- Relationship Between Mycotoxin Production and Gene Expression in Fusarium graminearum Species Complex Strains Under Various Environmental Conditions
-
Wenwen Huang , Ping Zhou , Guanghui Shen , Tao Gao , Xin Liu , Jianrong Shi , Jianhong Xu , Jianbo Qiu
-
J. Microbiol. 2023;61(5):525-542. Published online May 2, 2023
-
DOI: https://doi.org/10.1007/s12275-023-00046-4
-
-
1,224
View
-
5
Download
-
17
Web of Science
-
13
Crossref
-
 Abstract
 PDF
-
The Fusarium graminearum species complex (FGSC) can produce various mycotoxins and is a major concern for food
quantity and quality worldwide. In this study, we determined the effects of water activity (
aw), temperature, incubation time
and their interactions on mycotoxin accumulation and the expression levels of biosynthetic genes in FGSC strains from
maize samples in China. The highest deoxynivalenol (DON), 3-acetyldeoxynivalenol(3ADON) and 15-acetyldeoxynivalenol
(15ADON) levels of the F. boothii and F. graminearum strains were observed at 0.98 aw/
30 °C or 0.99 aw/
25 °C. F. asiaticum
and F. meridionale reached maximum nivalenol (NIV) and 4-acetylnivalenol (4ANIV) contents at 0.99 aw
and 30 °C. With
the extension of the incubation time, the concentrations of DON and NIV gradually increased, while those of their derivatives
decreased. F. boothii, F. meridionale and one F. asiaticum strain had the highest zearalenone (ZEN) values at 0.95 aw
and 25 °C, while the optimum conditions for the other F. asiaticum strain and F. graminearum were 0.99 aw
and 30 °C. Four
genes associated with trichothecene and zearalenone synthesis were significantly induced under higher water stress in the
early stage of production. The results indicated independence of mycotoxin production and gene expression, as maximum
amounts of these toxic metabolites were observed at higher aw
in most cases. This study provides useful information for the
monitoring and prevention of such toxins entering the maize production chain.
-
Citations
Citations to this article as recorded by  - A Study on the Spatial Distribution of Zearalenone and Deoxynivalenol in Oat Bran
Honglei Qu, Pengshuai Li, Xiaoping Rong, Zhonghao Liu, Ruifen Kang, Wenqiong Chai, Qiugang Ma
Foods.2026; 15(5): 807. CrossRef - The impact of environmental variations (temperature and humidity) on Fusarium graminearum in broiler and pig feed
Osias Thabo Ramoeletsi, Olerato Tumiso Sebolao, Martha Cebile Jobe, Mulunda Mwanza
Frontiers in Fungal Biology.2026;[Epub] CrossRef -
A multiplant transcriptomic atlas reveals conserved and lineage-specific defense architectures in response to
Botrytis cinerea
Ritu Singh, Anna Jo Muhich, Cloe Tom, Celine Caseys, Daniel J. Kliebenstein
Proceedings of the National Academy of Sciences.2026;[Epub] CrossRef - Apicidin is a key virulence determinant of Fusarium asiaticum in wheat-rice cropping systems
Xin Liu, Kainan Li, Jianbo Qiu, Tao Gao, Yi Shi, Guanghui Shen, Yunyun Zhou, Shilong Hu, Xuanlong Ruan, Jingya Wan, Yin-Won Lee, Qin Gu, Jae Yun Lim, Jeong-Ah Seo, Jianrong Shi, Wenwu Ye, Jianhong Xu
Science China Life Sciences.2026; 69(8): 2892. CrossRef - Pathogenicity and Toxigenic Chemotype Analysis of Fusarium Species Causing Maize Stalk Rot and Evaluation of Trichoderma for Biocontrol and Mycotoxin Suppression
Nan Chen
Journal of Fungi.2026; 12(7): 505. CrossRef - Integrated high-throughput sequencing and UFLC-MS/MS to explore the correlation between fungal diversity and mycotoxins in Coix seed
Yujiao Hou, Xiaofang Liao, Lan Yao, Fan Zhou, Limei Wen, Minghan Yang, Wei Lan, Jianhua Yang, Weijun Kong
