- Pneumococcal wall teichoic acid is required for the pathogenesis of Streptococcus pneumoniae in murine models
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Hongmei Xu , Libin Wang , Jian Huang , Yanqing Zhang , Feng Ma , Jianmin Wang , Wenchun Xu , Xuemei Zhang , Yibing Yin , Kaifeng Wu
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J. Microbiol. 2015;53(2):147-154. Published online January 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-4616-4
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Pneumococcal asymptomatic colonization of the respiratory
tracts is a major risk for invasive pneumococcal disease.
We have previously shown that pneumococcal wall teichoic
acid (WTA) was involved in pneumococcal infection of sepsis
and adherence to epithelial and endothelial cells. In this
study, we investigated the contribution of pneumococcal
WTA to bacterial colonization and dissemination in murine
models. The result showed that nasopharynx colonizing D39
bacterial cells have a distinct phenotype showing an increased
exposure of teichoic acids relative to medium-grown bacteria.
The WTA-deficient mutants were impaired in their colonization
to the nasopharynx and lungs, and led to a mild inflammation
in the lungs at 36 h post-inoculation. Pretreatment
of the murine nares with WTA reduced the ability of
wild type D39 bacteria to colonize the nasopharynx. In addition,
the WTA-deficient strain was impaired in its ability
to invade the blood and brain following intranasal administration.
WTA-deficient D39 strain was reduced in C3 deposition
but was more susceptible to the killing by the neutrophils
as compared with its parent strain. Our results also
demonstrated that the WTA enhanced pneumococcal colonization
and dissemination independently of the host strains.
These results indicate that WTA plays an important role in
pneumococcal pathogenesis, both in colonization and dissemination
processes.
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Citations
Citations to this article as recorded by  - Insight into the structure, biosynthesis, isolation method and biological function of teichoic acid in different gram-positive microorganisms: A review
Jiarun Han, Xin Zhao, Xilian Zhao, Ping Li, Qing Gu
International Journal of Biological Macromolecules.2023; 253: 126825. CrossRef - spd1672, a novel in vivo-induced gene, affects inflammatory response in a murine model of Streptococcus pneumoniae infection
Lingling Gan, Xuemei Zhang, Xiuyu Xu, Wenchun Xu, Chang Lu, Jin Cui, Hong Wang
Canadian Journal of Microbiology.2018; 64(6): 401. CrossRef - Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
Nathalie Heß, Franziska Waldow, Thomas P. Kohler, Manfred Rohde, Bernd Kreikemeyer, Alejandro Gómez-Mejia, Torsten Hain, Dominik Schwudke, Waldemar Vollmer, Sven Hammerschmidt, Nicolas Gisch
Nature Communications.2017;[Epub] CrossRef - New chemical tools to probe cell wall biosynthesis in bacteria
Robert T Gale, Eric D Brown
Current Opinion in Microbiology.2015; 27: 69. CrossRef
- Serotype-Independent Protection against Pneumococcal Infections Elicited by Intranasal Immunization with Ethanol-Killed Pneumococcal Strain, SPY1
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Xiuyu Xu , Jiangping Meng , Yiping Wang , Jie Zheng , Kaifeng Wu , Xuemei Zhang , Yibing Yin , Qun Zhang
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J. Microbiol. 2014;52(4):315-323. Published online March 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-3583-5
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1,017
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The 23-valent polysaccharide vaccine and the 7-valent pneumococcal conjugate vaccine are licensed vaccines that protect against pneumococcal infections worldwide. However, the incidence of pneumococcal diseases remains high in lowincome countries. Whole-cell vaccines with high safety and strong immunogenicity may be a favorable choice. We previously obtained a capsule-deficient Streptococcus pneumoniae mutant named SPY1 derived from strain D39. As an attenuated live pneumococcal vaccine, intranasal immunization with SPY1 elicits broad serotype-independent protection against pneumococcal infection. In this study, for safety consideration, we inactivated SPY1 with 70% ethanol and intranasally immunized BALB/c mice with killed SPY1 plus cholera toxin adjuvant for four times. Results showed that intranasal immunization with inactivated SPY1 induced strong humoral and cellular immune responses. Intranasal immunization with inactivated SPY1 plus cholera toxin adjuvant elicited effective serotype-independent protection against the colonization of pneumococcal strains 19F and 4 as well as lethal infection of pneumococcal serotypes 2, 3, 14, and 6B.
