- Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model
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Ming Zhang , Xing Fan , Bing Fang , Chengzhen Zhu , Jun Zhu , Fazheng Ren
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J. Microbiol. 2015;53(6):398-405. Published online May 30, 2015
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DOI: https://doi.org/10.1007/s12275-015-5046-z
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Probiotics have been suggested as a prophylactic measure
in colon cancer. The aim of this study was to investigate the
impact of Lactobacillus salivarius Ren (Ren) in modulating
colonic microbiota structure and colon cancer incidence in a
rat model after injection with 1,2-dimethyl hydrazine (DMH).
The results indicated that oral administration of Ren could
effectively suppress DMH-induced colonic carcinogenesis.
A significant decrease in cancer incidence (87.5% to 25%)
was detected in rats fed with a dose of 5 × 1010 CFU/kg
bodyweight per day. Using denaturing gradient gel electrophoresis
and Real-time PCR combined with multivariate
statistical methods, we demonstrated that injection with DMH
significantly altered the rat gut microbiota, while Ren counteracted
these DMH-induced adverse effects and promoted
reversion of the gut microbiota close to the healthy state. Tvalue
biplots followed by band sequencing identified 21 bacterial
strains as critical variables affected by DMH and Ren.
Injection of DMH significantly increased the amount of
Ruminococcus species (sp.) and Clostridiales bacteria, as well
as decreasing the Prevotella sp. Administration of Ren reduced
the amount of Ruminococcus sp., Clostridiales bacteria, and
Bacteroides dorei, and increased the amount of Prevotella.
Real-time PCR results were consistent with the results derived
by t-value biplots. These findings suggested that Ren is a potential
agent for colon cancer prevention. In conclusion, the
results
in the present study suggest a potential therapeutic
approach based on the modulation of intestinal microflora
by probiotics may be beneficial in the prevention of colorectal
carcinogenesis.
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Citations
Citations to this article as recorded by 
- Morphological Structure of Propagules and Electrophoretic Karyotype Analysis of False Smut Villosiclava virens in Rice
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Rongtao Fu , Lei Ding , Jun Zhu , Ping Li , Ai-ping Zheng
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J. Microbiol. 2012;50(2):263-269. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1456-3
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680
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1
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29
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Abstract
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The target pathogen Villosiclava virens (teleomorph: claviceps
oryzae-sativae) was isolated from the infected rice, where it
caused false smut. In our study, the forming processes of the
chlamydospores, chlamydospore balls, conidiospores, and
secondary conidiospores during the asexual reproduction
were observed more precisely and in greater detail than
previous descriptions. The microstructure of the infected
rice kernel showed that the outer dense chlamydospores
piled around the false smut balls grown on XBZ medium;
moreover the sclerotia consisting of dense mycelium were
found. The different morphology was observed across the
different growing conditions. In addition, we observed the
nuclear numbers of both the conidiospores and hyphae using
4′,6-diamidino-2-phenylindole (DAPI) staining. Because
the fungus has small chromosomes and the numbers were
not previously known, we analyzed the electrophoretic karyotype
using a pulsed field gel electrophoresis (PFGE) technique.
The results showed that V. virens has at least 10 chromosomes
ranging in size from 0.6 kb to 6 Mb. The V. virens
genome size is estimated to be 23 Mb. Here, we report the
morphological characteristics of the fungus and the process
of asexual spores forming asexual propagules, along with
the first analyze the molecular karyotype of V. virens. These
results
supply a foundation for further study of the pathogenicity
and biology of this devastating pathogen.
