Journal of Microbiology

Jung-Ae Kim

2 Articles

Characterization of a Mutant Strain of a Filamentous Fungus Cladosporium phlei for the Mass Production of the Secondary Metabolite Phleichrome: Min-Hee Yi , Jung-Ae Kim , Jung-Mi Kim , Jin-Ah Park , Beom-Tae Kim , Seung-Moon Park , Moon-Sik Yang , Ki-Jun Hwang , Dae-Hyuk Kim; J. Microbiol. 2011;49(4):680-683. Published online September 2, 2011; DOI: https://doi.org/10.1007/s12275-011-1022-4

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UV-mutagenesis was performed to obtain mutant strains that demonstrate altered production of phleichrome, a secondary metabolite of Cladosporium phlei. Among fifty mutants selected, based on the increased area and intensity of the purple pigment surrounding the colonies, the strain M0035 showed the highest production of phleichrome, more than seven fold over wild type. Plate cultures of the M0035 strain resulted in a total of 592 mg phleichrome consisting of 146 mg and 446 mg from the mycelia and agar media, respectively. The M0035 strain displayed a growth rate and a mycelial mass comparable to the parental strain but had significantly reduced asexual sporulation.

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One-step synthesis of novel phaeosphaerin derivatives for photocytotoxicity
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Dyes and Pigments.2025; 235: 112613. CrossRef
Metabolic engineering of Saccharomyces cerevisiae for the biosynthesis of a fungal pigment from the phytopathogenic fungus Cladosporium phlei
Yeji Gwon, Kum-Kang So, Jeesun Chun, Dae-Hyuk Kim
Journal of Biological Engineering.2024;[Epub] CrossRef
Improvement of the Biosynthesis of Resveratrol in Endophytic Fungus (Alternaria sp. MG1) by the Synergistic Effect of UV Light and Oligomeric Proanthocyanidins
Yao Lu, Junling Shi, Xixi Zhao, Yuyang Song, Yi Qin, Yanlin Liu
Frontiers in Microbiology.2021;[Epub] CrossRef
Draft Genome Sequencing of the Pathogenic Fungus Cladosporium phlei ATCC 36193 Identifies Candidates of Novel Polyketide Synthase Genes Involved in Perylenequinone-Group Pigment Production
Yu-Na Kang, Kum-Kang So, Do-Wan Kim, Dae-Hyuk Kim, Tae-Ho Lee
Evolutionary Bioinformatics.2019;[Epub] CrossRef
Identification of a Polyketide Synthase Gene in the Synthesis of Phleichrome of the Phytopathogenic Fungus Cladosporium phlei
Kum-Kang So, Yun-Jo Chung, Jung-Mi Kim, Beom-Tae Kim, Seung-Moon Park, Dae-Hyuk Kim
Molecules and Cells.2015; 38(12): 1105. CrossRef
Violaceomyces palustris gen. et sp. nov. and a new monotypic lineage, Violaceomycetales ord. nov. in Ustilaginomycetes
Sebastian Albu, Merje Toome, M. Catherine Aime
Mycologia.2015; 107(6): 1193. CrossRef
Efficient strategy for maintaining and enhancing the huperzine A production of Shiraia sp. Slf14 through inducer elicitation
Riming Yan, Zhibin Zhang, Ya Wang, Huilin Yang, Qinggui Zeng, Du Zhu
Journal of Industrial Microbiology and Biotechnology.2014; 41(7): 1175. CrossRef
Rapid screening of an ordered fosmid library to clone multiple polyketide synthase genes of the phytopathogenic fungus Cladosporium phlei
Kum-Kang So, Jung-Mi Kim, Ngoc-Luong Nguyen, Jin-Ah Park, Beom-Tae Kim, Seung-Moon Park, Ki-Jun Hwang, Dae-Hyuk Kim
Journal of Microbiological Methods.2012; 91(3): 412. CrossRef

Molecular Diversity of Chrysoviruses in Korean Isolates of A New Fungal Species, Cryphonectria nitschkei: Jung-Mi Kim , Jung-Ae Kim , Jin-Ah Park , Seung-Moon Park , Byeong-Jin Cha , Moon-Sik Yang , Dae-Hyuk Kim; J. Microbiol. 2009;47(4):441-447. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0206-7

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Abstract PDF

Genetic diversity of the chrysovirus within the four fungal strains was analyzed by comparing the full- length sequences of cloned chrysoviral genes encoding the RNA-dependent RNA polymerase (RdRp) and capsid protein (CP). Because the morphological characteristics of four chrysovirus-infected Cryphonectria spp. strains were different, strain identification was conducted via sequence comparison of the internal transcribed spacers (ITSs) of the fungal rRNA gene. Phylogenic analysis of the ITS regions revealed that the four strains were closely clustered with the reference strain of Cryphonectria nitschkei, while they were more distantly related to other common Cryphonectria species, indicating that they were likely C. nitschkei. Sequence comparison among chrysoviruses from Korean C. nitschkei strains revealed that similarities of the RdRp and CP genes ranged from 98% to 100% and from 95% to 100%, respectively, at the protein level. Their corresponding nucleotide sequences showed 97% to 100% and 84% to 100% identities, respectively. Compared to RdRp, the CP gene had more divergence, suggesting the presence of genes possessing different evolutionary rates within the chrysovirus genome. Sequence comparisons with other known chrysoviruses showed that the four Korean chrysoviruses were clustered together at the next lineage level. Discovering why two strains (bs131 and bs132) containing identical ITS sequences and chrysoviruses display different phenotypes should prove interesting.

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Incidence of diverse dsRNA mycoviruses inTrichodermaspp. causing green mold disease of shiitakeLentinula edodes
Suk-Hyun Yun, Song Hee Lee, Kum-Kang So, Jung-Mi Kim, Dae-Hyuk Kim, Rich Boden
FEMS Microbiology Letters.2016; 363(19): fnw220. CrossRef
Molecular characterization of a novel mycovirus in the cultivated mushroom, Lentinula edodes
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Virology Journal.2012;[Epub] CrossRef
Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb.
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Virus Research.2011; 159(1): 73. CrossRef
Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus
Atif Jamal, Elaine M. Bignell, Robert H.A. Coutts
Virus Research.2010; 153(1): 64. CrossRef
Nucleotide sequences of four segments of chrysovirus in Korean Cryphonectria nitschkei BS122 strain
Jung-Mi Kim, Jin-Ah Park, Seung-Moon Park, Byeong-Jin Cha, Moon-Sik Yang, Dae-Hyuk Kim
Virus Genes.2010; 41(2): 292. CrossRef

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