- Nup211, the Fission Yeast Homolog of Mlp1/Tpr, Is Involved in mRNA Export
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Jin-Ah Bae , DongGeRaMi Moon , Jin Ho Yoon
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J. Microbiol. 2009;47(3):337-343. Published online June 26, 2009
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DOI: https://doi.org/10.1007/s12275-009-0125-7
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Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlp1/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.
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Citations
Citations to this article as recorded by  - Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis
Domenick Kamel, Ayisha Sookdeo, Ayana Ikenouchi, Hualin Zhong, Juan Mata
PLOS ONE.2024; 19(12): e0312095. CrossRef - Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe
Joseph M Varberg, Jay R Unruh, Andrew J Bestul, Azqa A Khan, Sue L Jaspersen
Life Science Alliance.2022; 5(7): e202201423. CrossRef - Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast
Haruhiko Asakawa, Tomoko Kojidani, Hui-Ju Yang, Chizuru Ohtsuki, Hiroko Osakada, Atsushi Matsuda, Masaaki Iwamoto, Yuji Chikashige, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi, Gregory P. Copenhaver
PLOS Genetics.2019; 15(6): e1008061. CrossRef - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components
Silvia Salas-Pino, Paola Gallardo, Ramón R. Barrales, Sigurd Braun, Rafael R. Daga
Journal of Cell Biology.2017; 216(11): 3591. CrossRef - Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis
Dan Zhang, Snezhana Oliferenko, Daniel J. Lew
Molecular Biology of the Cell.2014; 25(19): 2970. CrossRef - Characterization of nuclear pore complex components in fission yeastSchizosaccharomyces pombe
Haruhiko Asakawa, Hui-Ju Yang, Takaharu G Yamamoto, Chizuru Ohtsuki, Yuji Chikashige, Kumiko Sakata-Sogawa, Makio Tokunaga, Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
Nucleus.2014; 5(2): 149. CrossRef - Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef - Double duty for nuclear proteins – the price of more open forms of mitosis
Colin P. De Souza, Stephen A. Osmani
Trends in Genetics.2009; 25(12): 545. CrossRef
- The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export
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DongGeRaMi Moon , Jin-Ah Bae , Hyun Jin Cho , Jin Ho Yoon
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J. Microbiol. 2008;46(4):422-428. Published online August 31, 2008
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DOI: https://doi.org/10.1007/s12275-008-0177-0
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We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.
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Citations
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Abigail Obuobi, Neils B. Quashie, Nancy Odurowah Duah-Quashie, Jon R. Sayers, Yash Gupta
PLOS One.2025; 20(12): e0338411. CrossRef - Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef - Control of mRNA Export and Translation Termination by Inositol Hexakisphosphate Requires Specific Interaction with Gle1
Abel R. Alcázar-Román, Timothy A. Bolger, Susan R. Wente
Journal of Biological Chemistry.2010; 285(22): 16683. CrossRef - Generation of expression vectors for high-throughput functional analysis of target genes in Schizosaccharomyces pombe
Jiwon Ahn, Chung-Hae Choi, Chang-Mo Kang, Chun-Ho Kim, Hee-Moon Park, Kyung-Bin Song, Kwang-Lae Hoe, Misun Won, Kyung-Sook Chung
The Journal of Microbiology.2009; 47(6): 789. CrossRef
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