Journal of Microbiology

Joao Lucio Azevedo

6 Articles

Transmission of Methylobacterium mesophilicum by Bucephalogonia xanthophis for Paratransgenic Control Strategy of Citrus Variegated Chlorosis: Cláudia Santos Gai , Paulo Teixeira Lacava , Maria Carolina Quecine , Marie-Christine Auriac , João Roberto Spotti Lopes , Welington Luiz Araújo , Thomas Albert Miller , João Lúcio Azevedo; J. Microbiol. 2009;47(4):448-454. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-008-0303-z

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Methylobacterium mesophilicum, originally isolated as an endophytic bacterium from citrus plants, was genetically transformed to express green fluorescent protein (GFP). The GFP-labeled strain of M. mesophilicum was inoculated into Catharanthus roseus (model plant) seedlings and further observed colonizing its xylem vessels. The transmission of this endophyte by Bucephalogonia xanthophis, one of the insect vectors that transmit Xylella fastidiosa subsp. pauca, was verified by insects feeding from fluids containing the GFP bacterium followed by transmission to plants and isolating the endophyte from C. roseus plants. Forty-five days after inoculation, the plants exhibited endophytic colonization by M. mesophilicum, confirming this bacterium as a nonpathogenic, xylem-associated endophyte. Our data demonstrate that M. mesophilicum not only occupy the same niche of X. fastidiosa subsp. pauca inside plants but also may be transmitted by B. xanthophis. The transmission, colonization, and genetic manipulation of M. mesophilicum is a prerequisite to examining the potential use of symbiotic control to interrupt the transmission of X. fastidiosa subsp. pauca, the bacterial pathogen causing Citrus variegated chlorosis by insect vectors.

