Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Browse Articles > Author index
Search
Joong-Kook Choi 1 Article
Antibacterial Activity of Recombinant hCAP18/LL37 Protein Secreted from Pichia pastoris
Soon-ja Kim , Renshu Quan , Sung-Jin Lee , Hak-Kyo Lee , Joong-Kook Choi
J. Microbiol. 2009;47(3):358-362.   Published online June 26, 2009
DOI: https://doi.org/10.1007/s12275-009-0131-9
  • 858 View
  • 1 Download
  • 14 Crossref
AbstractAbstract PDF
Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZαA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.

Citations

Citations to this article as recorded by  
  • Bacterial contamination in starch-to-ethanol fermentations: Can bacteriocin-producing Saccharomyces cerevisiae offer a solution?
    Michelle Rossouw, Bianca J Campbell, Rosemary A Cripwell, Leon M T Dicks, Marinda Viljoen-Bloom
    Journal of Industrial Microbiology and Biotechnology.2026;[Epub]     CrossRef
  • Antimicrobial Peptides: Mechanisms, Applications, and Therapeutic Potential
    Mohammed Alzain, Hussam Daghistani, Taghreed Shamrani, Yousef Almoghrabi, Yassir Daghistani, Ohood Alharbi, Ahmad Sait, Mohammed Mufrrih, Wafaa Alhazmi, Mona Alqarni, Bandar Saleh, Manal Zubair, Noha Juma, Hatoon Niyazi, Hanouf Niyazi, Waiel Halabi, Rawan
    Infection and Drug Resistance.2025; Volume 18: 4385.     CrossRef
  • Unveiling the Novel Benefits of Co-Administering Butyrate and Active Vitamin D3 in Mice Subjected to Chemotherapy-Induced Gut-Derived Pseudomonas aeruginosa Sepsis
    Fu-Chen Huang, Shun-Chen Huang
    Biomedicines.2024; 12(5): 1026.     CrossRef
  • Evaluation of Novel HLM Peptide Activity and Toxicity against Planktonic and Biofilm Bacteria: Comparison to Standard Antibiotics
    Majed M. Masadeh, Haneen Alshogran, Mohammad Alsaggar, Salsabeel H. Sabi, Enaam M. Al Momany, Majd M. Masadeh, Nasr Alrabadi, Karem H. Alzoubi
    Current Protein & Peptide Science.2024; 25(10): 826.     CrossRef
  • Expression, purification and investigation of antibacterial activity of a novel hybrid peptide LL37/hBD-129 by applied comprehensive computational and experimental approaches
    Mahsa Abbasi, Esmail Behmard, Mohammad Hashem Yousefi, Seyed Shahram Shekarforoush, Shirin Mahmoodi
    Archives of Microbiology.2023;[Epub]     CrossRef
  • Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds
    Mine Erdem Büyükkiraz, Zülal Kesmen
    Journal of Applied Microbiology.2022; 132(3): 1573.     CrossRef
  • Heterologous Expression and Functional Characterization of CAP18 from Oryctolagus cuniculus
    Mahla Nikpoor, Azadeh Lohrasbi-Nejad, Jafar Zolala
    Reports of Biochemistry and Molecular Biology.2022; 10(4): 622.     CrossRef
  • Peroxisome-targeted and tandem repeat multimer expressions of human antimicrobial peptide LL37 in Pichia pastoris
    Siwei Xiao, Yanyun Gao, Xiaolong Wang, Wei Shen, Jinjia Wang, Xiangshan Zhou, Menghao Cai, Yuanxing Zhang
    Preparative Biochemistry & Biotechnology.2017; 47(3): 229.     CrossRef
  • Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
    Kelly Cristina Mulder, Loiane Alves de Lima, Priscilla Santos Aguiar, Fábio Correa Carneiro, Octávio Luiz Franco, Simoni Campos Dias, Nádia Skorupa Parachin
    AMB Express.2015;[Epub]     CrossRef
  • Cathelicidin-like Helminth Defence Molecules (HDMs): Absence of Cytotoxic, Anti-microbial and Anti-protozoan Activities Imply a Specific Adaptation to Immune Modulation
    Karine Thivierge, Sophie Cotton, Deborah A. Schaefer, Michael W. Riggs, Joyce To, Maria E. Lund, Mark W. Robinson, John P. Dalton, Sheila M. Donnelly, Edward Mitre
    PLoS Neglected Tropical Diseases.2013; 7(7): e2307.     CrossRef
  • A comprehensive summary of LL-37, the factotum human cathelicidin peptide
    Dieter Vandamme, Bart Landuyt, Walter Luyten, Liliane Schoofs
    Cellular Immunology.2012; 280(1): 22.     CrossRef
  • Expression systems for heterologous production of antimicrobial peptides
    Nádia Skorupa Parachin, Kelly Cristina Mulder, Antônio Américo Barbosa Viana, Simoni Campos Dias, Octávio Luiz Franco
    Peptides.2012; 38(2): 446.     CrossRef
  • Recombinant antimicrobial peptide hPAB-β expressed in Pichia pastoris, a potential agent active against methicillin-resistant Staphylococcus aureus
    Zhijin Chen, Dongmei Wang, Yanguang Cong, Jing Wang, Junmin Zhu, Jie Yang, Zhen Hu, Xiaomei Hu, Yinling Tan, Fuquan Hu, Xiancai Rao
    Applied Microbiology and Biotechnology.2011; 89(2): 281.     CrossRef
  • Design, Recombinant Expression, and Antibacterial Activity of the Cecropins–Melittin Hybrid Antimicrobial Peptides
    Yu Cao, Rong Qing Yu, Yi Liu, Huo Xiang Zhou, Ling Ling Song, Yi Cao, Dai Rong Qiao
    Current Microbiology.2010; 61(3): 169.     CrossRef

Journal of Microbiology : Journal of Microbiology
TOP