- In vitro disinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio isolates in Pacific white shrimp Penaeus vannamei
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Peizhuo Zou , Qian Yang , Hailiang Wang , Guosi Xie , Zhi Cao , Xing Chen , Wen Gao , Jie Huang
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J. Microbiol. 2020;58(8):675-686. Published online July 27, 2020
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DOI: https://doi.org/10.1007/s12275-020-9537-1
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Acute hepatopancreatic necrosis disease (AHPND) is one
of the most significant bacterial diseases in global shrimp
culture, causing severe economic losses. In the present study,
we carried out in vitro antimicrobial tests to investigate the
disinfection efficacy of 14 common disinfectants toward different
AHPND-causing Vibrio spp., including eight isolates
of V. parahaemolyticus, four isolates of V. campbellii, and
one isolate of V. owensii. Polyhexamethylene biguanidine hydrochloride
(PHMB) was revealed to possess the strongest
inhibitory activity. Through analyzing and evaluating the results
of antimicrobial tests and acute toxicity test, we selected
PHMB and hydrogen peroxide (H2O2) for further clinical
protection test. Clinical manifestations indicated that both
PHMB (2 mg/L and 4 mg/L) and H2O2 (12 mg/L) could effectively
protect juvenile Penaeus vannamei from the infection
of V. parahaemolyticus isolate Vp362 at 106 CFU/ml, and the
survival rate was over 80%. When the bacterial concentration
was reduced to 105 CFU/ml, 104 CFU/ml, and 103 CFU/ml,
the survival rate after treated by 1 mg/L PHMB was 64.44%,
93.33%, and 100%, respectively. According to the results,
PHMB and H2O2 showed a lower toxicity while a better protection
activity, particularly against a lower concentration of
the pathogens. Therefore, these two disinfectants are proved
to be promising disinfectants that can be applied to prevent
and control AHPND in shrimp culture. Moreover, the methods
of this study also provided valuable information for the
prevention of other important bacterial diseases and suggested
a reliable means for screening potential drugs in aquaculture.
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Citations
Citations to this article as recorded by  - Comparative In vitro Evaluation of Widely Used Aquaculture Disinfectants Against Major Marine Bacterial Fish Pathogens
T. G. Sumithra, S. Aparna, Andria, N. S. Ashily, V. N. Anusree, P. K. Patil, S. R. Krupesha Sharma
Journal of Fish Diseases.2026;[Epub] CrossRef - Bactericidal Effect and Mechanism of Polyhexamethylene Biguanide (PHMB) on Pathogenic Bacteria in Marine Aquaculture
Lanting Wu, Chunyuan Wang, Yingeng Wang, Yongxiang Yu, Zheng Zhang, Cuiping Ma, Xiaojun Rong, Ling Chen, Meijie Liao, Yapeng Yang
Biology.2025; 14(5): 470. CrossRef - Comparative disinfection efficacy in aquaculture: novel methylene bis(thiocyanate) vs. conventional trichloroisocyanuric acid against Aeromonas hydrophila
Guang Yang, Ying Huang, Ning Ma, Kai Li, Xiao-mei Wang, Lian-bo Zhang, Wen-bo Yang, Wan-li Zhang, Lei Xia, Hong-Yu Zhang, Li-lai Yuan
Frontiers in Microbiology.2025;[Epub] CrossRef - Basigin binds bacteria and activates Dorsal signaling to promote antibacterial defense in Penaeus vannamei
Linwei Yang, Zi-ang Wang, Yushi Gan, Hongliang Zuo, Hengwei Deng, Shaoping Weng, Jianguo He, Xiaopeng Xu
Fish & Shellfish Immunology.2023; 142: 109123. CrossRef - Clinical protective effects of polyhexamethylene biguanide hydrochloride (PHMB) against Vibrio parahaemolyticus causing translucent post-larvae disease (VTPD) in Penaeus vannamei
Tianchang Jia, Tingting Xu, Jitao Xia, Shuang Liu, Wenqiang Li, Ruidong Xu, Jie Kong, Qingli Zhang
Journal of Invertebrate Pathology.2023; 201: 108002. CrossRef
- Diluted conventional media improve the microbial cultivability from aquarium seawater
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Jing Sun , Jiazhen Guo , Qian Yang , Jie Huang
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J. Microbiol. 2019;57(9):759-768. Published online August 3, 2019
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DOI: https://doi.org/10.1007/s12275-019-9175-7
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1,066
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Abstract
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The cultivation of microbial species remains a primary challenge
in microbiology and obtaining pure cultures is essential
for the study of microbial physiology and function. When
isolating microorganisms from aquaculture environments,
Vibrio are the most dominate isolates on the media that are
commonly used. In order to expand our ability to study microbial
species, an easy-operation and low-cost medium that
can reduce the interference of Vibrio strains and increase the
cultivability of other bacteria is urgently needed. We compared
viable cell counts on conventional media (CM; including
Marine Agar 2216 and LB media) and diluted media (DM;
including 1/10-Marine Agar 2216, 1/10-LB). We also assessed
the diversity of cultivable microorganisms under high and
low nutrient conditions by a plate-wash strategy coupled with
high-throughput sequencing of the V4 hypervariable region
of the 16S rRNA gene. The results show that microbial communities
from DM, especially 1/10-Marine Agar 2216, are
more diverse than those obtained from CM. Vibrio isolates
were reduced on DM. PICRUSt analysis revealed that nutrient
composition is a significant contributor to the diversity
and function of the cultivable microbial communities. Bacteria
grown on CM possess more pathogenic characteristics,
whereas DM favors the growth of bacteria that have multiple
metabolic functions. Collectively, our data provide strong evidence
that dilution of CM influences the cultivability of bacteria
from aquaculture seawater. It also supports that DM can
expand the range of microbial species that can be cultivated.
