Journal of Microbiology

Kai Dou

2 Articles

Trichoderma biodiversity in major ecological systems of China: Kai Dou , Jinxin Gao , Chulong Zhang , Hetong Yang , Xiliang Jiang , Jishun Li , Yaqian Li , Wei Wang , Hongquan Xian , Shigui Li , Yan Liu , Jindong Hu , Jie Chen; J. Microbiol. 2019;57(8):668-675. Published online May 23, 2019; DOI: https://doi.org/10.1007/s12275-019-8357-7

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An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.

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Functional Analysis of a Subtilisin-like Serine Protease Gene from Biocontrol Fungus Trichoderma harzianum: Haijuan Fan , Zhihua Liu , Rongshu Zhang , Na Wang , Kai Dou , Gulijimila Mijiti , Guiping Diao , Zhiying Wang; J. Microbiol. 2014;52(2):129-138. Published online February 1, 2014; DOI: https://doi.org/10.1007/s12275-014-3308-9

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Abstract PDF

The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371. Its coding region is 1302 bp in length, encoding 433 amino acids, with a predicted protein molecular weight of 44.9 kDa and pI of 5.91. ThSS45 was shown by RT-qPCR analysis to be differentially transcribed in response to eight different treatments. The transcription of ThSS45 was up-regulated when grown in mineral medium, under carbon starvation, and nitrogen starvation, and in the presence of 1% root powder, 1% stem powder, and 1% leaf powder derived from Populus davidiana × P. bolleana (Shanxin poplar) aseptic seedlings. The highest increase in transcription approached 3.5-fold that of the control at 6 h under induction with 1% poplar root powder. The transcription of ThSS45 was also slightly up-regulated by 1% Alternaria alternata cell wall and 5% A. alternata fermentation liquid. Moreover, the analyses of coding and promoter regions of ThSS45 homologs indicated that serine protease may be involved in both mycoparasitism and antibiotic secretion. ThSS45 was cloned into the pGEX-4T-2 vector and then expressed in Escherichia coli BL21. The recombinant protein, with an expected molecular weight of approximately 69 kDa, was then purified. When transformant BL21-ss was induced with 1 mM IPTG for 6 h, the purified protease activity reached a peak of 18.25 U/ml at pH 7.0 and 40°C. In antifungal assays the purified protease obviously inhibited the growth of A. alternata mycelia.

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Genome Biology and Evolution.2021;[Epub] CrossRef
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AMB Express.2020;[Epub] CrossRef
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Raffael C. Inglin, Alessia I. Delbrück, Benjamin Fässler, Katharina E. Siebenmann, Christophe Lacroix, Marc J. A. Stevens, Leo Meile
Journal of Food Safety.2018;[Epub] CrossRef
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Enzyme and Microbial Technology.2018; 112: 35. CrossRef
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Wenjing Yu, Gulijimila Mijiti, Ying Huang, Haijuan Fan, Yucheng Wang, Zhihua Liu
Scientific Reports.2018;[Epub] CrossRef
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BMC Evolutionary Biology.2018;[Epub] CrossRef
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Journal of Plant Biochemistry and Biotechnology.2018; 27(3): 351. CrossRef
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Biocontrol Science.2017; 22(3): 145. CrossRef
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International Journal of Biological Macromolecules.2016; 91: 961. CrossRef
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Current Microbiology.2016; 73(3): 419. CrossRef
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