- SP0454, A Putative Threonine Dehydratase, Is Required For Pneumococcal Virulence In Mice
-
WenJuan Yan , Hong Wang , WenChun Xu , KaiFeng Wu , Run Yao , XiuYu Xu , Jie Dong , YanQing Zhang , Wen Zhong , XueMei Zhang
-
J. Microbiol. 2012;50(3):511-517. Published online June 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2014-8
-
-
830
View
-
0
Download
-
6
Crossref
-
 Abstract
 PDF
-
Increasing pressure in antibiotic resistance and the requirement for the design of new vaccines are the objectives of clarifying the putative virulence factors in pneumococcal infection. In this study, the putative threonine dehydratase sp0454 was inactivated by erythromycin-resistance cassette replacement in Streptococcus pneumoniae CMCC 31203 strain. The sp0454 mutant was tested for cell growth, adherence, colonization, and virulence in a murine model. The Δsp0454 mutant showed decreased ability for colonization and impaired ability to adhere to A549 cells. However, the SP0454 polypeptide or its antiserum did not affect pneumococcal CMCC 31203 adhesion to A549 cells. The sp0454 deletion mutant was less virulent in a murine intranasal infection model. Real-time RT-PCR analysis revealed significant decrease of the pneumococcal surface antigen A expression in the sp0454 mutant. These results suggest that SP0454 contributes to virulence and colonization, which could be explained in part by modulating the expression of other virulence factors, such as psaA in pneumococcal infection.
-
Citations
Citations to this article as recorded by  - Pneumococcal live attenuated vaccine: strategies, challenges and future perspectives
Marina Yusoff, Chew Chieng Yeo, Malik Amonov
Exploration of Immunology.2026;[Epub] CrossRef - Dual RNA-Seq of Flavobacterium psychrophilum and Its Outer Membrane Vesicles Distinguishes Genes Associated with Susceptibility to Bacterial Cold-Water Disease in Rainbow Trout (Oncorhynchus mykiss)
Pratima Chapagain, Ali Ali, Mohamed Salem
Pathogens.2023; 12(3): 436. CrossRef - Heterologous prime-boost immunization with live SPY1 and DnaJ protein of Streptococcus pneumoniae induces strong Th1 and Th17 cellular immune responses in mice
Yulan Qiu, Xuemei Zhang, Hong Wang, Xinyuan Zhang, Yunjun Mo, Xiaoyu Sun, Jichao Wang, Yibing Yin, Wenchun Xu
Journal of Microbiology.2017; 55(10): 823. CrossRef - Mucosal Immunization with the Live Attenuated Vaccine SPY1 Induces Humoral and Th2-Th17-Regulatory T Cell Cellular Immunity and Protects against Pneumococcal Infection
Xiuyu Xu, Hong Wang, Yusi Liu, Yiping Wang, Lingbing Zeng, Kaifeng Wu, Jianmin Wang, Feng Ma, Wenchun Xu, Yibing Yin, Xuemei Zhang, A. Camilli
Infection and Immunity.2015; 83(1): 90. CrossRef - A Novel Protein, RafX, Is Important for Common Cell Wall Polysaccharide Biosynthesis in Streptococcus pneumoniae: Implications for Bacterial Virulence
Kaifeng Wu, Jian Huang, Yanqing Zhang, Wenchun Xu, Hongmei Xu, Libin Wang, Ju Cao, Xuemei Zhang, Yibing Yin
Journal of Bacteriology.2014; 196(18): 3324. CrossRef - Serotype-independent protection against pneumococcal infections elicited by intranasal immunization with ethanol-killed pneumococcal strain, SPY1
Xiuyu Xu, Jiangping Meng, Yiping Wang, Jie Zheng, Kaifeng Wu, Xuemei Zhang, Yibing Yin, Qun Zhang
Journal of Microbiology.2014; 52(4): 315. CrossRef
|
MSK
