- Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation
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Seo-jeong Park , Joon Woo Ahn , Jong-il Choi
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J. Microbiol. 2022;60(1):63-69. Published online December 29, 2021
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DOI: https://doi.org/10.1007/s12275-022-1491-7
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 Abstract
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In a previous study, a putative 2-amino-3-carboxymuconate-
6-semialdehyde decarboxylase (ACMSD) was highly expressed
in a mutant strain of Pyropia yezoensis, which exhibited an
improved growth rate compared to its wild strain. To investigate
the functional role of the putative ACMSD (Pyacmsd)
of P. yezoensis, the putative Pyacmsd was cloned and expressed
in Chlamydomonas reinhardtii. Recombinant C. reinhardtii
cells with Pyacmsd (Cr_Pyacmsd) exhibited enhanced tolerance
compared to control C. reinhardtii cells (Cr_control)
under nitrogen starvation. Notably, Cr_Pyacmsd cells showed
accumulation of lipids in nitrogen-enriched conditions. These
results
demonstrate the role of Pyacmsd in the generation of
acetyl-coenzyme A. Thus, it can be used to enhance the production
of biofuel using microalgae such as C. reinhardtii and
increase the tolerance of other biological systems to nitrogendeficient
conditions.
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Citations
Citations to this article as recorded by  - Recent advances in Chlamydomonas reinhardtii as a green factory for sustainable biofuels and bioproduct production
Joon-Woo Ahn, Jong-il Choi
New Biotechnology.2026; 93: 362. CrossRef - Characteristics of Recombinant Chlamydomonas reinhardtii Expressing Putative Germin-Like Protein from Neopyropia yezoensis
Jiae Kim, Jong-il Choi
Journal of Microbiology and Biotechnology.2024; 34(10): 2132. CrossRef - Overexpression of putative glutathione peroxidase from Neopyropia-associated microorganisms in Chlamydomonas to respond to abiotic stress
Jeong Hyeon Kim, Eun-Jeong Park, Jong-il Choi
Archives of Microbiology.2023;[Epub] CrossRef - Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production
Jeong Hyeon Kim, Joon Woo Ahn, Eun-Jeong Park, Jong-il Choi
Journal of Microbiology and Biotechnology.2023; 33(3): 310. CrossRef
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