- Cyclic Dipeptides from Lactic Acid Bacteria Inhibit Proliferation of the Influenza A Virus
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Min-Kyu Kwak , Rui Liu , Jun-Oh Kwon , Min-Kyu Kim , Andrew HyoungJin Kim , Sa-Ouk Kang
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J. Microbiol. 2013;51(6):836-843. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3521-y
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Abstract
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We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp.,
and Lactococcus lactis.
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