- Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory
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Lingmin Jiang , Dexin Wang , Jung-Sook Lee , Dae-Hyuk Kim , Jae Cheol Jeong , Cha Young Kim , Suk Weon Kim , Jiyoung Lee
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J. Microbiol. 2020;58(5):357-366. Published online March 27, 2020
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DOI: https://doi.org/10.1007/s12275-020-9294-1
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Correction in: J. Microbiol 2025;63(8):e2508100
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Strain KSNA2T, a Gram-negative, moderately halophilic, facultatively
anaerobic, motile, rod-shaped bacterium, was isolated
from the surface-sterilized stem tissue of a beach morning
glory (Calystegia soldanella) plant in Chuja Island, Jejudo,
Republic of Korea. Phylogenetic analysis based on 16S
rRNA gene and whole-genome sequences revealed that strain
KSNA2T formed a distinct lineage within the family Enterobacteriaceae,
with the highest 16S rRNA gene sequence similarity
to Izhakiella australiensis KCTC 72143T (96.2%) and
Izhakiella capsodis KCTC 72142T (96.0%), exhibited 95.5–
95.9% similarity to other genera in the family Enterobacteriaceae
and Erwiniaceae. Conserved signature indels analysis
elucidated that strain KSNA2T was delimited into family
Enterobacteriaceae. KSNA2T genome comprises a circular
chromosome of 5,182,800 bp with 56.1% G + C content. Digital
DNA-DNA relatedness levels between strain KSNA2T
and 18 closely related species were 19.3 to 21.1%. Average
nucleotide identity values were between 72.0 and 76.7%.
Growth of strain KSNA2T was observed at 4 to 45°C (optimum,
25°C) and pH 5.0 to 12.0 (optimum, pH 7.0) in the
presence of 0 to 11% (w/v) NaCl (optimum, 0–7%). The major
cellular fatty acids (> 10%) were C16:0 followed by summed
feature 8 (C18:1 ω7c and/or C18:1 ω6c), summed feature
3 (C16:1 ω7c and/or C16:1 ω6c), C17:0 cyclo, and C14:0. The major
isoprenoid quinone was ubiquinone-8 (Q-8). With combined
phylogenetic, genomic, phenotypic, and chemotaxonomic
features, strain KSNA2T represents a novel species of
a new genus in the family Enterobacteriaceae, for which the
name Jejubacter calystegiae gen. nov., sp. nov. is proposed.
The type strain is KSNA2T (= KCTC 72234T = CCTCC AB
2019098T).
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Citations
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- Pseudoxanthomonas sp. JBR18, a halotolerant endophytic bacterium, improves the salt tolerance of Arabidopsis seedlings
Yuxin Peng, Lingmin Jiang, Doeun Jeon, Donghyun Cho, Youngmin Kim, Cha Young Kim, Ju Huck Lee, Jiyoung Lee Plant Physiology and Biochemistry.2024; 207: 108415. CrossRef - Two microbes assisting Miscanthus floridulus in remediating multi-metal(loid)s-contaminated soil
Yunhua Xiao, Jingjing Ma, Rui Chen, Sha Xiang, Bo Yang, Liang Chen, Jun Fang, Shuming Liu Environmental Science and Pollution Research.2024; 31(20): 28922. CrossRef - Improving plant salt tolerance through Algoriphagus halophytocola sp. nov., isolated from the halophyte Salicornia europaea
Yuxin Peng, Dong Hyun Cho, Zalfa Humaira, Yu Lim Park, Ki Hyun Kim, Cha Young Kim, Jiyoung Lee Frontiers in Microbiology.2024;[Epub] CrossRef - Detection of human pathogenic bacteria in rectal DNA samples from Zalophus californianus in the Gulf of California, Mexico
Francesco Cicala, David Ramírez-Delgado, Ricardo Gómez-Reyes, Marcel Martínez-Porchas, Jorge Rojas-Vargas, Liliana Pardo-López, Alexei F. Licea-Navarro Scientific Reports.2022;[Epub] CrossRef - Genome Insights into the Novel Species Jejubacter calystegiae, a Plant Growth-Promoting Bacterium in Saline Conditions
Ling Min Jiang, Yong Jae Lee, Ho Le Han, Myoung Hui Lee, Jae Cheol Jeong, Cha Young Kim, Suk Weon Kim, Ji Young Lee Diversity.2021; 13(1): 24. CrossRef -
Pedobacter endophyticus sp. nov., an endophytic bacterium isolated from Carex pumila
Yuxin Peng, Lingmin Jiang, Jiyoon Seo, Zhun Li, Hanna Choe, Jae Cheol Jeong, Suk Weon Kim, Young-Min Kim, Cha Young Kim, Jiyoung Lee
International Journal of Systematic and Evolutionary Microbiology
.2021;[Epub] CrossRef -
The Changing Face of the Family
Enterobacteriaceae
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Enterobacterales
”): New Members, Taxonomic Issues, Geographic Expansion, and New Diseases and Disease Syndromes
J. Michael Janda, Sharon L. Abbott Clinical Microbiology Reviews.2021;[Epub] CrossRef
- Sutterella faecalis sp. nov., isolated from human faeces
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Byeong Seob Oh , Ji-Sun Kim , Seung Yeob Yu , Seoung Woo Ryu , Seung-Hwan Park , Se Won Kang , Jam-Eon Park , Seung-Hyeon Choi , Kook-Il Han , Keun Chul Lee , Mi Kyung Eom , Min Kuk Suh , Han Sol Kim , Dong Ho Lee , Hyuk Yoon , Byung-Yong Kim , Je Hee Lee , Jung-Sook Lee , Ju Huck Lee
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J. Microbiol. 2020;58(2):99-104. Published online January 29, 2020
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DOI: https://doi.org/10.1007/s12275-020-9396-9
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An obligately anaerobic, Gram-stain-negative, non-motile,
non-spore-forming, and coccobacilli-shaped bacterial strain,
designated KGMB03119T, was isolated from human faeces
from a Korean. Phylogenetic analysis based on the 16S rRNA
gene sequence revealed that the isolate was a member of the
genus Sutterella and most closely related to Sutterlla wadsworthensis
KCTC 15691T (96.8% 16S rRNA gene sequence
similarity). The DNA G + C content of strain KGMB03119T
was 58.3 mol% as determined from its whole genome sequence.
