- Effects of tryptophan and phenylalanine on tryptophol production in Saccharomyces cerevisiae revealed by transcriptomic and metabolomic analyses
-
Xiaowei Gong , Huajun Luo , Liu Hong , Jun Wu , Heng Wu , Chunxia Song , Wei Zhao , Yi Han , Ya Dao , Xia Zhang , Donglai Zhu , Yiyong Luo
-
J. Microbiol. 2022;60(8):832-842. Published online May 27, 2022
-
DOI: https://doi.org/10.1007/s12275-022-2059-2
-
-
1,196
View
-
7
Download
-
13
Web of Science
-
13
Crossref
-
 Abstract
 PDF
-
Tryptophol (TOL) is a metabolic derivative of tryptophan
(Trp) and shows pleiotropic effects in humans, plants and
microbes. In this study, the effect of Trp and phenylalanine
(Phe) on TOL production in Saccharomyces cerevisiae was determined,
and a systematic interpretation of TOL accumulation
was offered by transcriptomic and metabolomic analyses.
Trp significantly promoted TOL production, but the output
plateaued (231.02−266.31 mg/L) at Trp concentrations ≥ 0.6
g/L. In contrast, Phe reduced the stimulatory effect of Trp,
which was strongly dependent on the Phe concentration. An
integrated genomic, transcriptomic, and metabolomic analysis
revealed that the effect of Trp and Phe on TOL production
was mainly related to the transamination and decarboxylation
of the Ehrlich pathway. Additionally, other genes, including
thiamine regulon genes (this), the allantoin catabolic
genes dal1, dal2, dal4, and the transcriptional activator gene
aro80, may play important roles. These findings were partly
supported by the fact that the thi4 gene was involved in TOL
production, as shown by heterologous expression analysis. To
the best of our knowledge, this novel biological function of thi4
in S. cerevisiae is reported here for the first time. Overall, our
findings provide insights into the mechanism of TOL production,
which will contribute to TOL production using metabolic
engineering strategies.
-
Citations
Citations to this article as recorded by  - Modular co-culture engineering of Escherichia coli and Saccharomyces cerevisiae for de novo biosynthesis of tryptophol from glucose
Zhenhao Fu, Jiamin Zheng, Lele Zhang, Jiansheng Jian, Zhonghu Bai, Ye Li
Bioresource Technology.2026; 449: 134398. CrossRef - An unfolded protein response-inducible protein (ULI1) mediates aromatic alcohols biosynthesis through reinforcing the Ehrlich pathway in Saccharomyces cerevisiae
Xiaowei Gong, Yong Shen, Shanzhai Shang, Jianbo Zhan, Guanghui Ma, Huajun Luo, Shiwei Li, Chunxia Song, Donglai Zhu, Yiyong Luo
Frontiers in Microbiology.2026;[Epub] CrossRef - Recent Advances
in Engineering Microbial Cell Factories
for Tryptophan-Derived Chemicals
Chenxi Qiu, Hongwei Guo, Quanpu Ren, Yujiao Sun, Ge Qu, Yongfen Wang, Ming Zheng, Zhoutong Sun
Journal of
Agricultural
and Food Chemistry.2026; 74(35): 27303. CrossRef - Strain-dependent modulation of selected Ehrlich pathway-associated genes during sequential fermentation with Metschnikowia pulcherrima and Saccharomyces cerevisiae
Elif Bircan Muyanlı, Remziye Yılmaz
European Food Research and Technology.2026;[Epub] CrossRef - The effect and mechanism of acetic acid -(1H-indole-3-ethyl) ester in controlling postharvest blue mold disease in apples
Xiangfeng Zheng, Qinghua Gong, Zhirong Wang, Peiwen Huang, Zihan Zhang, Lu Gao, Shengqi Rao, Zhenquan Yang
Postharvest Biology and Technology.2025; 227: 113618. CrossRef - Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering
Huimin Xue, Mingshan Li, Yuhui Cui, Dongkui Tian, Duo Liu, Hanjie Wang
Microbial Cell Factories.2025;[Epub] CrossRef - Lysine restriction attenuates obesity-related anxiety-like behaviors via increasing Oscillibacter ruminantium-mediated tryptophol regulation
Feng Zhao, Jiao Wang, Weijuan Liang, Fanwei Sun, Zhaoyi Liu, Yuehua Wu, Ahmad Khan, Chunsong Wang, Ruiqi Zhou, Huaili Feng, Ting You, Xinhao Duan, Hongyang Zhang, Jingfu Qiu, Chengzhi Chen
Microbiome.2025;[Epub] CrossRef - Nanostructured Biosensing Platforms for the Rapid Detection of Fungal Quorum-Sensing Molecules: Toward Early and Accurate Diagnosis of Candida albicans Infection