Journal of Food Composition and Analysis.2026; 158: 109445. CrossRef - How temperature reshapes interactions in mixed cultures of mycotoxigenic fungi
Darina Balková, Marco Camardo Leggieri, Paola Battilani
Frontiers in Microbiology.2026;[Epub] CrossRef - Highly Conserved
Allelic Substitutions S202F, L244P,
and H248P/R/Y in FsSdhB and A83 V in FsSdhC1 Trigger Gradual Resistance
to Pydiflumetofen in Fusarium solani
Lingling Wei, Liguo Tan, Yinhong Liu, Weimeng Huang, Lu Ren, Xiaojun Zhao
Journal of Agricultural
and Food Chemistry.2026; 74(35): 27389. CrossRef - Geographic variations in the Fusarium species and toxins associated with maize ear rot in China
Jianbo Qiu, Can Jiang, Shufang Wang, Can He, Daoming Chen, Jing Lan, Jianhong Xu, Yin-Won Lee, Jianrong Shi
International Journal of Food Microbiology.2025; 436: 111208. CrossRef - Antagonistic activity of two yeasts against Penicillium expansum in stone fruits
Paula Tejero, Alicia Rodríguez, Alberto Martín, Sandra Olmo, Alejandro Hernández
Journal of the Science of Food and Agriculture.2025; 105(15): 8878. CrossRef - Ecophysiology of Fusarium graminearum and Fusarium proliferatum on sorghum grains
Ana Belén Corallo, Agustina del Palacio, María Oliver, Silvina Stewart, Lucía Pareja, Dinorah Pan
International Journal of Food Microbiology.2025; 442: 111380. CrossRef - Biosynthesis Regulation of Secondary Metabolite Production in Fusarium Fungi
Prosper Amuzu, Xiaoqian Pan, Xuwen Hou, Yu Li, Jiahang Sun, Yujun Huang, Pengfei Wang, Liyao Liu, Daowan Lai, Ligang Zhou
Journal of Fungi.2025; 11(11): 820. CrossRef - Application of MOX Sensors to Determine the Emission of Volatile Compounds in Corn Groats as a Function of Vertical Pressure in the Silo and Moisture Content of the Bed
Robert Rusinek, Aleksandra Żytek, Mateusz Stasiak, Joanna Wiącek, Marek Gancarz
Sensors.2024; 24(7): 2187. CrossRef
- FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum
-
Xin Liu , Yichen Jiang , Yinghui Zhang , Mingzheng Yu , Hongjun Jiang , Jianhong Xu , Jianrong Shi
-
J. Microbiol. 2019;57(8):694-703. Published online May 11, 2019
-
DOI: https://doi.org/10.1007/s12275-019-9123-6
-
-
959
View
-
0
Download
-
16
Web of Science
-
16
Crossref
-
Abstract
PDF
-
Dihydroxyacid dehydratase (DHAD), encoded by ILV3, catalyses
the third step in the biosynthetic pathway of branchedchain
amino acids (BCAAs), which include isoleucine (Ile),
leucine (Leu), and valine (Val). Enzymes involved in BCAA
biosynthesis exist in bacteria, plants, and fungi but not in
mammals and are therefore attractive targets for antimicrobial
or herbicide development. In this study, three paralogous
ILV3 genes (FgILV3A, FgILV3B, and FgILV3C) were identified
in the genome of Fusarium graminearum, the causal
agent of Fusarium head blight (FHB). Deletion of FgILV3A
alone or combined with FgILV3B or FgILV3C indicated an
important role for FgILV3A in BCAA biosynthesis. FgILV3A
deletion mutants lost the ability to grow on medium lacking
amino acids. Exogenous supplementation of 1 mM Ile and
Val rescued the auxotrophy of ΔFgIlv3A, though 5 mM was
required to recover the growth defects in ΔFgIlv3AB and
ΔFgIlv3AC strains, indicating that FgIlv3b and FgIlv3c exhibit
redundant but accessory roles with FgIlv3a in BCAA
biosynthesis. The auxotrophy of ΔFgIlv3A resulted in pleiotropic
defects in aerial hyphal growth, in conidial formation
and germination, and in aurofusarin accumulation. In addition,
the mutants showed reduced virulence and deoxynivalenol
production. Overall, our study demonstrates that
FgIlv3a is crucial for BCAA biosynthesis in F. graminearum and a candidate fungicide target for FHB management.
