- Effects of rehydration on physiological and transcriptional responses of a water-stressed rhizobium
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Jie Zhu , Xin Jiang , Dawei Guan , Yaowei Kang , Li Li , Fengming Cao , Baisuo Zhao , Mingchao Ma , Ji Zhao , Jun Li
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J. Microbiol. 2022;60(1):31-46. Published online November 26, 2021
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DOI: https://doi.org/10.1007/s12275-022-1325-7
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 Abstract
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As a microsymbiont of soybean, Bradyrhizobium japonicum
plays an important role in symbiotic nitrogen fixation and
sustainable agriculture. However, the survival of B. japonicum
cells under water-deplete (e.g., drought) and water-replete
(e.g., flood) conditions is a major concern affecting their
nitrogen-fixing ability by establishing the symbiotic relationship
with the host. In this study, we isolated a water stress tolerant
rhizobium from soybean root nodules and tested its
survival under water-deplete conditions. The rhizobium was
identified as Bradyrhizobium japonicum and named strain
5038. Interestingly, both plate counting and live/dead fluorescence
staining assays indicate that a number of viable but
non-culturable cells exist in the culture medium upon the rehydration
process which could cause dilution stress. Bradyrhizobium
japonicum 5038 cells increased production of exopolysaccharide
(EPS) and trehalose when dehydrated, suggesting
that protective responses were stimulated. As expected,
cells reduced their production upon the subsequent rehydration.
To examine differential gene expression of B. japonicum
5038 when exposed to water-deplete and subsequent waterreplete
conditions, whole-genome transcriptional analysis was
performed under 10% relative humidity (RH), and subsequent
100% RH, respectively. A total of 462 differentially expressed
genes (DEGs, > 2.0-fold) were identified under the 10% RH
condition, while 3,776 genes showed differential expression
during the subsequent rehydration (100% RH) process. Genes
involved in signal transduction, inorganic ion transport, energy
production and metabolisms of carbohydrates, amino
acids, and lipids were far more up-regulated than downregulated
in the 10% RH condition. Notably, trehalose biosynthetic
genes (otsAB, treS, and treYZ), genes ligD, oprB, and
a sigma factor rpoH were significantly induced by 10% RH.
Under the subsequent 100% RH condition, genes involved in
transcription, translation, cell membrane regulation, replication
and repair, and protein processing were highly up-regulated.
Interestingly, most of 10%-RH inducible genes displayed
rehydration-repressed, except three genes encoding heat shock
(Hsp20) proteins. Therefore, this study provides molecular
evidence for the switch of gene expression of B. japonicum
cells when encountered the opposite water availability from
water-deplete to water-replete conditions.
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Citations
Citations to this article as recorded by  - Microbial survival strategies in desiccated roots of Myrothamnus flabellifolia
Shandry M. Tebele, Rose A. Marks, Jill M. Farrant
Frontiers in Microbiology.2025;[Epub] CrossRef - Drought-induced plant microbiome and metabolic enrichments improve drought resistance
Jiayu Li, Hongwei Liu, Juntao Wang, Catriona A. Macdonald, Pankaj Singh, Vu Thanh Cong, Marcus Klein, Manuel Delgado-Baquerizo, Brajesh K. Singh
Cell Host & Microbe.2025; 33(6): 882. CrossRef - Challenges to rhizobial adaptability in a changing climate: Genetic engineering solutions for stress tolerance
Yunjia Zhang, Yee-Shan Ku, Tsz-Yan Cheung, Sau-Shan Cheng, Dawei Xin, Kewin Gombeau, Yizhi Cai, Hon-Ming Lam, Ting-Fung Chan
Microbiological Research.2024; 288: 127886. CrossRef - Plant–Soil Microbial Interaction: Differential Adaptations of Beneficial vs. Pathogenic Bacterial and Fungal Communities to Climate-Induced Drought
Nataliya Loiko, M. Islam
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Huimin Ju, Jian Zhang, Yiyang Zou, Feiyang Xie, Xiaoyu Tang, Si Zhang, Jie Li
Environmental Research.2024; 250: 118469. CrossRef - Effect of Nitrogen Application and Cutting Frequency on the Yield and Forage Quality of Alfalfa in Seasonal Cultivation
Kun Zhang, Chenyuan Zhai, Yonglong Li, Yan Li, Hui Qu, Yixin Shen
Agriculture.2023; 13(5): 1063. CrossRef - Profound Change in Soil Microbial Assembly Process and Co-occurrence Pattern in Co-inoculation of Bradyrhizobium japonicum 5038 and Bacillus aryabhattai MB35-5 on Soybean
Yubin Zhao, Dawei Guan, Xu Liu, Gui-Feng Gao, Fangang Meng, Bingqiang Liu, Pengfei Xing, Xin Jiang, Mingchao Ma, Fengming Cao, Li Li, Jun Li
Frontiers in Microbiology.2022;[Epub] CrossRef
- Detection of Pseudomonas aeruginosa Carried a New Array of Gene Cassettes within Class 1 Integron Isolated from a Teaching Hospital in Nanjing, China
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Yuan Wu† , Hui Li† , Jun Li , Zu Hu Huang
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J. Microbiol. 2008;46(6):687-691. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0021-6
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804
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We report here novel array of gene cassettes found in single variable region of class 1 integron disseminated in Pseudomonas aeruginosa isolated from a teaching hospital in Nanjing, Jiangsu Province, China. 29 of 47 (61%) P. aeruginosa strains were confirmed haboured class 1 integron, and all the positive strains have the same variable region confirmed by PCR and RFLP methods. The variable region contained an unreported order of four gene cassettes aac(6’)-II-aadA13-cmlA8-oxa 10. Of those, cmlA8 gene was a variant of cmlA5 encoding non-enzymatic protein which putatively confer resistance to chloramphenicol. Susceptibility testing revealed multidrug-resistant mechanisms were involved in the class 1 integron positive clinical isolates. And the class 1 integron located on an about 15 kb transferable plasmid was certified by conjugation experiment and plasmid DNA analysis. The macro restriction profile indicated those clinical strains were clonally related.
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