Journal of Microbiology

Jong Yul Roh

8 Articles

Functional Characterization of Autographa californica Multiple Nucleopolyhedrovirus ORF43 and Phenotypic Changes of ORF43-Knockout Mutant: Xue Ying Tao , Jae Young Choi , Yong Wang , Jong Yul Roh , Joo Hyun Lee , Qin Liu , Jong Bin Park , Jae Su Kim , Woojin Kim , Yeon Ho Je; J. Microbiol. 2013;51(4):515-521. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3058-0

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ORF43 (ac43) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene of unknown function. To investigate the role of ac43 in the baculovirus lifecycle, we constructed an ac43-deleted mutant AcMNPV, Ac43KO. After transfection into Spodoptera frugiperda cells, Ac43KO produced polyhedra much larger in size than those of wild-type AcMNPV. Interestingly, some of the nucleocapsids were singly enveloped in the polyhedrin matrix while the nucleocapsids of AcMNPV are known to be multiply enveloped. Furthermore, Ac43KO led to a defect in the transcription and expression of polyhedrin, which resulted in reduced occlusion body production. However, Ac43KO did not affect production of budded virus as there was no remarkable difference in budded virus titer. These results suggest that ac43 plays an important role in the expression of polyhedrin, the morphogenesis of occlusion body, and the assembly of virions occluded in occlusion bodies.

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Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review
Ximei Yuan, Chen Wang, Yiqing He, Yuejun Fu
Biotechnology Advances.2025; 83: 108627. CrossRef
Successful Rescue of Synthetic AcMNPV with a ~17 kb Deletion in the C1 Region of the Genome
Yijia Guo, Hengrui Hu, Han Xiao, Fei Deng, Jiang Li, Manli Wang, Zhihong Hu
Viruses.2022; 14(12): 2780. CrossRef
Systematic analysis of nuclear localization of Autographa californica multiple nucleopolyhedrovirus proteins
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Journal of General Virology.2021;[Epub] CrossRef
The autographa californica multiple nucleopolyhedrovirus Ac12: A non-essential F box-like protein that interacts with cellular SKP1 component of the E3 ubiquitin ligase complex
Guadalupe S. Costa Navarro, Sabrina Amalfi, María Gabriela López, Gabriela Llauger, Joel D. Arneodo, Oscar Taboga, Victoria Alfonso
Virus Research.2019; 260: 67. CrossRef
Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins
Zhong-Jian Guo, Meng-Han Yu, Xian-Yun Dong, Wei-Li Wang, Ting Tian, Xian-Yin Yu, Xu-Dong Tang
Scientific Reports.2017;[Epub] CrossRef
Disruption of the baculovirus core gene ac78 results in decreased production of multiple nucleocapsid-enveloped occlusion-derived virions and the failure of primary infection in vivo
Sai-Nan Li, Jin-Yu Wang, Mei-Jin Yuan, Kai Yang
Virus Research.2014; 191: 70. CrossRef
The Trichoplusia ni single nucleopolyhedrovirus tn79 gene encodes a functional sulfhydryl oxidase enzyme that is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the sulfhydryl oxidase ac92 gene
Stian A. Clem, Wenbi Wu, A. Lorena Passarelli
Virology.2014; 460-461: 207. CrossRef
Baculovirus nucleocapsid aggregation (MNPV vs SNPV): an evolutionary strategy, or a product of replication conditions?
George F. Rohrmann
Virus Genes.2014; 49(3): 351. CrossRef

Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum: Qin Liu , Jong Yul Roh , Yong Wang , Jae Young Choi , Xue Ying Tao , Jae Su Kim , Yeon Ho Je; J. Microbiol. 2012;50(5):874-877. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2201-7

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A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.

