vaccines induced a strong cellular immune response
as well as a humoral immune response. In this study, to examine
the therapeutic effect of AcHERV-HPV in a mouse
model, we established an HPV16 L1 expressing tumor cell
line. Compared to Cervarix, immunization with AcHERVHPV
greatly enhanced HPV16 L1-specific cytotoxic T lymphocytes
(CTL) in C57BL/6 mice. Although vaccination
could not remove preexisting tumors, strong CTL activity
retarded the growth of inoculated tumor cells. These results
indicate that AcHERV-HPV could serve as a potential therapeutic
DNA vaccine against concurrent infection with HPV
16, 18, and 58.
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Citations
Citations to this article as recorded by  - Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia
Chichao Xia, Sile Li, Teng Long, Zigui Chen, Paul K. S. Chan, Siaw Shi Boon
Cancers.2021; 13(11): 2691. CrossRef - In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses
Matin Kayyal, Azam Bolhassani, Zahra Noormohammadi, Majid Sadeghizadeh
Molecular Biotechnology.2021; 63(12): 1192. CrossRef - Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery
Ha Youn Shin, Hanul Choi, Nahyun Kim, Nayoung Park, Heesun Kim, Jaebum Kim, Young Bong Kim
Genes.2020; 11(11): 1306. CrossRef - Treatment with Radix Euphorbiae Ebracteolatae Significantly Decreases the Expression of E6 and L1, and Increases the Expression of p53 and Rb in HPV18-infected Human Foreskin Keratinocytes
Xiang He, Xufeng He, Ping Xu, Lili Yang, Xin Ma, Wen Li, Huimin Zhang
Current Molecular Medicine.2019; 19(1): 20. CrossRef - Trial watch: DNA-based vaccines for oncological indications
Stefano Pierini, Renzo Perales-Linares, Mireia Uribe-Herranz, Jonathan G. Pol, Laurence Zitvogel, Guido Kroemer, Andrea Facciabene, Lorenzo Galluzzi
OncoImmunology.2017; 6(12): e1398878. CrossRef - Broad-spectrum antiviral properties of andrographolide
Swati Gupta, K. P. Mishra, Lilly Ganju
Archives of Virology.2017; 162(3): 611. CrossRef
- Identification of Porcine Endogenous Retrovirus (PERV) packaging sequence and development of PERV packaging viral vector system
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Jiwon Choi , Hoon-mi Kim , Jong Kwang Yoon , Yeondong Cho , Hee-Jung Lee , Kang Chang Kim , Chang-Kyu Kim , Gye-Woong Kim , Young Bong Kim
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J. Microbiol. 2015;53(5):348-353. Published online May 3, 2015
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DOI: https://doi.org/10.1007/s12275-015-5134-0
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Abstract
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Studies of the retroviruses have focused on the specific interaction
of the nucleocapsid protein with a packaging signal
in the viral RNA as important for this selectivity, but the
packaging signal in porcine endogenous retrovirus (PERV)
has not been defined. Herein, we identified and analyzed
this packaging signal in PERV and found hairpin structures
with conserved tetranucleotides in their loops and nucleocapsid
recognition sequences; both of which are key elements
in the viral packaging signal of MLV. We evaluated packaging
efficiency of sequence variants isolated from viral and
proviral integrated genomes. All viral packaging sequences
(Ψ) were identical, while five distinct packaging sequences
were identified from proviral sources. One proviral sequence
(Ψ1) was identical to that of the viral Ψ and had the highest
packaging efficiency. Three variants (Ψ2, Ψ3, Ψ4) maintained
key elements of the viral packaging signal, but had nucleotide
replacements and consequently demonstrated reduced
packaging efficiency. Despite of the same overall hairpin
structure, the proviral variant (Ψ5) had only one GACG sequence
in the hairpin loop and showed the lowest packaging
efficiency other than ΔΨ, in which the essential packaging
sequence was removed. This result, thus, defined the
packaging sequences in PERV and emphasized the importance
of nucleotide sequence and RNA structure in the determination
of packaging efficiency. In addition, we demonstrate
efficient infection and gene expression from the PERVbased
viral vector, which may serve as a novel alternative to
current retroviral expression systems.
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Citations
Citations to this article as recorded by - Characterization of porcine endogenous retrovirus insertion in Jeju native pigs and commercial breeds
Seungwon Yoon, Mrinmoy Ghosh, Myeongyeon Shin, Hyunyong Choi, Cheol-Ho Hyun, Dae Cheol Kim, Shin Ji Lee, Min Jee An, Young-Ok Son, Chang-Gi Hur
Animal Bioscience.2025; 38(12): 2741. CrossRef - Porcine Endogenous Retrovirus (PERV) – Molecular Structure and Replication Strategy in the Context of Retroviral Infection Risk of Human Cells
Krzysztof Łopata, Emilia Wojdas, Roman Nowak, Paweł Łopata, Urszula Mazurek
Frontiers in Microbiology.2018;[Epub] CrossRef
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