- Metabolomic profiling reveals enrichment of cordycepin in senescence process of Cordyceps militaris fruit bodies
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Junsang Oh , Deok-Hyo Yoon , Bhushan Shrestha , Hyung-Kyoon Choi , Gi-Ho Sung
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J. Microbiol. 2019;57(1):54-63. Published online December 29, 2018
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DOI: https://doi.org/10.1007/s12275-019-8486-z
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 Abstract
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Cordyceps militaris is a species of Cordyceps that is classified
in the Cordycipitaceae family and is well known in East Asia
as a traditional medicinal mushroom. Its artificial fruit body
has been widely cultivated for commercial use in cosmetics,
functional food, and medicine. To explore the metabolites
associated with fruit body development, we conducted gas
chromatography mass spectrometry (GC-MS) analyses based
on developmental stage, which was divided into the growth
period (stage 1, stage 2, and stage 3) and aging period (stage
4). We detected 39 biochemical metabolites associated with
nucleotide, carbohydrate, and amino acid metabolism. Cordycepin,
one of the representative bioactive compounds in
C. militaris, was significantly enriched in stage 4 of aging period
and is associated with glucose accumulation. The accumulation
of cordycepin in stage 4 of aging period also seems
to be related to the glutamine and glutamic acid pathway. Our
results
also showed enrichment of other bioactive compounds
such as mannitol and xylitol in stage 4 of aging period. Our
metabolomic profiling based on the developmental stages of
C. militaris is useful for exploring bioactive compounds (e.g.,
cordycepin, mannitol, GABA, and xylitol) that are enriched
in stage 4 of aging period and understanding the biosynthetic
mechanisms associated with cordycepin production. Through
optimization of fruit body cultivation by selecting stage 4 of
aging period as a harvesting time, our findings can be utilized
in food and medical applications of C. militaris in future.
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Citations
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Foods.2025; 14(19): 3408. CrossRef - Light-Exposed Metabolic Responses of Cordyceps militaris through Transcriptome-Integrated Genome-Scale Modeling
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Effects on total phenolic and flavonoid content, antioxidant properties, and angiotensin I-converting enzyme inhibitory activity of beans by solid-state fermentation with
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- Species identity of Phellinus linteus (sanghuang) extensively used as a medicinal mushroom in Korea
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Jae-Gu Han , Min-Woo Hyun , Chang Sun Kim , Jong Won Jo , Jae-Han Cho , Kang-Hyo Lee , Won-Sik Kong , Sang-Kuk Han , Junsang Oh , Gi-Ho Sung
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J. Microbiol. 2016;54(4):290-295. Published online April 1, 2016
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DOI: https://doi.org/10.1007/s12275-016-5520-2
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1,376
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2
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29
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Abstract
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Sanghuang is a medicinal mushroom that has gained particular
attention in Korea. It has been extensively studied for
the past few decades as a natural immune booster and cancer
suppressor. Although the scientific name, Phellinus linteus,
has been commonly used to refer to the sanghuang mushroom,
the species identity of sanghuang has been called into question
due to the ambiguity of its circumscription and the inadequacy
of morphological distinctions within allied species. Because
the species concept of sanghuang has been elucidated by recent
molecular phylogenetic studies, it has become necessary
to clarify the taxonomic positions of sanghuang strains extensively
utilized in Korea. We conducted a phylogenetic analysis
of 74 strains belonging to the P. linteus-baumii complex based
on ITS nrDNA sequences. Parental stains of sanghuang varieties
formally registered in the Korea Seed & Variety Service,
including ASI 26046 (Corea sanghuang), 26114 (Boolro), and
26115 (HK 1-ho) were grouped with Sanghuangporus sanghuang
instead of P. linteus in the inferred phylogeny.
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