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Xylem endophytes of Salicaceae: potential role in mitigating disease symptoms from Xylella fastidiosa or Brenneria salicis
Lena Pesenti, Salomé Lengrand, Alexandra Kahn, Louis Michot, François Marchandise, Cyril Focant, Sophie Richet, Frédéric Debode, Claude Bragard
New Phytologist.2026; 251(1): 455. CrossRef
Role of Bacterial Endophytes in Bioremediation, Crop Improvement and Stress Management
Rajosik Bose, Harsh Dubey, Ayan Samadder, Smarto Basak, Sreya Dutta, Ranjit Kumar Dey, Amit Kumar Dixit, Shiddamallayya Mathapati, Sanjaya Kumar Y.R, Gajji Babu, Narayanam Srikanth, Rabinarayan Acharya, Susmita Roy
Journal of Crop Health.2025;[Epub] CrossRef
Transcriptome and Secretome Analyses of Endophyte Methylobacterium mesophilicum and Pathogen Xylella fastidiosa Interacting Show Nutrient Competition
Manuella Nobrega Dourado, Paulo Marques Pierry, Oseias Rodrigues Feitosa-Junior, Guillermo Uceda-Campos, Deibs Barbosa, Paulo A. Zaini, Abhaya M. Dandekar, Aline Maria da Silva, Welington Luiz Araújo
Microorganisms.2023; 11(11): 2755. CrossRef
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Nehaya Al-Karablieh, Ibtihal AbuObeid, Lina Al-Elaumi, Ihsan Mutlaq, Jihad Haddadin, Ruba Al Omari, Abdel-Munem Al-Jabaree, Safa Mazahreh
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Shahzad Munir, Ayesha Ahmed, Yongmei Li, Pengbo He, Brajesh K. Singh, Pengfei He, Xingyu Li, Suhail Asad, Yixin Wu, Yueqiu He
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Agnieszka Kaczmarczyk, Halina Kucharczyk, Marek Kucharczyk, Przemysław Kapusta, Jerzy Sell, Sylwia Zielińska
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Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria
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Sebastiàn Lòpez-Fernàndez, Valerio Mazzoni, Federico Pedrazzoli, Ilaria Pertot, Andrea Campisano
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Francisco M. Cazorla, Jesús Mercado-Blanco
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A stable Leifsonia xyli subsp. xyli GFP‐tagged strain reveals a new colonization niche in sugarcane tissues
M. C. Quecine, T. M. Silva, G. Carvalho, S. Saito, M. Mondin, N. S. Teixeira‐Silva, L. E. A. Camargo, C. B. Monteiro‐Vitorello
Plant Pathology.2016; 65(1): 154. CrossRef
Effects of growth-promoting endophytic Methylobacterium on development of Citrus rootstocks
Cristina Bogas Andr eacute a, Ivan Aguilar Vildoso Carlos, Aparecida Camargo Neves Aline, Luiz Ara uacute jo Welington
African Journal of Microbiology Research.2016; 10(19): 646. CrossRef
The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants
João Lúcio Azevedo, Welington Luiz Araújo, Paulo Teixeira Lacava
Genetics and Molecular Biology.2016; 39(4): 476. CrossRef
Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented MethylotrophicMethylobacteriumspp.
Manuella Nóbrega Dourado, Aline Aparecida Camargo Neves, Daiene Souza Santos, Welington Luiz Araújo
BioMed Research International.2015; 2015: 1. CrossRef
Differential gene expression in Xylella fastidiosa 9a5c during co‐cultivation with the endophytic bacterium Methylobacterium mesophilicum SR1.6/6
Manuella Nóbrega Dourado, Daiene Souza Santos, Luiz Roberto Nunes, Regina Lúcia Batista da Costa de Oliveira, Marcus Vinicius de Oliveira, Welington Luiz Araújo
Journal of Basic Microbiology.2015; 55(12): 1357. CrossRef
Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction
Welington Luiz Araújo, Daiene Souza Santos, Francisco Dini-Andreote, Jennifer Katherine Salgueiro-Londoño, Aline Aparecida Camargo-Neves, Fernando Dini Andreote, Manuella Nóbrega Dourado
Antonie van Leeuwenhoek.2015; 108(4): 951. CrossRef
Salt tolerant Methylobacterium mesophilicum showed viable colonization abilities in the plant rhizosphere
Dilfuza Egamberdieva, Stephan Wirth, Abdulaziz A. Alqarawi, E.F. Abd_Allah
Saudi Journal of Biological Sciences.2015; 22(5): 585. CrossRef
Endophytic microorganisms—promising applications in bioremediation of greenhouse gases
Z. Stępniewska, A. Kuźniar
Applied Microbiology and Biotechnology.2013; 97(22): 9589. CrossRef
A 454 Survey Reveals the Community Composition and Core Microbiome of the Common Bed Bug (Cimex lectularius) across an Urban Landscape
Matthew Meriweather, Sara Matthews, Rita Rio, Regina S. Baucom, Colin Dale
PLoS ONE.2013; 8(4): e61465. CrossRef
Draft Genome Sequence of Methylobacterium mesophilicum Strain SR1.6/6, Isolated from Citrus sinensis
Diogo Marinho Almeida, Francisco Dini-Andreote, Aline Aparecida Camargo Neves, Rommel Thiago Jucá Ramos, Fernando Dini Andreote, Adriana Ribeiro Carneiro, André Oliveira de Souza Lima, Pablo Henrique Caracciolo Gomes de Sá, Maria Silvanira Ribeiro Barbosa
Genome Announcements.2013;[Epub] CrossRef
Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa
Antônio Sérgio Ferreira Filho, Maria Carolina Quecine, Andréa Cristina Bogas, Priscilla de Barros Rossetto, Andre Oliveira de Souza Lima, Paulo Teixeira Lacava, João Lúcio Azevedo, Welington Luiz Araújo
World Journal of Microbiology and Biotechnology.2012; 28(4): 1475. CrossRef
Conservation and Diversity of Seed Associated Endophytes in Zea across Boundaries of Evolution, Ethnography and Ecology
David Johnston-Monje, Manish N. Raizada, M. Thomas P. Gilbert
PLoS ONE.2011; 6(6): e20396. CrossRef

Diversity of Endophytic Enterobacteria Associated with Different Host Plants: Adalgisa Ribeiro Torres , Welington Luiz Araujo , Luciana Cursino , Mariangela Hungria , Fabio Plotegher , Fabio Luis Mostasso , Joao Lucio Azevedo; J. Microbiol. 2008;46(4):373-379. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-007-0165-9