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Citations
Citations to this article as recorded by - Morpho-cultural and molecular characterization of Ustilaginoidea virens isolates collected from diverse geographical regions of India
Manas Kumar Bag, Prahlad Masurkar, Anuprita Ray, Amrita Banerjee
Indian Phytopathology.2026; 79(1): 37. CrossRef - Revitalizing the rice by using chemical fungicides and adjustment of date of sowing against false smut of rice disease
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Physiological and Molecular Plant Pathology.2025; 136: 102571. CrossRef - Exploring Ustilaginoidea virens, the causal agent of false smut of rice disease: A comprehensive study of infection dynamics, effectors, and genetic structure
Prahlad Masurkar, Jhumishree Meher, Sukram Thapa, R.K. Singh, M.K. Bag, Vedant Gautam, Shivam Maurya
Microbial Pathogenesis.2025; 208: 107987. CrossRef - From infection to resistance: A comprehensive review on false smut (Ustilaginoidea virens) and its impact on rice
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Physiological and Molecular Plant Pathology.2025; 139: 102758. CrossRef - Biological and molecular characterization of Ustilaginoidea virens, an emerging pathogen of rice in India
Sapna Sharma, Bishnu Maya Bashyal, Gaurav Kumar Yadav, Chetna Chugh, Sunil Kumar Sunani, Mohamad Ayham Shakouka, Ravish Choudhary, Slavica Matic, Ashish Kumar Gupta, Gopala Krishnan S.
Journal of Plant Pathology.2025; 107(4): 1863. CrossRef - Antifungal activity and inhibition of the molecular mechanism of lavender essential oil against Ustilaginoidea virens
Rongtao Fu, Liyu Zhao, Jian Wang, Cheng Chen, Yao Liu, Daihua Lu
LWT.2024; 203: 116315. CrossRef - Interaction Between Ustilaginoidea virens and Rice and Its Sustainable Control
Zhang Fengmin, Cao Zhenzhen, Zheng Xin, He Yuntao, Chen Mingxue, Lin Xiaoyan
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Jagjeet Singh Lore, Jyoti Jain, Sanjay Kumar, Ishwinder Kamboj, Navjot Sidhu, Renu Khanna, Rupinder Kaur, Gurjit Singh Mangat
Plant Genetic Resources: Characterization and Utilization.2022; 20(1): 7. CrossRef - Diversity Analysis of the Rice False Smut Pathogen Ustilaginoidea virens in Southwest China
Rongtao Fu, Cheng Chen, Jian Wang, Yao Liu, Liyu Zhao, Daihua Lu
Journal of Fungi.2022; 8(11): 1204. CrossRef - Improved methodology for the isolation of false smut pathogen Ustilaginoidea virens of rice
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Indian Phytopathology.2021; 74(1): 249. CrossRef - Ustilaginoidea virens: Insights into an Emerging Rice Pathogen
Wenxian Sun, Jing Fan, Anfei Fang, Yuejiao Li, Muhammad Tariqjaveed, Dayong Li, Dongwei Hu, Wen-Ming Wang
Annual Review of Phytopathology.2020; 58(1): 363. CrossRef - Development of a Low-Cost Narrow Band Multispectral Imaging System Coupled with Chemometric Analysis for Rapid Detection of Rice False Smut in Rice Seed
Haiyong Weng, Ya Tian, Na Wu, Xiaoling Li, Biyun Yang, Yiping Huang, Dapeng Ye, Renye Wu
Sensors.2020; 20(4): 1209. CrossRef - Development of rice conidiation media for Ustilaginoidea virens
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Mina Yu, Junjie Yu, Huijuan Cao, Mingli Yong, Yongfeng Liu
Genome.2019; 62(12): 807. CrossRef - Pathogenicity Genes in Ustilaginoidea virens Revealed by a Predicted Protein–Protein Interaction Network
Kang Zhang, Yuejiao Li, Tengjiao Li, Zhi-Gang Li, Tom Hsiang, Ziding Zhang, Wenxian Sun
Journal of Proteome Research.2017; 16(3): 1193. CrossRef - Rice false smut and its management in major rice growing areas in Ashanti region of Ghana
Dokie Tokpah Daniel, Kwoseh Charles, Saye Tokpah Eric, Kolleh David
African Journal of Agricultural Research.2017; 12(43): 3129. CrossRef - Current understanding on Villosiclava virens, a unique flower‐infecting fungus causing rice false smut disease
Jing Fan, Juan Yang, Yu‐Qiu Wang, Guo‐Bang Li, Yan Li, Fu Huang, Wen‐Ming Wang
Molecular Plant Pathology.2016; 17(9): 1321. CrossRef - Survey and analysis of simple sequence repeats in the Ustilaginoidea virens genome and the development of microsatellite markers
