- Characterization, Cloning, and Heterologous Expression of a Subtilisin-Like Serine Protease Gene VlPr1 from Verticillium lecanii
-
Gang Yu , Jin-Liang Liu , Li-Qin Xie , Xue-Liang Wang , Shi-Hong Zhang , Hong-Yu Pan
-
J. Microbiol. 2012;50(6):939-946. Published online December 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2199-x
-
-
694
View
-
0
Download
-
13
Crossref
-
 Abstract
 PDF
-
The entomopathogenic fungus Verticillium lecanii is a wellknown biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease gene VlPr1 was cloned from the fungus and the VlPr1 protein was expressed in Escherichia coli. The VlPr1 gene contains
an open reading frame (ORF) interrupted by three short introns, and encodes a protein of 379 amino acids. Protein sequence analysis revealed high homology with subtilisin serine proteases. The molecular mass of the protease was 38 kDa, and the serine protease exhibited its maximal activity at 40°C and pH 9.0. Protease activity was also affected by Mg2+ and Ca2+ concentration. The protease showed inhibitory activity against several plant pathogens, especially towards Fusarium moniliforme.
-
Citations
Citations to this article as recorded by  - Blocking miR439 enhances rice disease resistance and yield
Yong Zhu, Ji-Fen Sun, Xin-Hui Zhou, Xue-Mei Yang, Si-Yao Zhang, Yu-Kun Peng, Yu Liu, Hao Su, Xiao-Yu Xiong, Yuan Yang, Ling-Li Zhang, De-Qiang Li, He Wang, Guo-Bang Li, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Plant Physiology.2026;[Epub] CrossRef - Verticillium dahliae Elicitor VdSP8 Enhances Disease Resistance Through Increasing Lignin Biosynthesis in Cotton
Yajuan Wang, Jun Qin, Mengmeng Wei, Xiwen Liao, Wenjing Shang, Jieyin Chen, Krishna V. Subbarao, Xiaoping Hu
Plant, Cell & Environment.2025; 48(1): 728. CrossRef - Interaction of secreted protein PgSLP and citrus calmodulin-like protein CsCML27/44 in fruit disease defense
Yao Xu, Ou Chen, Rong Zhu, Rui Huang, Jian Ming, Shixiang Yao, Kaifang Zeng
Postharvest Biology and Technology.2025; 230: 113757. CrossRef - Subtilisin-like proteases from Fusarium graminearum induce plant cell death and contribute to virulence
Jiang Xiong, Mingyu Luo, Yunshen Chen, Qianyong Hu, Ying Fang, Tongjun Sun, Guanjing Hu, Cui-Jun Zhang
Plant Physiology.2024; 195(2): 1681. CrossRef - The fungal protease BbAorsin contributes to growth, conidiation, germination, virulence, and antiphytopathogenic activities in Beauveria bassiana (Hypocreales: Cordycipitaceae)
Bang-Xian Zhang, Fang-Fang Liu, Feng Liu, Wen-Xuan Qi, Yan-Qin Si, Hai-Yan Ren, Cheng-Qi Zhang, Xiang-Jun Rao
Pesticide Biochemistry and Physiology.2024; 202: 105936. CrossRef - Dual biocontrol potential of the entomopathogenic fungus Akanthomyces muscarius against Thaumetopoea pityocampa and plant pathogenic fungi
Amal Saidi, Samira Mebdoua, Dalila Mecelem, Nawal Al-Hoshani, Nouari Sadrati, Fehmi Boufahja, Hamdi Bendif
Saudi Journal of Biological Sciences.2023; 30(8): 103719. CrossRef - A secreted fungal subtilase interferes with rice immunity via degradation of SUPPRESSOR OF G2 ALLELE OF skp1
Xiaoyang Chen, Xiabing Li, Yuhang Duan, Zhangxin Pei, Hao Liu, Weixiao Yin, Junbin Huang, Chaoxi Luo, Xiaolin Chen, Guotian Li, Kabin Xie, Tom Hsiang, Lu Zheng
Plant Physiology.2022; 190(2): 1474. CrossRef - Entomogenous fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity
Xiaoqiu Cui, Huiyun Wang, Hao Yang, Xiao Li, Xiuzhen Li
Antonie van Leeuwenhoek.2020; 113(10): 1507. CrossRef - Network analysis exposes core functions in major lifestyles of fungal and oomycete plant pathogens
Eswari PJ Pandaranayaka, Omer Frenkel, Yigal Elad, Dov Prusky, Arye Harel
BMC Genomics.2019;[Epub] CrossRef - An approach to select Lactobacillus isolates as protective cultures for food fermentations
Raffael C. Inglin, Alessia I. Delbrück, Benjamin Fässler, Katharina E. Siebenmann, Christophe Lacroix, Marc J. A. Stevens, Leo Meile
Journal of Food Safety.2018;[Epub] CrossRef - Subtilisin‐like proteases in plant defence: the past, the present and beyond
Joana Figueiredo, Marta Sousa Silva, Andreia Figueiredo
Molecular Plant Pathology.2018; 19(4): 1017. CrossRef - Phylogenetic analyses reveal molecular signatures associated with functional divergence among Subtilisin like Serine Proteases are linked to lifestyle transitions in Hypocreales
Deepti Varshney, Akanksha Jaiswar, Alok Adholeya, Pushplata Prasad
BMC Evolutionary Biology.2016;[Epub] CrossRef - Enzymes of Entomopathogenic Fungi, Advances and Insights
Lluvia de Carolina Sánchez-Pérez, Juan Esteban Barranco-Florido, Silvia Rodríguez-Navarro, José Francisco Cervantes-Mayagoitia, Miguel Ángel Ramos-López
Advances in Enzyme Research.2014; 02(02): 65. CrossRef
|
MSK
