- LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe
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Won-Hwa Kang , Yoon-Dong Park , Joo-Yeon Lim , Hee-Moon Park
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J. Microbiol. 2024;62(1):21-31. Published online January 5, 2024
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DOI: https://doi.org/10.1007/s12275-023-00097-7
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It was reported that LAMMER kinase in Schizosaccharomyces pombe plays an important role in cation-dependent and
galactose-specific flocculation. Analogous to other flocculating yeasts, when cell wall extracts of the Δlkh1 strain were treated
to the wild-type strain, it displayed flocculation. Gas2, a 1,3-β-glucanosyl transferase, was isolated from the EDTA-extracted
cell-surface proteins in the Δlkh1 strain. While disruption of the gas2+ gene was not lethal and reduced the flocculation
activity of the Δlkh1 strain, the expression of a secreted form of Gas2, in which the GPI anchor addition sequences had been
removed, conferred the ability to flocculate upon the WT strain. The Gas2-mediated flocculation was strongly inhibited by
galactose but not by glucose. Immunostaining analysis showed that the cell surface localization of Gas2 was crucial for the
flocculation of fission yeast. In addition, we identified the regulation of mbx2+ expression by Lkh1 using RT-qPCR. Taken
together, we found that Lkh1 induces asexual flocculation by regulating not only the localization of Gas2 but also the transcription
of gas2+ through Mbx2.
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Citations
Citations to this article as recorded by  - Genetic and environmental determinants of multicellular-like phenotypes in fission yeast
Bence Kӧvér, Céleste E Cohen, Ladislav Seres, Saniya Raut, Markus Ralser, Benjamin M Heineike, Jürg Bähler, P Wittkopp
GENETICS.2026;[Epub] CrossRef
- Expression of sexual genes in Aspergillus fumigatus homogeneous culture produced by vegetative mass mating
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Joo-Yeon Lim , Hee-Moon Park
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J. Microbiol. 2019;57(8):688-693. Published online May 11, 2019
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DOI: https://doi.org/10.1007/s12275-019-9094-7
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Abstract
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There are presently no studies on the genes for sexual development
of Aspergillus fumigatus in situ using mating culture,
primarily because of challenging experimental conditions
that require a significantly long period of induction and produce
developmentally heterogenous culture, harboring very
few sexual organs. In order to overcome these challenges, we
developed an efficient and convenient procedure called ‘vegetative
mass mating (VeM)’ for study at a molecular level.
The VeM method enabled production of a developmentally
homogenous A. fumigatus culture, harboring many sexual
organs in a plate within a short period of two weeks. Feasibility
of the use of VeM for functional study of genes during
A. fumigatus sexual development was evaluated by analyzing
the transcription pattern of genes involved in pheromone signal
transduction and regulation of sexual development. Here,
we present for the first time, an in situ expression pattern of
sexual genes during the mating process, induced by the VeM
method
, which will enable and promote the sexual development
study of A. fumigatus at the molecular level.
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Citations
Citations to this article as recorded by - The Gβ-like Protein AfCpcB Affects Sexual Development, Response to Oxidative Stress and Phagocytosis by Alveolar Macrophages in Aspergillus fumigatus
Joo-Yeon Lim, Yeon-Ju Kim, Hee-Moon Park
Journal of Fungi.2022; 8(1): 56. CrossRef - The LAMMER Kinase, LkhA, Affects Aspergillus fumigatus Pathogenicity by Modulating Reproduction and Biosynthesis of Cell Wall PAMPs
Joo-Yeon Lim, Yeon Ju Kim, Seul Ah Woo, Jae Wan Jeong, Yu-Ri Lee, Cheol-Hee Kim, Hee-Moon Park
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Global Sexual Fertility in the Opportunistic Pathogen Aspergillus fumigatus and Identification of New Supermater Strains
Sameira S. Swilaiman, Céline M. O’Gorman, Wenyue Du, Janyce A. Sugui, Joanne Del Buono, Matthias Brock, Kyung J. Kwon-Chung, George Szakacs, Paul S. Dyer
Journal of Fungi.2020; 6(4): 258. CrossRef
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