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Research progress on the microbiota in bladder cancer tumors
Keyuan Lou, Junpeng Chi, Jitao Wu, Jian Ma, Shu Liu, Yuanshan Cui
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Characterization of microbial communities in urban subway: connotation for indoor environment quality and public health
Yongping Liu, Lijun Zhang, Duo Wang, Yewen Shi, Ling Tong, Feier Chen, Xiaojing Li, Chunyang Dong, Jianghua Zhang
Air Quality, Atmosphere & Health.2024; 17(7): 1401. CrossRef
Taxonomic hierarchy of the phylum Firmicutes and novel Firmicutes species originated from various environments in Korea
Chi Nam Seong, Joo Won Kang, Ji Hee Lee, So Yeon Seo, Jung Jae Woo, Chul Park, Kyung Sook Bae, Mi Sun Kim
Journal of Microbiology.2018; 56(1): 1. CrossRef
Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria
Zahia Djenane, Farida Nateche, Meriam Amziane, Joaquín Gomis-Cebolla, Fairouz El-Aichar, Hassiba Khorf, Juan Ferré
Toxins.2017; 9(4): 139. CrossRef

Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1: Ming Shun Li , Jong Yul Roh , Xueying Tao , Zi Niu Yu , Zi Duo Liu , Qin Liu , Hong Guang Xu , Hee Jin Shim , Yang-Su Kim , Yong Wang , Jae Young Choi , Yeon Ho Je; J. Microbiol. 2009;47(4):466-472. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0020-2

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Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8~25.2%) with the Rep protein coded by RCR plasmids, however. The putative double- strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. thuringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

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Staphylococcus equorum plasmid pKS1030-3 encodes auxiliary biofilm formation and trans-acting gene mobilization systems
Sojeong Heo, Seung-Eun Oh, Gawon Lee, Jinwook Lee, Nam-Chul Ha, Che Ok Jeon, Keuncheol Jeong, Jong-Hoon Lee, Do-Won Jeong
Scientific Reports.2023;[Epub] CrossRef
Transfer of a mobile Staphylococcus saprophyticus plasmid isolated from fermented seafood that confers tetracycline resistance
Jong-Hoon Lee, Sojeong Heo, Miran Jeong, Do-Won Jeong, Kyoung Heon Kim
PLOS ONE.2019; 14(2): e0213289. CrossRef
Risks for public health related to the presence of Bacillus cereus and other Bacillus spp. including Bacillus thuringiensis in foodstuffs

EFSA Journal.2016;[Epub] CrossRef
Characterization of Mobile Staphylococcus equorum Plasmids Isolated from Fermented Seafood That Confer Lincomycin Resistance
Jong-Hoon Lee, Do-Won Jeong, Ulrich Nübel
PLOS ONE.2015; 10(10): e0140190. CrossRef

Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses: Hyun Na Koo , Jeong Mi Oh , Jae Kyung Lee , Jae Young Choi , Kwang Sik Lee , Jong Yul Roh , Yeon Ho Je , Byung Rae Jin , Sung Sik Yoo , Jae Su Kim , Young In Kim , In Joong Yoon , Soo Dong Woo; J. Microbiol. 2008;46(6):709-719. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0224-x

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To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.

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Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016
Hyeonjeong Kang, Ji Eun Yu, Ji-Eun Shin, Areum Kang, Won-Il Kim, Changhee Lee, Jienny Lee, In-Soo Cho, Se-Eun Choe, Sang-Ho Cha
BMC Veterinary Research.2018;[Epub] CrossRef
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Hyun Na Koo, Sung Min Bae, Soo Dong Woo
International Journal of Industrial Entomology.2016; 32(2): 90. CrossRef
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Baochao Fan, Xing Liu, Juan Bai, Yufeng Li, Qiaoya Zhang, Ping Jiang, Guihong Zhang
PLOS ONE.2015; 10(9): e0138772. CrossRef
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Hwan-Won Choi, Eeuri Nam, Yoo Jin Lee, Yun-Hee Noh, Seung-Chul Lee, In-Joong Yoon, Hyun-Soo Kim, Shien-Young Kang, Young-Ki Choi, Changhee Lee
Veterinary Microbiology.2014; 170(3-4): 232. CrossRef
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Journal of Bacteriology and Virology.2011; 41(3): 173. CrossRef

Molecular and Phylogenetic Characterization of Spodoptera litura Granulovirus: Yong Wang , Jae Young Choi , Jong Yul Roh , Soo Dong Woo , Byung Rae Jin , Yeon Ho Je; J. Microbiol. 2008;46(6):704-708. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0133-z

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Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240~340 nm×140~180 nm. Each granule contained one single rod-shape virion with a mean size of 180~200 nm×20~40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus.