Ahmed M. Saleh, Omar Ramadan, Amr M. Badawey, Hoda M. Marzouk, Shuna Cui, Rabeay Y. A. Hassan
ACS Infectious Diseases.2025; 11(12): 3481. CrossRef - Human intestinal fungus Clavispora lusitaniae attenuates colitis through Pyruvate decarboxylase-derived Indole-3-ethanol
Fan Wu, Yan Wang, Zhenpeng Mai, Zhichao Xu, Shenghui Li, Yu Li, Ruiqiao Yin, Jiamin Li, Zhenlong Yu, Yuzhuo Wu, Xiangge Tian, Xiaoying Feng, Xiaokui Huo, Chao Wang, Xiaochi Ma
Nature Communications.2025;[Epub] CrossRef - Engineering the L-tryptophan metabolism for efficient de novo biosynthesis of tryptophol in Saccharomyces cerevisiae
Ye Li, Jingzhen Sun, Zhenhao Fu, Yubing He, Xiaorui Chen, Shijie Wang, Lele Zhang, Jiansheng Jian, Weihua Yang, Chunli Liu, Xiuxia Liu, Yankun Yang, Zhonghu Bai
Biotechnology for Biofuels and Bioproducts.2024;[Epub] CrossRef - Evaluating the Atypical Aging Potential Development in Sparkling Wines Can Be Achieved by Assessing the Base Wines at the End of the Alcoholic Fermentation
Simone Delaiti, Tiziana Nardin, Tomas Roman, Stefano Pedò, Roberto Larcher
Journal of Agricultural and Food Chemistry.2024; 72(9): 4918. CrossRef - Tryptophol Improves the Biocontrol Efficacy of Scheffersomyces spartinae against the Gray Mold of Strawberries by Quorum Sensing
Zichang Zhao, Yingying Wei, Xiurong Zou, Shu Jiang, Yi Chen, Jianfen Ye, Feng Xu, Hongfei Wang, Xingfeng Shao
Journal of Agricultural and Food Chemistry.2023; 71(49): 19739. CrossRef - A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisiae and Escherichia coli
Xinru Ren, Yue Wei, Honglu Zhao, Juanjuan Shao, Fanli Zeng, Zhen Wang, Li Li
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef
- Variation of cassiicolin genes among Chinese isolates of Corynespora cassiicola
-
Jun Wu , Xuewen Xie , Yanxia Shi , Ali Chai , Qi Wang , Baoju Li
-
J. Microbiol. 2018;56(9):691-691.
-
DOI: https://doi.org/10.1007/s12275-018-8580-7
-
-
853
View
-
0
Download
-
2
Crossref
-
Abstract
PDF
-
In the online published article by Wu et al. since 30 July 2018, the figure 4 is unfortunately incorrect. The figure 4 should be
corrected as below.
-
Citations
Citations to this article as recorded by - Genome sequence and spore germination-associated transcriptome analysis of Corynespora cassiicola from cucumber
Shigang Gao, Rong Zeng, Lihui Xu, Zhiwei Song, Ping Gao, Fuming Dai
BMC Microbiology.2020;[Epub] CrossRef - Endophytes from Wild Rubber Trees as Antagonists of the Pathogen Corynespora cassiicola
Valérie Pujade-Renaud, Marine Déon, Romina Gazis, Sébastien Ribeiro, Florence Dessailly, Françoise Granet, Priscila Chaverri
Phytopathology®.2019; 109(11): 1888. CrossRef
- Variation of cassiicolin genes among Chinese isolates of Corynespora cassiicola
-
Jun Wu , Xuewen Xie , Yanxia Shi , Ali Chai , Qi Wang , Baoju Li
-
J. Microbiol. 2018;56(9):634-647. Published online July 27, 2018
-
DOI: https://doi.org/10.1007/s12275-018-7497-5
-
-
1,009
View
-
2
Download
-
12
Crossref
-
Abstract
PDF
-
Corynespora cassiicola is a species of fungus that is a plant
pathogen of many agricultural crop plants, including severe
target spot disease on cucumber. Cassiicolin is an important
effector of pathogenicity of this fungus. In this study, we
collected 141 Corynespora isolates from eighteen hosts, and
the casscolin gene was detected in 82 C. cassiicola strains.
The deduced protein sequences revealed that 72 isolates
contained the Cas2 gene, two strains from Gynura bicolor
harboured the Cas2.2 gene, and 59 isolates without a cassiicolin
gene were classified as Cas0. Phylogenetic analyses was
performed for the 141 isolates using four loci (ITS, ga4, caa5,
and act1) and revealed two genetic clusters. Cluster A is composed
of four subclades: subcluster A1 includes all Cas2
isolates plus 18 Cas0 strains, subcluster A2 includes the eight
Cas5 isolates and one Cas0 isolate, and subclusters A3 and
A4 contain Cas0 strains. Cluster B consists of 21 Cas0 isolates.
Twenty-two C. cassiicola strains from different toxin
classes showed varying degrees of virulence against cucumber.
