Journal of Microbiology

Jing Li

2 Articles

Extracellular products-mediated interspecific interaction between Pseudomonas aeruginosa and Escherichia coli: Yang Yuan , Jing Li , Jiafu Lin , Wenjuan Pan , Yiwen Chu , Balakrishnan Prithiviraj , Yidong Guo , Xinrong Wang , Kelei Zhao; J. Microbiol. 2021;59(1):29-40. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0478-0

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The Gram-negative pathogen Pseudomonas aeruginosa adopts several elaborate strategies to colonize a wide range of natural or clinical niches and to overcome the neighboring bacterial competitors in polymicrobial communities. However, the relationship and interaction mechanism of P. aeruginosa with other bacterial pathogens remains largely unexplored. Here we explore the interaction dynamics of P. aeruginosa and Escherichia coli, which frequently coinfect the lungs of immunocompromised hosts, by using a series of on-plate proximity assays and RNA-sequencing. We show that the extracellular products of P. aeruginosa can inhibit the growth of neighboring E. coli and induce a large-scale of transcriptional reprogramming of E. coli, especially in terms of cellular respiration- related primary metabolisms and membrane components. In contrast, the presence of E. coli has no significant effect on the growth of P. aeruginosa in short-term culture, but causes a dysregulated expression of genes positively controlled by the quorum-sensing (QS) system of P. aeruginosa during subsequent pairwise culture. We further demonstrate that the divergent QS-regulation of P. aeruginosa may be related to the function of the transcriptional regulator PqsR, which can be enhanced by E. coli culture supernatant to increase the pyocyanin production by P. aeruginosa in the absence of the central las-QS system. Moreover, the extracellular products of E. coli promote the proliferation and lethality of P. aeruginosa in infecting the Caenorhabditis elegans model. The current study provides a general characterization of the extracellular products-mediated interactions between P. aeruginosa and E. coli, and may facilitate the understanding of polymicrobial infections.

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SERS-based approaches in the investigation of bacterial metabolism, antibiotic resistance, and species identification
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Impact of Clostridium butyricum HADIG-CB003 dietary supplementation on the gut microbiota of Kunming mice
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A fluorescence-based protocol to monitor bacterial antagonism
Justin M. Luu, Cristian V. Crisan, Morgan L. Pettis, Anayancy Ramos Facio, Timothy D. Read, Joanna B. Goldberg, Justin R. Kaspar
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Rapid culture-free species identification and phenotypic antibiotic susceptibility test for polymicrobial infection by single-cell Raman spectroscopy
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Pigments from pathogenic bacteria: a comprehensive update on recent advances
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Selective detection of two bacterial species in a single collision system targeting metabolic products
Jun Lin, Qingwen Wang, Huike Tian, Qing Xin, Dong Zhang
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Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
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Safety Evaluation In Vitro of Enterococcus durans from Tibetan Traditional Fermented Yak Milk: Jing Li , Fazheng Ren , Huiyong Gu , Xiaopeng Li , Bozhong Gan; J. Microbiol. 2011;49(5):721-728. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1062-9

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Despite its ubiquity in fermented dairy products, the safety of lactic acid enterococcal bacteria remains controversial. In this study, five Enterococcus durans strains – A1, A2, B1, B2, and C1 – were isolated from traditional fermented yak milk from Tibet. To evaluate the strains’ safety, biogenic amine production, antibiotic resistance and presence of known virulence determinants were investigated. Strain A1 can produce biogenic amines for histamine, spermine, and spermidine (mean values: 8.64, 8.31, and 0.30 mg/L, respectively). Polymerase chain reaction amplification for Strain A1 found genes involved in expression of gelatinase (gleE), cytolysin (cylA, cylB, and cylM), sex pheromones (ccf and cpd) and cell wall adhesion (efaA). Strain A2 showed sensitivity or intermediate resistance to all tested antibiotics, and no virulence determinants except gelE and ccf, but did produce tyramine at a relatively high level (912.02 mg/L). Both strains B1 and B2 could produce histamine (10.43 and 10.56 mg/L, respectively), and showed vancomycin resistance; B1 also produced tyramine (504.02 mg/L). Strain C1 could produce all five biogenic amines tested in the study – putrescine, histamine, tyramine, spermine, and spermidine; concentrations were 6.51, 9.59, 205.85, 5.55, and 5.39 mg/L, respectively. All E. durans strains found in Tibetan traditional fermented yak milk thus offer potential risk.

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Biological Characteristics and Safety Examination of Five Enterococcal Strains from Probiotic Products
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Safety evaluation of Lactobacillus paracasei subsp. paracasei LC-01, a probiotic bacterium
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Journal of Microbiology.2013; 51(5): 633. CrossRef

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