Journal of Microbiology

Jin Hwan Park

2 Articles

Low Cell Density Regulator AphA Upregulates the Expression of Vibrio vulnificus iscR Gene Encoding the Fe-S Cluster Regulator IscR: Jong Gyu Lim , Jin Hwan Park , Sang Ho Choi; J. Microbiol. 2014;52(5):413-421. Published online February 17, 2014; DOI: https://doi.org/10.1007/s12275-014-3592-4

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IscR is a global transcriptional regulator that contributes to the pathogenesis of Vibrio vulnificus, a food-borne pathogen. In the present study, the regulatory mechanism for the iscR expression of V. vulnificus was evaluated. The expression of iscR was found to be upregulated by a transcriptional regu-lator AphA, a homologue of the low cell density regulator AphA of the Vibrio species, in the exponential phase of growth. The promoter activity of iscR appeared to be acti-vated and repressed by AphA and IscR, respectively. EMSA and DNase I protection assay showed that both AphA and IscR bind to the iscR regulatory region and the binding site for AphA overlapped with part of the binding site for IscR. Further mutational analysis suggested that AphA upregu-lates the iscR expression only in the presence of functional IscR. An examination of the roles of AphA and the binding sites revealed that the binding of AphA would hinder the IscR-mediated repression of the iscR transcription. The com-bined results show that V. vulnificus AphA upregulates iscR expression by antagonizing its negative autoregulation.

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Erin L. Mettert, Patricia J. Kiley
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A Nitric Oxide-Responsive Transcriptional Regulator NsrR Cooperates With Lrp and CRP to Tightly Control the hmpA Gene in Vibrio vulnificus
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The transcriptional regulator IscR integrates host-derived nitrosative stress and iron starvation in activation of the vvhBA operon in Vibrio vulnificus
Garam Choi, Kyung Ku Jang, Jong Gyu Lim, Zee-Won Lee, Hanhyeok Im, Sang Ho Choi
Journal of Biological Chemistry.2020; 295(16): 5350. CrossRef
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Renfei Lu, George Osei-Adjei, Xinxiang Huang, Yiquan Zhang
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What a difference a cluster makes: The multifaceted roles of IscR in gene regulation and DNA recognition
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Characterization of the Vibrio vulnificus 1-Cys Peroxiredoxin Prx3 and Regulation of Its Expression by the Fe-S Cluster Regulator IscR in Response to Oxidative Stress and Iron Starvation
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Journal of Biological Chemistry.2014; 289(52): 36263. CrossRef

Effects of Nutritional Enrichment on the Production of Acetone-Butanol-Ethanol (ABE) by Clostridium acetobutylicum: Sung Jun Choi , Joungmin Lee , Yu-Sin Jang , Jin Hwan Park , Sang Yup Lee , In Ho Kim; J. Microbiol. 2012;50(6):1063-1066. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2373-1

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Abstract PDF

Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. acetobutylicum medium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19.7, which is 1.8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0.83 g/L/h, which is 1.5 times higher than that (0.55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.

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