Journal of Microbiology

Jung Hun Lee

3 Articles

Nomenclature of ISCR1 Elements Capable of Mobilizing Antibiotic Resistance Genes Present in Complex Class 1 Integrons: Seung Ghyu Sohn , Jae Jin Lee , Jae Seok Song , Jung Hun Lee , Ha Ik Sun , Kwang Seung Park , Il Kwon Bae , Jung-Hyun Lee , Byeong Chul Jeong , Sang Hee Lee; J. Microbiol. 2009;47(4):514-516. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0054-5

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The dissemination of many antibiotic resistance genes has arisen among members of the family Enterobacteriaceae. The dissemination mechanism of these antibiotic resistance genes is closely linked with insertion sequence common region 1 (ISCR1). Thus, caution must be taken in clinical settings to prevent further dissemination of these antibiotic resistance genes. A nomenclature system of ISCR1 variants, important for the antibiotic resistance dissemination, was proposed. The proposed system can designate all ISCR1 variants on the basis of the detection time and by considering amino-acid substitution(s) compared with ISCR1a. This nomenclature system of ISCR1 variants can be applied to 19 groups (ISCR1 to ISCR19) of the ISCR family and help some researchers to correctly designate new ISCR subgroups.

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Molecular Characteristics of Carbapenem-Resistant Gram-Negative Bacteria in Southern China
Fen Zheng, Jingjing Sun, Cancan Cheng, Yongyu Rui
Microbial Drug Resistance.2015; 21(2): 178. CrossRef
Novel ISCR1-linked resistance genes found in multidrug-resistant Gram-negative bacteria in southern China
Fengping Wang, Kuihai Wu, Jingjing Sun, Qian Wang, Qing Chen, Shouyi Yu, Yongyu Rui
International Journal of Antimicrobial Agents.2012; 40(5): 404. CrossRef
Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens
Hatch W. Stokes, Michael R. Gillings
FEMS Microbiology Reviews.2011; 35(5): 790. CrossRef

Removal of Contaminating TEM-la β-Lactamase Gene from Removal of Contaminating TEM-la β-Lactamase Gene from: Jae Seok Song , Jung Hun Lee , Jung-Hyun Lee , Byeong Chul Jeong , Won-Keun Lee , Sang Hee Lee; J. Microbiol. 2006;44(1):126-128.; DOI: https://doi.org/2326 [pii]

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Abstract PDF: This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1a gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

Molecular Characterization of TEM-type [beta]-Lactamases Identified in Cold-Seep Sediments of Edison Seamount (South of Lihir Island, Papua New Guinea): Jae Seok Song , Jeong Ho Jeon , Jung Hun Lee , Seok Hoon Jeong , Byeong Chul Jeong , Sang-Jin Kim , Jung-Hyun Lee , Sang Hee Lee; J. Microbiol. 2005;43(2):172-178.; DOI: https://doi.org/2165 [pii]

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Abstract PDF: To determine the prevalence and genotypes of [beta]-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced [beta]-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum [beta]-lactamase. 837- and 259-bp fragments specific to bla_TEM genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 was the most prevalent [beta]-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type [beta]-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type [beta]-lactamases of the pre-antibiotic era indicates that TEM-type [beta]-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

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