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María Leticia Ferrelli, Ricardo Salvador, Marina Elizabeth Biedma, Marcelo Facundo Berretta, Santiago Haase, Alicia Sciocco-Cap, Pablo Daniel Ghiringhelli, Víctor Romanowski
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Propagation of Bombyx mori Nucleopolyhedrovirus in Nonpermissive Insect Cell Lines: Soo-Dong Woo , Jong Yul Roh , Jae Young Choi , Byung Rae Jin; J. Microbiol. 2007;45(2):133-138.; DOI: https://doi.org/2522 [pii]

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Abstract PDF: This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via β-galactosidase expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

Expression of a Recombinant Cry1Ac Crystal Protein Fused with a Green Fluorescent Protein in Bacillus thuringiensis subsp. kurstaki Cry-B: Jong Yul Roh , In Hee Lee , Ming Shun Li , Jin Hee Chang , Jae Young Choi , Kyung Saeng Boo , Yeon Ho Je; J. Microbiol. 2004;42(4):340-345.; DOI: https://doi.org/2101 [pii]

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Abstract PDF: To investigate the co-expression and crystallization of a fusion gene between the Bacillus thuringiensis crystal protein and a foreign protein in B. thuringiensis, the expression of the Cry1Ac fused with green fluorescent protein (GFP) genes in a B. thuringiensis Cry-B strain was examined. The cry1Ac gene was cloned in the B. thuringiensis-E. coli shuttle vector, pHT3101, under the control of the native cry1Ac gene promoter, while the GFP gene was inserted into the XhoI site upstream of the proteolytic cleavage site, in the middle region of the cry1Ac gene (pProAc-GFP). The B. thuringiensis Cry-B strain carrying pProAc-GFP (ProAc-GFP/CB) did not produce any inclusion bodies. However, the transformed strain expressed fusion protein forms although the expression level was relatively low. Furthermore, an immunoblot analysis using GFP and Cry1Ac antibodies showed that the fusion protein was not a single species, but rather multiple forms. In addition, the N-terminal fragment of Cry1Ac and a non-fused GFP were also found in the B. thuringiensis Cry-B strain after autolysis. The sporulated cells before autolysis and the spore-crystal mixture after autolysis of ProAc-GFP/CB exhibited insecticidal activities against Plutella xylostella larvae. Accordingly, the current results suggest that a fusion crystal protein produced by the transfomant, ProAc-GFP/CB, can be functionally expressed but easily degraded in B. thuringiensis.

Isolation and Characterization of a Lymantria dispar Multinucleocapsid Nucleopolyhedrovirus Isolate in Korea: Hee Jin Shim , Jong Yul Roh , Jae Young Choi , Ming Shun Li , Soo Dong Woo , Hyun Woo Oh , Kyung Saeng Boo , Yeon Ho Je; J. Microbiol. 2003;41(4):306-311.

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Abstract PDF: In Korea, a Lymantria dispar multinucleocapsid nucleopolyhedrovirus, LdMNPV-NM, was isolated and characterized from dead L. dispar larvae. The polyhedra of LdMNPV-NM were irregularly shaped with a diameter of 1.62?0.33 ?m. Numerous virions comprised of the multinucleocapsid were evident in the electron microscopic examination of the polyhedra cross sections. These polyhedra were composed of a major protein of 30 kDa. The restriction enzyme digestion patterns of LdMNPV-NM showed that this isolate had some different fragments from those of the Gypchek팜?LdMNPV isolate, although their overall profiles were similar. The deduced amino acid sequence of the enhancin gene of LdMNPV-NM showed differences when compared to previously reported enhancin genes of other LdMNPV strains. These results suggested that the LdMNPV-NM isolate from Korea was a new NPV strain and had a new enhancin gene.

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