The protection rates provided by inactivated SPY1 against lethal pneumococcal infection were comparable to those of currently used polysaccharide vaccines. In addition, vaccinespecific B-cell and T-cell immune responses mediated the
protection elicited by SPY1. In conclusion, the 70% ethanolinactivated
pneumococcal whole-cell vaccine SPY1 is a potentially safe and less complex vaccine strategy that offers broad protection against S. pneumoniae.
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Citations
Citations to this article as recorded by - Pneumococcal live attenuated vaccine: strategies, challenges and future perspectives
Marina Yusoff, Chew Chieng Yeo, Malik Amonov
Exploration of Immunology.2026;[Epub] CrossRef -
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Human Vaccines & Immunotherapeutics.2025;[Epub] CrossRef - Otitis media: recent advances in otitis media vaccine development and model systems
Ayesha Zahid, Jennifer C. Wilson, I. Darren Grice, Ian R. Peak
Frontiers in Microbiology.2024;[Epub] CrossRef - Corrected and Republished from: “A Novel, Multiple-Antigen Pneumococcal Vaccine Protects against LethalStreptococcus pneumoniaeChallenge”
Win-Yan Chan, Claire Entwisle, Giuseppe Ercoli, Elise Ramos-Sevillano, Ann McIlgorm, Paola Cecchini, Christopher Bailey, Oliver Lam, Gail Whiting, Nicola Green, David Goldblatt, Jun X. Wheeler, Jeremy S. Brown, Liise-anne Pirofski
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Pedro H. Silva, Yaneisi Vázquez, Camilo Campusano, Angello Retamal-Díaz, Margarita K. Lay, Christian A. Muñoz, Pablo A. González, Alexis M. Kalergis, Susan M. Bueno
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Pneumococcal Choline-Binding Proteins Involved in Virulence as Vaccine Candidates
Julio Sempere, Mirella Llamosí, Idoia del Río Menéndez, Beatriz López Ruiz, Mirian Domenech, Fernando González-Camacho
Vaccines.2021; 9(2): 181. CrossRef - Immune Responses to Irradiated Pneumococcal Whole Cell Vaccine
Eunbyeol Ko, Soyoung Jeong, Min Yong Jwa, A Reum Kim, Ye-Eun Ha, Sun Kyung Kim, Sungho Jeong, Ki Bum Ahn, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Vaccines.2021; 9(4): 405. CrossRef - Novel Protein-Based Pneumococcal Vaccines: Assessing the Use of Distinct Protein Fragments Instead of Full-Length Proteins as Vaccine Antigens
Theano Lagousi, Paraskevi Basdeki, John Routsias, Vana Spoulou
Vaccines.2019; 7(1): 9. CrossRef - A Novel, Multiple-Antigen Pneumococcal Vaccine Protects against LethalStreptococcus pneumoniaeChallenge
Win-Yan Chan, Claire Entwisle, Giuseppe Ercoli, Elise Ramos-Sevillano, Ann McIlgorm, Paola Cecchini, Christopher Bailey, Oliver Lam, Gail Whiting, Nicola Green, David Goldblatt, Jun X. Wheeler, Jeremy S. Brown, Liise-anne Pirofski
Infection and Immunity.2019;[Epub] CrossRef - Efficacy of whole-cell pneumococcal vaccine in mice: A systematic review and meta-analysis
Mona Mohammadzadeh, Babak Pourakbari, Shima Mahmoudi, Abbas Keshtkar, Mahdi Habibi-Anbouhi, Setareh Mamishi
Microbial Pathogenesis.2018; 122: 122. CrossRef - Construction and evaluation of a whole-cell pneumococcal vaccine candidate
M. Mohammadzadeh, B. Pourakbari, A. Doosti, S. Mahmoudi, M. Habibi-Anbouhi, S. Mamishi
Journal of Applied Microbiology.2018; 125(6): 1901. CrossRef - Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
Min Yong Jwa, Soyoung Jeong, Eun Byeol Ko, A Reum Kim, Hyun Young Kim, Sun Kyung Kim, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2018; 56(8): 579. CrossRef - spd1672, a novel in vivo-induced gene, affects inflammatory response in a murine model of Streptococcus pneumoniae infection
Lingling Gan, Xuemei Zhang, Xiuyu Xu, Wenchun Xu, Chang Lu, Jin Cui, Hong Wang
Canadian Journal of Microbiology.2018; 64(6): 401. CrossRef - Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
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Melinda M. Pettigrew, Mark R. Alderson, Lauren O. Bakaletz, Stephen J. Barenkamp, Anders P. Hakansson, Kevin M. Mason, Johanna Nokso‐Koivisto, Janak Patel, Stephen I. Pelton, Timothy F. Murphy
