- Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation
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Hae-In Jung , Yun-Jung Kim , Yun-Jung Lee , Hee-Soo Lee , Jung-Kee Lee , Soo-Ki Kim
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J. Microbiol. 2017;55(10):800-808. Published online September 28, 2017
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DOI: https://doi.org/10.1007/s12275-017-7374-7
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Burkholderia sp. is a gram-negative bacterium that commonly
exists in the environment, and can cause diseases in plants,
animals, and humans. Here, a transposon mutant library of
a Burkholderia lata isolate from a pig with swine respiratory
disease in Korea was screened for strains showing attenuated
virulence in Caenorhabditis elegans. One such mutant was
obtained, and the Tn5 insertion junction was mapped to
rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that
functions as a receptor. Mutation of rpfR caused a reduction
in growth on CPG agar and swimming motility as well as a
rough colony morphology on Congo red agar. TLC analysis
showed reduced AHL secretion, which was in agreement with
the results from plate-based and bioluminescence assays. The
mutant strain produced significantly more biofilm detected
by crystal violet staining than the parent strain. SEM of the
mutant strain clearly showed that the overproduced biofilm
contained a filamentous structure. These results suggest
that the cyclic di-GMP phosphodiesterase RpfR plays an important
role in quorum sensing modulation of the bacterial
virulence and biofilm formation.
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Citations
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