- Morphologies and phenotypes in Bacillus subtilis biofilms
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Xiaoling Wang , Shuo Meng , Jingshi Han
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J. Microbiol. 2017;55(8):619-627. Published online July 4, 2017
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DOI: https://doi.org/10.1007/s12275-017-7041-z
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In this study, we explored Bacillus subtilis biofilm growth
under various conditions such as the use of substrates with
different stiffnesses and nutrient levels using a well-developed
optical imaging technique to spatially and temporally
track biofilm growth. We also developed a quantitative method
to characterize B. subtilis biofilm morphologies under
various growth conditions. To determine biofilm rim irregularities,
we used the dimensionless P2A ratio, defined as
P2/4πA, where P is the perimeter and A is the area of the
biofilm. To estimate biofilm thickness from transmission
images, we developed a calibration procedure based on Beer-
Lambert’s law and cross sectioning. Furthermore, to determine
the distributions of different B. subtilis cell phenotypes
during biofilm growth, we used a triple-fluorescence-labeled
B. subtilis strain that expressed motility, matrix production,
and sporulation. Based on this work, we are able to tune biofilm
growth by changing its growing environment.
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Citations
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