- Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay
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Jing Ping Zhu , Xian Li , Shi Du
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J. Microbiol. 2012;50(2):186-190. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1418-9
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Based on sequence analyses of phycocyanin intergenic spacers
(PC-IGS) from Microcystis, Anabaena, Aphanizomenon,
and Planktothrix (Oscillatoria) strains, a genus-specific probe
pair TF/TR was designed, and a sandwich hybridization assay
was established to quantitatively detect Microcystis. Through
BLAST and cyanobacterial culture tests, TF/TR was demonstrated
to be specific for Microcystis. A calibration curve
for the sandwich hybridization assay was established, and
the lowest detected concentration was 100 cell/ml. Laboratory
and field samples were analyzed with both sandwich hybridization
assay and microscopy. The biotic and abiotic
components of the samples were of little disturbance to the
sandwich hybridization assay. The results showed no distinct
difference between the two methods. In this study, a sandwich
hybridization assay was established to detect Microcystis,
providing an alternative to traditional microscopic, morphology-
based methods.
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Citations
Citations to this article as recorded by  - The Need to Increase Strain-Specific DNA Information from the Invasive Cyanobacteria Sphaerospermopsis aphanizomenoides and Cuspidothrix issatschenkoi
Daniela R. de Figueiredo
Water.2025; 17(4): 579. CrossRef - Molecular techniques for understanding harmful algal blooms: A review
Jackson Sanders, Senjie Lin
Harmful Algae.2025; 148: 102909. CrossRef - Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review
Ping Gong, Anna K. Antrim, Sarah R. Bickman, Emily G. Cooley, Seung Ho Chung
Biosensors.2022; 12(8): 640. CrossRef
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