- Cytokine-Inducing and T Cell Mitogenic Effects of Cordyceps hepialidicola
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Jong-Soon Lim , Seung-Hyung Kim , Jeong-Youl Choi , Jin-Seo Park , Seong Joo Park , Kwang-Soo Shin
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J. Microbiol. 2001;39(3):181-185.
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The morphological characteristics of newly isolated Cordyceps hepialidicola were characterized, and the phylogenetic relationships with other Cordyceps species were investigated using a sequence analysis of the internal transcribed spacer (ITS). The PCR product of 592 bp showed a homology of 92 and 91% with C. militaris and C. nutans, respectively. In an in vitro model using mouse peripheral blood mononuclear cells (PBMC), a methanol extract of C. hepialidicola induced multiple cytokines, including IFN-[gamma], IL-4, and IL-18. The extract also enhanced the percentages of the CD4^+ and CD8^+ T cells in the healthy murine PBMCs to 56.1% and 13.0%, respectively. The percentages of CD4^+ and CD8^+ in the untreated controls were 28.4 and 7.3%, and concanavalin A-treated positive controls were 62.4 and 18.3%, respectively.
- Anti-tumor Activity of the Fruitbody Extract of Basidiomycete, Phellinus linteus
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Jong-Soon Lim , Seung-Hyung Kim , Jin-Seo Park , Jeong-Youl Choi , Seong Joo Park , Kwang-Soo Shin
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J. Microbiol. 2001;39(2):121-125.
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Methanol extract prepared from the fruitbody of Phellinus linteus (EPL) showed anti-tumor and immuno-stimulating activities. The invasion activity of B16-F10 melanoma cells through a reconstituted basement membrane to the collagen-coated lower surface of the filters was inhibited about 67% by EPL (100 ug/ml). Also, EPL inhibited the expression of the mRNA for MMP-2 and MMP-9. In vivo treatment of C57BL/6 mice (150 mg/kg) with EPL for 14 days, the pulmonary colonization was found to be inhibited about 75%. Using reverse transcriptionpolymerase chain reaction (RT-PCR) analysis, we found that cytokine IL-12 and INF-[gamma] genes were induced by EPL. Furthermore, EPL stimulated the proliferation of CD4^+ (33.5%) and CD8^+ (17.7%) in mouse splenocytes.
- Isolation of a Medium Chain Length Polyhydroxyalkanoic Acids Degrading Bacterium, Janthinobacterium lividum
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Jin-Seo Park , Jeong-Youl Choi , Pil-Mun Joung , Seong Joo Park , Young Ha Rhe e , Kwang-Soo Shin
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J. Microbiol. 2001;39(2):139-141.
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Medium-chain length polyhydroxyalkanoic acids (MCL-PHAs) degrading bacterium was isolated from the soil. The bacterium was identified as Janthinobacterium lividum by its biochemical properties, cell membrane fatty acids composition, and 16S rDNA sequence analysis. The bacterium showed a similarity of 0.911 with J. lividum according to the cell membrane fatty acids analysis and a similarity of 97% in the 16S rDNA sequence analysis. Culture supernatant of the bacterium showed the highest depolymerase activity toward polyhydroxynonanoic acid (PHN) that did not degrade the poly-[beta]-hydroxybutyric acid (PHB). The esterase activity was also detected with p-nitrophenyl (PNP) esters of fatty acids such as PNP-octanoic acid, PNP-dodecanoic acid, PNP-decanoic acid, and PNP-hexanoic acid.
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