Journal of Microbiology

Jin Hee Han

1 Article

Use of the Yeast 1.5-Hybrid System to Detect DNA-Protein-Protein Interactions: Sook-Kyung Kim , Jin Hee Han; J. Microbiol. 2000;38(2):113-116.

Abstract PDF: Escherichia coli F plasmid partition apparatus is composed of two trans-acting proteins (SopA and SopB) and one cis-acting DNA sequence (sopC). The SopB-sopC complex has been suggested to serve a centromere-like function through its interaction with chromosomally encoded proteins which remain to be identified. In this paper, we are introducing a new yeast 1.5-hybrid system which assembles the two-hybrid and one-hybrid system as a mean to find an additional component of the F plasmid partition system, interacting with DNA (sopC)-bound SopB protein. The result indicates that this system is a promising one, capable of selecting an interacting component.

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