Mina Yu, Junjie Yu, Huanhuan Li, Yahui Wang, Xiaole Yin, Huiwen Bo, Hui Ding, Yuxin Zhou, Yongfeng Liu
Gene.2016; 585(1): 28. CrossRef - UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens
Dawei Zheng, Yi Wang, Yu Han, Jin-Rong Xu, Chenfang Wang
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Mina Yu, Junjie Yu, Jiankun Hu, Lei Huang, Yahui Wang, Xiaole Yin, Yafeng Nie, Xiangkun Meng, Weiduo Wang, Yongfeng Liu
Fungal Genetics and Biology.2015; 76: 10. CrossRef - Infection processes of Ustilaginoidea virens during artificial inoculation of rice panicles
Maolin Hu, Laixin Luo, Shu Wang, Yongfeng Liu, Jianqiang Li
European Journal of Plant Pathology.2014; 139(1): 67. CrossRef - <i>Ustilaginoidea virens</i> Infection of Rice in Arkansas: Toxicity of False Smut Galls, Their Extracts and the Ustiloxin Fraction
Hamed K. Abbas, Wayne Thomas Shier, Rick D. Cartwright, Gabe L. Sciumbato
American Journal of Plant Sciences.2014; 05(21): 3166. CrossRef - De novo sequencing and transcriptome analysis of Ustilaginoidea virens by using Illumina paired-end sequencing and development of simple sequence repeat markers
Mina Yu, Junjie Yu, Chenhao Gu, Yafeng Nie, Zhiyi Chen, Xiaole Yin, Yongfeng Liu
Gene.2014; 547(2): 202. CrossRef - Epiphytic colonization of Ustilaginoidea virens on biotic and abiotic surfaces implies the widespread presence of primary inoculum for rice false smut disease
J. Fan, X.‐Y. Guo, F. Huang, Y. Li, Y.‐F. Liu, L. Li, Y.‐J. Xu, J.‐Q. Zhao, H. Xiong, J.‐J. Yu, W. Wang
Plant Pathology.2014; 63(4): 937. CrossRef - Genetic Diversity and Population Structure of Rice Pathogen Ustilaginoidea virens in China
Xianyun Sun, Shu Kang, Yongjie Zhang, Xinqiu Tan, Yufei Yu, Haiyong He, Xinyu Zhang, Yongfeng Liu, Shu Wang, Wenxian Sun, Lei Cai, Shaojie Li, Vishnu Chaturvedi
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BMC Genomics.2013;[Epub] CrossRef
- Correlations of Fecal Bacterial Communities with Age and Living Region for the Elderly Living in Bama, Guangxi, China
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Liang Zhao , Xuewei Qiao , Jun Zhu , Xiaoying Zhang , Jingli Jiang , Yanling Hao , Fazheng Ren
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J. Microbiol. 2011;49(2):186-192. Published online May 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0405-x
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725
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0
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20
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Abstract
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Bama County (Guangxi, China) is famous for its longevous population. In this study, intestinal microflora of 17 healthy elderly subjects of different ages and from different regions (rural and urban) in Bama, were analyzed by denaturing gradient gel electrophoresis (DGGE). Significant effects of age and living region on the whole intestinal bacterial communities were observed by redundancy analysis (RDA). A total of 11 bacterial strains that were correlated with age and living region were identified using a t-value biplot combined with band sequencing. Four bacterial strains were correlated with both age and living region of the elderly in Bama. Two Bacteroides strains and one Ruminococcaceae strain were abundant in the
rural, younger elderly; conversely, one Desulfovibrio strain was high in the urban, older elderly. Another Bacteroidetes strain was only correlated with the participant’s age, and its abundance increased with the age of the elderly. The richness of one Clostridium sordellii strain, which was only correlated with the elderly living region, was high in the urban elderly. The study also found five other novel bacterial strains that were correlated with the age or living region of the elderly in Bama. These results expand our understanding of age- and region-effects on the intestinal microflora of the elderly and raise the possibility of developing probiotics originating from centenarians.
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