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Abstract PDF

Fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. Susceptibility was found on both tested antibiotics. However, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. Cellulase production also changed according to host plants. The diversity of these isolates was estimated by employing BOX-PCR genomic fingerprints and 16S rDNA sequencing. In total, twenty-three distinct operational taxonomic units (OTUs) were identified by employing a criterion of 60% fingerprint similarity as a surrogate for an OTU. The 23 OTUs belong to the Pantoea and Enterobacter genera, while their high diversity could be an indication of paraphyletic classification. Isolates representing nine different OTUs belong to Pantoea agglomerans, P. ananatis, P. stewartii, Enterobacter sp., and E. homaechei. The results of this study suggest that plant species may select endophytic bacterial genotypes. It has also become apparent that a review of the Pantoea/Enterobacter genera may be necessary.

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The Endophyte Curtobacterium flaccumfaciens Reduces Symptoms Caused by Xylella fastidiosa in Catharanthus roseus: Paulo Teixeira Lacava , Wenbin Li , Welington Luiz Araujo , Joao Lucio Azevedo , John Stephen Hartung; J. Microbiol. 2007;45(5):388-393.; DOI: https://doi.org/2599 [pii]

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Abstract PDF: Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.

Evaluation of Endophytic Colonization of Citrus sinensis and Catharanthus roseus Seedlings by Endophytic Bacteria: Paulo Teixeira Lacava , Welington Luiz Araujo , Joao Lucio Azevedo; J. Microbiol. 2007;45(1):11-14.; DOI: https://doi.org/2498 [pii]

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Abstract PDF: Over the last few years, the endophytic bacterial community associated with citrus has been studied as an important component interacting with Xylella fastidiosa, the causal agent of citrus variegated chlorosis (CVC). This bacterium may also colonize some model plants, such as Catharanthus roseus and Nicotiana clevelandii. In the present study, we compared the endophytic colonization of Citrus sinensis and Catharanthus roseus using the endophytic bacteria Klebsiella pneumoniae. We chose an appropriate strain, K. pneumoniae 342 (Kp342), labeled with the GFP gene. This strain was inoculated onto seedlings of C. sinensis and C. roseus. The isolation frequency was determined one week after the inoculation and the endophytic colonization of K. pneumoniae was observed using fluorescence microscopy. Although the endophytic bacterium was more frequently isolated from C. roseus than from C. sinensis, the colonization profiles for both host plants were similar, suggesting that C. roseus could be used as a model plant to study the interaction between endophytic bacteria and X. fastidiosa.

A Bacterium Belonging to the Burkholderia cepacia Complex Associated with Pleurotus ostreatus: Ricardo Yara , Walter Maccheroni Junior , Jorge Horii , Joao Lucio Azevedo; J. Microbiol. 2006;44(3):263-268.; DOI: https://doi.org/2387 [pii]

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Abstract PDF: Pleurotus ostreatus is a widely cultivated white-rot fungus. Owing to its considerable enzymatic versatility P. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. Interactions between microorganisms can be an important factor in those processes. In this study, we describe the presence of a bacterial species associated with P. ostreatus strain G2. This bacterial species grew slowly (approximately 30 days) in theliquid and semi-solid media tested. When P. ostreatus was inoculated in solid media containing Tween 80 or Tween 20, bacterial microcolonies were detected proximal to the fungal colonies, and the relevant bacterium was identified via the analysis of a partial 16S rDNA sequence; it was determined to belong to the Burkholderia cepacia complex, but was not closely related to other fungus-isolated Burkholderiaceae. New specific primers were designed, and confirmed the presence of in vitro P. ostreatus cultures. This is the first time that a bacterial species belonging to the B. cepacia complex has been found associated with P. ostreatus.

Impact of Genetically Modified Enterobacter cloacae on Indigenous Endophytic Community of Citrus sinensis Seedlings: Fernando Dini Andreote , Marcelo Jose Mortatti Gullo , Andre Oliveira de Souza Lima , Walter Maccheroni Junior , Joao Lucio Azevedo , Welington Luiz Araujo; J. Microbiol. 2004;42(3):169-173.

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Abstract PDF: Enterobacter cloacae (strain PR2/7), a genetically modified endophyte (GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.

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