This study also provides insights for media design in microbial
cultivation from aquaculture systems.
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Citations
Citations to this article as recorded by - Metagenomic insights into salinity-driven plasticity of the shrimp gut microbiome and its resistome
Xueqin Yang, Xiuli Chen, Min Peng, Yongzhen Zhao, Xia Wang
Aquaculture.2027; 626: 744449. CrossRef - In vitro domestication of halophyte microbiota for future SynCom application
Nada A. Moner, Tarek R. Elsayed, Wafaa M. Amer, Mervat A. Hamza, Hanan H. Youssef, Randa M. Abdel-Fatah, Mahmoud S. Abdelwahab, Omar M. Shahat, Gehan H. Youssef, Mahmoud El-Tahan, Mohamed Fayez, Florian Fricke, Silke Ruppel, Nabil A. Hegazi
Plant and Soil.2026; 523(2): 947. CrossRef - Use of metabarcoding detects the rapid onset of cultivation bias in the culture-based profiling of marine sediment bacterial communities
Seokwoo Jo, Hyun-Gwan Lee, Dong-Ha Nam, Chungoo Park
Letters in Applied Microbiology.2026;[Epub] CrossRef - Comparison of methods for the enumeration of heterotrophic bacteria in seawater from the Adriatic Sea: Implications for aquaculture
Damir Kapetanović, Arijana Cenov, Dražen Lušić, Brigita Novosel, Darija Vukić Lušić
Estuarine, Coastal and Shelf Science.2026; 336: 109825. CrossRef - Isolation and Identification of PHA-Producing Haloalkaliphilic Bacteria from Bledug Kesongo Mud Volcano
Anto Budiharjo, Isnanda Danur Nugroho, Wijanarka Wijanarka, Dyah Wulandari, Mukhammad Asy'ari
Biointerface Research in Applied Chemistry.2026;[Epub] CrossRef - Heterologous Expression and Functional Analysis of Exiguobacterium Algin Lyase Gene by Pichia pastoris
Hanwen Wu, Kai Hou, Yutong Jiang, Mingjian Luan, Yuxia Sun, Xi He, Xiangzhong Zhao
Fermentation.2025; 11(1): 34. CrossRef - Microbial diversity and ecological roles of halophilic microorganisms in Dingbian (Shaanxi, China) saline-alkali soils and salt lakes
Yue Ding, Juntao Ke, Tao Hong, Aodi Zhang, Xue Wu, Xinran Jiang, Shilong Shao, Ming Gong, Shengda Zhao, Liang Shen, Shaoxing Chen
BMC Microbiology.2025;[Epub] CrossRef - Unexpected methane oxidation acceleration by two species of bacteria from the rainwater promoting Methylomonas sp. in the soil
Yuxuan Sang, Jia Tang, Oumei Wang, Qinqin Hao, Shiling Zheng, Fanghua Liu
Journal of Environmental Chemical Engineering.2024; 12(5): 113895. CrossRef - Glucose addition improves the culture performance of Pacific white shrimp by regulating the assembly of Rhodobacteraceae taxa in gut bacterial community
Pengsheng Dong, Haipeng Guo, Lei Huang, Demin Zhang, Kai Wang
Aquaculture.2023; 567: 739254. CrossRef - Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons
Gabriela Kapinusova, Marco A. Lopez Marin, Ondrej Uhlik
Frontiers in Microbiology.2023;[Epub] CrossRef - The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth
Haifeng Wang, Juan Guo, Xing Chen, Hongxuan He
Metabolites.2023; 13(8): 958. CrossRef - Conjugative Transfer of Acute Hepatopancreatic Necrosis Disease-Causing pVA1-Type Plasmid Is Mediated by a Novel Self-Encoded Type IV Secretion System
Dehao Wang, Liying Wang, Dexi Bi, Jipeng Song, Guohao Wang, Ye Gao, Kathy F. J. Tang, Fanzeng Meng, Jingmei Xie, Fan Zhang, Jie Huang, Jianliang Li, Xuan Dong, Jennifer M. Auchtung, Songzhe Fu
Microbiology Spectrum.2022;[Epub] CrossRef - Culturomics of Bacteria from Radon-Saturated Water of the World’s Oldest Radium Mine
Gabriela Kapinusova, Kunal Jani, Tereza Smrhova, Petr Pajer, Irena Jarosova, Jachym Suman, Michal Strejcek, Ondrej Uhlik, Jing Han
Microbiology Spectrum.2022;[Epub] CrossRef - Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria
Kazuo Yamamoto, Shotaro Toya, Sarah Sabidi, Yuki Hoshiko, Toshinari Maeda
Applied Microbiology and Biotechnology.2021; 105(9): 3787. CrossRef - Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota
Hend Elsawey, Sascha Patz, Rahma A. Nemr, Mohamed S. Sarhan, Mervat A. Hamza, Hanan H. Youssef, Mohamed R. Abdelfadeel, Hassan-Sibroe A. Daanaa, Mahmoud El-Tahan, Mohamed Abbas, Mohamed Fayez, Katja Witzel, Silke Ruppel, Nabil A. Hegazi
Diversity.2020; 12(11): 418. CrossRef
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