- Influences of genetically perturbing synthesis of the typical yellow pigment on conidiation, cell wall integrity, stress tolerance, and cellulase production in Trichoderma reesei
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Weixin Zhang , Ning An , Junqi Guo , Zhixing Wang , Xiangfeng Meng , Weifeng Liu
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J. Microbiol. 2021;59(4):426-434. Published online January 26, 2021
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DOI: https://doi.org/10.1007/s12275-021-0433-0
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The prominent protein producing workhorse Trichoderma
reesei secretes a typical yellow pigment that is synthesized
by a gene cluster including two polyketide synthase encoding
genes sor1 and sor2. Two transcription factors (YPR1 and
YPR2) that are encoded in the same cluster have been shown
to regulate the expression of the sor genes. However, the physiological
relevance of the yellow pigment synthesis in T.
reesei is not completely clear. In this study, a yellow pigment
hyper-producer OEypr1 and three yellow pigment non-producers,
OEypr1-sor1, Δypr1, and OEypr2, were constructed.
Their phenotypic features in mycelial growth, conidiation,
cell wall integrity, stress tolerance, and cellulase production
were determined. Whereas hyperproduction of the yellow pigment
caused significant defects in all the physiological aspects
tested, the non-producers showed similar colony growth, but
improved conidiation, maintenance of cell wall integrity, and
stress tolerance compared to the control strain. Moreover, in
contrast to the severely compromised extracellular cellobiohydrolase
production in the yellow pigment hyperproducer,
loss of the yellow pigment hardly affected induced cellulase
gene expression. Our results demonstrate that interfering with
the yellow pigment synthesis constitutes an engineering strategy
to endow T. reesei with preferred features for industrial
application.
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Citations
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