Strain KGMB03119T was asaccharolytic, catalase-positive,
oxidase- and urease-negative. Furthermore, the isolate
was positive for alkaline phosphatase, leucine arylamidase,
acid phosphatase, arginine arylamidase, alanine arylamidase,
and glycine arylamidase. The major cellular fatty acids (> 10%)
of the isolate were C18:1ω9c and C16:0. Methylmenaquinone-5
(MMK-5, 100%) was the predominant isoprenoid quinone
in the isolate. Based on the phylogenetic, physiological, and
chemotaxonomic characteristics, strain KGMB03119T represents
a novel species, for which the name Sutterella faecalis
sp. nov. is proposed. The type strain is KGMB03119T (= KCTC
15823T = NBRC 114254T).
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Lauryn Langford, Dhara D. Shah International Journal of Molecular Sciences.2024; 25(5): 2983. CrossRef - Validation List no. 212. Valid publication of new names and new combinations effectively published outside the IJSEM
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- Anaerotignum faecicola sp. nov., isolated from human faeces
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Seung-Hyeon Choi , Ji-Sun Kim , Jam-Eon Park , Keun Chul Lee , Mi Kyung Eom , Byeong Seob Oh , Seung Yeob Yu , Se Won Kang , Kook-Il Han , Min Kuk Suh , Dong Ho Lee , Hyuk Yoon , Byung-Yong Kim , Je Hee Lee , Ju Huck Lee , Jung-Sook Lee , Seung-Hwan Park
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J. Microbiol. 2019;57(12):1073-1078. Published online November 4, 2019
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DOI: https://doi.org/10.1007/s12275-019-9268-3
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527
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11
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A strictly anaerobic bacterium, designated as strain KGMB-
03357T, was isolated from the faeces of a healthy Korean selected
by Bundang Seoul National University based on health
status. Cells of strain KGMB03357T are Gram-stain-positive,
non-motile, non-spore-forming, and observed as straight or
curved rods. The isolate grew at 10–45°C (optimum temperature
of 40°C) and a pH range of 5.1–10.5 (optimum pH of
6.8). Analysis of phylogenetic trees based on the 16S rRNA
gene sequences revealed that strain KGMB03357T forms a
lineage within the genus Anaerotignum, and is most closely
related to Anaerotignum lactatifermentans G17T (= KCTC
15066T, 96.1%), Anaerotignum propionicum DSM 1682T (=
KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM
03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans
SH021T (= KCTC 15705T, 94.8%). The ANI values
between strain KGMB 03357T and members of the genus
Anaerotignum were 73.3–71.0%, which are below the ANI
criterion for interspecies identity. The DNA G + C content
based on the whole-genome sequence is 47.3 mol%. The major
cellular fatty acids of strain KGMB03357T are C16:0, C18:0,
C18:1 cis 9, and anteiso-C15:0. Strain KGMB03357T contains
meso-diaminopimelic acid as the diagnostic amino acid in
the cell wall peptidoglycan. Based on the phenotypic, phylogenetic,
and genomic properties, strain KGMB 03357T represents
a novel species of the genus Anaerotignum, for which
the name Anaerotignum faecicola sp. nov. is proposed. The
type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).
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Ji-Sun Kim , Keun Chul Lee , Min Kuk Suh , Kook-Il Han , Mi Kyung Eom , Ju Huck Lee , Seung-Hwan Park , Se Won Kang , Jam-Eon Park , Byeong Seob Oh , Seung Yeob Yu , Seung-Hyeon Choi , Dong Ho Lee , Hyuk Yoon , Byung-Yong Kim , Seung-Jo Yang , Jung-Sook Lee
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J. Microbiol. 2019;57(1):38-44. Published online December 29, 2018
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DOI: https://doi.org/10.1007/s12275-019-8550-8
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458
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A Gram-stain-positive, obligately anaerobic, non-motile, nonspore-
forming, and rod-shaped bacterial strain, designated
KGMB01110T, was isolated from a faecal sample of a healthy
male in South Korea. Phylogenetic analysis based on 16S rRNA
gene showed that strain KGMB01110T belonged to Clostridium
cluster XIVa and was most closely related to Mediterraneibacter
glycyrrhizinilyticus KCTC 5760T (95.9% 16S rRNA
gene sequence similarity). The DNA G + C content of strain
KGMB01110T based on its whole genome sequence was 44.1
mol%. The major cellular fatty acids (> 10%) of the isolate
were C14:0 and C16:0. The strain KGMB01110T was positive for
arginine dihydrolase, β-galactosidase-6-phosphatase, and
alkaline phosphatase. The strain KGMB01110T also produced
acid from D-glucose and D-rhamnose, and hydrolyzed gelatin
and aesculin. Furthermore, HPLC analysis and UV-tests
of culture supernatant revealed that the strain KGMB01110T
produced butyrate as the major end product of glucose fermentation.
Based on the phylogenetic and phenotypic characteristics,
strain KGMB01110T represent a novel species of
the genus Mediterraneibacter in the family Lachnospiraceae.
The type strain is KGMB01110T (= KCTC 15684T = CCUG
72830T).
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