-
Citations
Citations to this article as recorded by - Beyond differential expression: a machine learning approach to identify regulatory hubs in Moniliophthora roreri infection of cacao
Seunghyun Lim, Sunchung Park, Jishnu Bhatt, Jae Hee Jang, Dapeng Zhang, Lyndel W. Meinhardt, Ezekiel Ahn
European Journal of Plant Pathology.2026; 174(4): 645. CrossRef - Metabolic engineering of Pichia pastoris for high-efficiency production of branched-chain amino acids -enriched single-cell protein
Cancan Dong, Kai Hong, Fan Wu, Yanfeng Wang, Youyan Rong, Xin Wu, Jian Zhang, Le Gao
Bioresource Technology.2026; 444: 133988. CrossRef - Transcription Activator FgDDT Interacts With FgISW1 to Regulate Fungal Development and Pathogenicity in the Global Pathogen Fusarium graminearum
Xiaozhen Zhao, Yuxin Qiu, Aning Jiang, Yan Huang, Peixue Ma, Bingqin Yuan, Li Chen, Chengqi Zhang
Molecular Plant Pathology.2025;[Epub] CrossRef - Biocontrol efficiency and potential mechanism of dimethyl trisulfide against postharvest Diplodia stem-end rot in pummelo (Citrus maxima)
Yankun Lin, Shun Feng, Haijin Cai, Haibo Wang, Xiuli Zhang, Fangfang Wu, Shuoyu An, Xinguo Li
Postharvest Biology and Technology.2025; 230: 113792. CrossRef - Pseudomonas from Potato Inhibited the Pathogenicity of Verticillium nonalfalfae through the BCAA Biosynthesis Pathway
Mengyuan Wan, Xinlong Chen, Xiaoxi Yi, Yujia Fu, Yonggang Li, Dianqiu Lv
Journal of Agricultural and Food Chemistry.2025; 73(35): 21818. CrossRef - AflaILVB/G/I and AflaILVD are involved in mycelial production, aflatoxin biosynthesis, and fungal virulence in Aspergillus flavus
Yarong Zhao, Chulan Huang, Rui Zeng, Peirong Chen, Kaihang Xu, Xiaomei Huang, Xu Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - Histone H3 N-Terminal Lysine Acetylation Governs Fungal Growth, Conidiation, and Pathogenicity through Regulating Gene Expression in Fusarium pseudograminearum
Hang Jiang, Lifang Yuan, Liguo Ma, Kai Qi, Yueli Zhang, Bo Zhang, Guoping Ma, Junshan Qi
Journal of Fungi.2024; 10(6): 379. CrossRef - Identification and Characterization of an Antifungal Gene Mt1 from Bacillus subtilis by Affecting Amino Acid Metabolism in Fusarium graminearum
Pei Song, Wubei Dong
International Journal of Molecular Sciences.2023; 24(10): 8857. CrossRef - Branched-chain amino acid biosynthesis in fungi
Gary Jones, Jane Usher, Joel T. Steyer, Richard B. Todd
Essays in Biochemistry.2023; 67(5): 865. CrossRef - FgLEU1 Is Involved in Leucine Biosynthesis, Sexual Reproduction, and Full Virulence in Fusarium graminearum
Shaohua Sun, Mingyu Wang, Chunjie Liu, Yilin Tao, Tian Wang, Yuancun Liang, Li Zhang, Jinfeng Yu
Journal of Fungi.2022; 8(10): 1090. CrossRef - Acetolactate synthases regulatory subunit and catalytic subunit genes VdILVs are involved in BCAA biosynthesis, microscletotial and conidial formation and virulence in Verticillium dahliae
ShengNan Shao, Biao Li, Qi Sun, PeiRu Guo, YeJuan Du, JiaFeng Huang
Fungal Genetics and Biology.2022; 159: 103667. CrossRef - Molecular targets for antifungals in amino acid and protein biosynthetic pathways
Aleksandra Kuplińska, Kamila Rząd
Amino Acids.2021; 53(7): 961. CrossRef - MoCpa1-mediated arginine biosynthesis is crucial for fungal growth, conidiation, and plant infection of Magnaporthe oryzae
Osakina Aron, Min Wang, Anjago Wilfred Mabeche, Batool Wajjiha, Meiqin Li, Shuai Yang, Haixia You, Yan Cai, Tian Zhang, Yunxi Li, Baohua Wang, Dongmei Zhang, Zonghua Wang, Wei Tang
Applied Microbiology and Biotechnology.2021; 105(14-15): 5915. CrossRef - Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid
Marta J. Fiołka, Paulina Czaplewska, Sylwia Wójcik-Mieszawska, Aleksandra Lewandowska, Kinga Lewtak, Weronika Sofińska-Chmiel, Tomasz Buchwald
Scientific Reports.2021;[Epub] CrossRef - The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum
Tao Gao, Dan He, Xin Liu, Fang Ji, Jianhong Xu, Jianrong Shi
Food Production, Processing and Nutrition.2020;[Epub] CrossRef -
The Intermediates in Branched-Chain Amino Acid Biosynthesis Are Indispensable for Conidial Germination of the Insect-Pathogenic Fungus Metarhizium
robertsii
Feifei Luo, Hongxia Zhou, Xue Zhou, Xiangyun Xie, You Li, Fenglin Hu, Bo Huang, Karyn N. Johnson
Applied and Environmental Microbiology.2020;[Epub] CrossRef
|
MSK
