- Isolation and Characterization of a Reducing Polyketide Synthase Gene from the Lichen-Forming Fungus Usnea longissima
-
Yi Wang , Jung A Kim , Yong Hwa Cheong , Yogesh Joshi , Young Jin Koh , Jae-Seoun Hur
-
J. Microbiol. 2011;49(3):473-480. Published online June 30, 2011
-
DOI: https://doi.org/10.1007/s12275-011-0362-4
-
-
740
View
-
0
Download
-
9
Crossref
-
 Abstract
 PDF
-
The reducing polyketide synthases found in filamentous fungi are involved in the biosynthesis of many drugs and toxins. Lichens produce bioactive polyketides, but the roles of reducing polyketide synthases in lichens remain to be clearly elucidated. In this study, a reducing polyketide synthase gene (UlPKS3) was isolated and characterized from a cultured mycobiont of Usnea longissima. Complete sequence information regarding UlPKS3 (6,519 bp) was obtained by screening a fosmid genomic library. A UlPKS3 sequence analysis suggested that it contains features of a reducing fungal type I polyketide synthase with β-ketoacyl synthase (KS), acyltransferase (AT), dehydratase (DH), enoyl reductase (ER), ketoacyl reducatse (KR), and acyl carrier protein (ACP) domains. This domain structure was similar to the structure of ccRads1, which is known to be involved in resorcylic acid lactone biosynthesis in Chaetomium chiversii. The results of phylogenetic analysis located UlPKS3 in the clade of reducing polyketide synthases. RT-PCR analysis results demonstrated that UlPKS3 had six intervening introns and that UlPKS3 expression was upregulated by glucose, sorbitol, inositol, and mannitol.
-
Citations
Citations to this article as recorded by  - Genomic Features of Taiwanofungus gaoligongensis and the Transcriptional Regulation of Secondary Metabolite Biosynthesis
Yadong Zhang, Yi Wang, Xiaolong Yuan, Hongling Zhang, Yuan Zheng
Journal of Fungi.2024; 10(12): 826. CrossRef - Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation
Jianhua Guo, Xiaoxu Zhao, Jie Shi
Biotechnology Progress.2024;[Epub] CrossRef - Discovery and excavation of lichen bioactive natural products
Meirong Ren, Shuhua Jiang, Yanyan Wang, Xinhua Pan, Feng Pan, Xinli Wei
Frontiers in Microbiology.2023;[Epub] CrossRef - Lichen Biosynthetic Gene Clusters. Part I. Genome Sequencing Reveals a Rich Biosynthetic Potential
Robert L. Bertrand, Mona Abdel-Hameed, John L. Sorensen
Journal of Natural Products.2018; 81(4): 723. CrossRef - Tissue-specific localization of polyketide synthase and other associated genes in the lichen, Cladonia rangiferina, using laser microdissection
Mostafa E. Elshobary, Michael G. Becker, Jenna L. Kalichuk, Ainsley C. Chan, Mark F. Belmonte, Michele D. Piercey-Normore
Phytochemistry.2018; 156: 142. CrossRef - A comprehensive catalogue of polyketide synthase gene clusters in lichenizing fungi
Robert L Bertrand, John L Sorensen
Journal of Industrial Microbiology and Biotechnology.2018; 45(12): 1067. CrossRef - In vitro risk assessment of usnic acid
Zühal Polat, Elanur Aydın, Hasan Türkez, Ali Aslan
Toxicology and Industrial Health.2016; 32(3): 468. CrossRef - Algal carbohydrates affect polyketide synthesis of the lichen-forming fungusCladonia rangiferina
Mostafa E. Elshobary, Mohamed E. Osman, Atef M. Abo-Shady, Emy Komatsu, Hélène Perreault, John Sorensen, Michele D. Piercey-Normore
Mycologia.2016; 108(4): 646. CrossRef - Genome characteristics reveal the impact of lichenization on lichen-forming fungus Endocarpon pusillum Hedwig (Verrucariales, Ascomycota)
Yan-Yan Wang, Bin Liu, Xin-Yu Zhang, Qi-Ming Zhou, Tao Zhang, Hui Li, Yu-Fei Yu, Xiao-Ling Zhang, Xi-Yan Hao, Meng Wang, Lei Wang, Jiang-Chun Wei
BMC Genomics.2014; 15(1): 34. CrossRef
|
MSK