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Immunology Letters.2016; 179: 47. CrossRef - SEROTYPE-INDEPENDENT VACCINES AGAINST PNEUMOCOCCAL INFECTION
I. B. Semenova, N. A. Mikhailova
Journal of microbiology, epidemiology and immunobiology.2016; 93(4): 76. CrossRef - Compound 48/80 acts as a potent mucosal adjuvant for vaccination against Streptococcus pneumoniae infection in young mice
Lingbin Zeng, Yusi Liu, Hong Wang, Pu Liao, Zhixin Song, Song Gao, Yingying Wu, Xuemei Zhang, Yibing Yin, Wenchun Xu
Vaccine.2015; 33(8): 1008. CrossRef - Mucosal Immunization with the Live Attenuated Vaccine SPY1 Induces Humoral and Th2-Th17-Regulatory T Cell Cellular Immunity and Protects against Pneumococcal Infection
Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef
- SP0454, A Putative Threonine Dehydratase, Is Required For Pneumococcal Virulence In Mice
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WenJuan Yan , Hong Wang , WenChun Xu , KaiFeng Wu , Run Yao , XiuYu Xu , Jie Dong , YanQing Zhang , Wen Zhong , XueMei Zhang
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J. Microbiol. 2012;50(3):511-517. Published online June 30, 2012
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DOI: https://doi.org/10.1007/s12275-012-2014-8
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830
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Increasing pressure in antibiotic resistance and the requirement for the design of new vaccines are the objectives of clarifying the putative virulence factors in pneumococcal infection. In this study, the putative threonine dehydratase sp0454 was inactivated by erythromycin-resistance cassette replacement in Streptococcus pneumoniae CMCC 31203 strain. The sp0454 mutant was tested for cell growth, adherence, colonization, and virulence in a murine model. The Δsp0454 mutant showed decreased ability for colonization and impaired ability to adhere to A549 cells. However, the SP0454 polypeptide or its antiserum did not affect pneumococcal CMCC 31203 adhesion to A549 cells. The sp0454 deletion mutant was less virulent in a murine intranasal infection model. Real-time RT-PCR analysis revealed significant decrease of the pneumococcal surface antigen A expression in the sp0454 mutant. These results suggest that SP0454 contributes to virulence and colonization, which could be explained in part by modulating the expression of other virulence factors, such as psaA in pneumococcal infection.
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Citations
Citations to this article as recorded by - Pneumococcal live attenuated vaccine: strategies, challenges and future perspectives
Marina Yusoff, Chew Chieng Yeo, Malik Amonov
Exploration of Immunology.2026;[Epub] CrossRef - Dual RNA-Seq of Flavobacterium psychrophilum and Its Outer Membrane Vesicles Distinguishes Genes Associated with Susceptibility to Bacterial Cold-Water Disease in Rainbow Trout (Oncorhynchus mykiss)
Pratima Chapagain, Ali Ali, Mohamed Salem
Pathogens.2023; 12(3): 436. CrossRef - Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Journal of Microbiology.2017; 55(10): 823. CrossRef - Mucosal Immunization with the Live Attenuated Vaccine SPY1 Induces Humoral and Th2-Th17-Regulatory T Cell Cellular Immunity and Protects against Pneumococcal Infection
Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef - A Novel Protein, RafX, Is Important for Common Cell Wall Polysaccharide Biosynthesis in Streptococcus pneumoniae: Implications for Bacterial Virulence
Kaifeng Wu, Jian Huang, Yanqing Zhang, Wenchun Xu, Hongmei Xu, Libin Wang, Ju Cao, Xuemei Zhang, Yibing Yin
Journal of Bacteriology.2014; 196(18): 3324. CrossRef - Serotype-independent protection against pneumococcal infections elicited by intranasal immunization with ethanol-killed pneumococcal strain, SPY1
Xiuyu Xu, Jiangping Meng, Yiping Wang, Jie Zheng, Kaifeng Wu, Xuemei Zhang, Yibing Yin, Qun Zhang
Journal of Microbiology.2014; 52(4): 315. CrossRef
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