Cas0 or Cas2 strains induced diverse responses on cucumber,
from no symptoms to symptoms of moderate or severe
infection, but all Cas5 isolates exhibited avirulence on cucumber.
-
Citations
Citations to this article as recorded by - Diversity of cassiicolin profiles and culture filtrate toxicity of Corynespora cassiicola isolates from South Indian rubber plantations
Reshma T R, Shilpa Babu, Vineeth V K, Shaji Philip
Industrial Crops and Products.2025; 224: 120243. CrossRef - One stop shop V: taxonomic update with molecular phylogeny for important phytopathogenic genera: 101–125 (2024)
Ruvishika S. Jayawardena, Kevin D. Hyde, Herbert D. R. Aumentado, Pranami D. Abeywickarama, Shubhi Avasthi, Asha J. Dissanayake, Ajay K. Gautam, Achala J. Gajanayake, Alireza Armand, Mark A. O. Balendres, Mark S. Calabon, Yi Jyun Chen, K. W. Thilini Cheth
Fungal Diversity.2025; 130(1): 263. CrossRef - The Diseases and Pests of Rubber Tree and Their Natural Control Potential: A Bibliometric Analysis
Liqiong Chen, Lidan Xu, Xiaona Li, Yilin Wang, Yun Feng, Guixiu Huang
Agronomy.2023; 13(8): 1965. CrossRef - Need for disease resistance breeding against Corynespora cassiicola in crops
Edgar Sierra-Orozco, German Sandoya, Seonghee Lee, Gary Vallad, Samuel Hutton
Frontiers in Agronomy.2023;[Epub] CrossRef - Comparison and Correlation of Corynespora cassiicola Populations from Kiwifruit and Other Hosts Based on Morphology, Phylogeny, and Pathogenicity
Jing Xu, Guoshu Gong, Yongliang Cui, Yuhang Zhu, Jun Wang, Kaikai Yao, Wen Chen, Cuiping Wu, Rui Yang, Xiaodan Yang, Pan Li, Henan Zhao, Sen Zhong, Yi Luo, Yue Li, Wenfei Liao
Plant Disease.2023; 107(7): 1979. CrossRef - Unraveling the Host-Selective Toxic Interaction of Cassiicolin with Lipid Membranes and Its Cytotoxicity
Kien Xuan Ngo, Phuong Doan N. Nguyen, Hirotoshi Furusho, Makoto Miyata, Tomomi Shimonaka, Nguyen Ngoc Bao Chau, Nguyen Phuong Vinh, Nguyen Anh Nghia, Tareg Omer Mohammed, Takehiko Ichikawa, Noriyuki Kodera, Hiroki Konno, Takeshi Fukuma, Nguyen Bao Quoc
Phytopathology®.2022; 112(7): 1524. CrossRef - The necrosis- and ethylene-inducing peptide 1-like protein (NLP) gene family of the plant pathogen Corynespora cassiicola
Thaís Carolina da Silva Dal’Sasso, Vinícius Delgado da Rocha, Hugo Vianna Silva Rody, Maximiller Dal-Bianco Lamas Costa, Luiz Orlando de Oliveira
Current Genetics.2022; 68(5-6): 645. CrossRef - Identification and virulence evaluation of Corynespora cassiicola cassiicolin-encoding gene isolates from rubber trees in Vietnam
Nguyen Ngoc Bao Chau, Nguyen Van Minh, Nguyen Mai Nghiep, Nguyen Phuong Vinh, Nguyen Anh Nghia, Nguyen Bao Quoc
Tropical Plant Pathology.2022; 47(3): 378. CrossRef - The fungal pathogen Corynespora cassiicola: A review and insights for target spot management on cotton and Soya bean
Marina N. Rondon, Kathy Lawrence
Journal of Phytopathology.2021; 169(6): 329. CrossRef -
Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen
Corynespora cassiicola
Qingzhou Ma, Haiyan Wu, Yuehua Geng, Qiang Li, Rui Zang, Yashuang Guo, Chao Xu, Meng Zhang
Computational and Structural Biotechnology Journal.2021; 19: 5987. CrossRef - Genomic Characteristics and Comparative Genomics Analysis of Two Chinese Corynespora cassiicola Strains Causing Corynespora Leaf Fall (CLF) Disease
Boxun Li, Yang Yang, Jimiao Cai, Xianbao Liu, Tao Shi, Chaoping Li, Yipeng Chen, Pan Xu, Guixiu Huang
Journal of Fungi.2021; 7(6): 485. CrossRef - Endophytes from Wild Rubber Trees as Antagonists of the Pathogen Corynespora cassiicola
Valérie Pujade-Renaud, Marine Déon, Romina Gazis, Sébastien Ribeiro, Florence Dessailly, Françoise Granet, Priscila Chaverri
Phytopathology®.2019; 109(11): 1888. CrossRef
|
MSK
