- Identification of a New Bacillus licheniformis Strain Producing a Bacteriocin-Like Substance
-
Yaoqi Guo , Zhanqiao Yu , Jianhua Xie , Rijun Zhang
-
J. Microbiol. 2012;50(3):452-458. Published online June 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2051-3
-
-
985
View
-
6
Download
-
13
Crossref
-
 Abstract
 PDF
-
The emergence of antibiotic resistance has spurred a great number of studies for development of new antimicrobials in the past decade. The purpose of this study was to screen environmental samples for Bacillus strains producing potent antimicrobial agents. A new strain, which showed strong antimicrobial activity against Staphylococcus aureus and Salmonella enterica ser. Pullorum, was isolated from soil and designated as B116. This new isolate was identified as Bacillus licheniformis by morphological, biochemical and genetic analyses. The production of bacteriocin-like substance (BLS) started at early exponential phase and achieved highest level at early stationary phase. The BLS was precipitated by ammonium sulfate and its molecular mass was determined as ~4 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Culture supernatant of the new isolate exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria, including Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Micrococcus luteus, Escherichia coli, and Salmonella spp. The BLS was resistant to heat, acid and alkaline treatment. Activity of the BLS was totally lost after digestion by pronase and partially lost after digestion by papain and lipase. The new isolate and relevant BLS are potentially useful in food and feed applications.
-
Citations
Citations to this article as recorded by  - Calcium and magnesium ions removal by different bacteria strains: Implications for limitation of lime-scale formation during seawater desalination
Ruirui Meng, Zuozhen Han, Wen Nie, Xiangwei Cheng, Chao Han, Yu Han, Maurice E. Tucker
Journal of Cleaner Production.2025; 525: 146521. CrossRef - Linking environmental cues to genomic responses for enhanced production and recovery of Bacillus bacteriocins via ammonium sulphate precipitation: a review
Nelisiwe Busisiwe Phakathi, Adrian Abrahams, Bhekisisa Dlamini
International Journal of Food Science and Technology.2025;[Epub] CrossRef - Metagenomic nanopore sequencing for exploring the nature of antimicrobial metabolites of Bacillus haynesii
Mohamed A. Eltokhy, Bishoy T. Saad, Wafaa N. Eltayeb, Mohammad Y. Alshahrani, Sahar M R Radwan, Khaled M. Aboshanab, Mohamed S. E. Ashour
AMB Express.2024;[Epub] CrossRef - Whole genome sequence and LC-Mass for identifying antimicrobial metabolites of Bacillus licheniformis endophyte
Nourhan K. Soliman, Ahmad M. Abbas, Wafaa N. El tayeb, Mohammad Y. Alshahrani, Khaled M. Aboshanab
AMB Express.2024;[Epub] CrossRef - Bacillus licheniformis: A Producer of Antimicrobial Substances, including Antimycobacterials, Which Are Feasible for Medical Applications
Margarita O. Shleeva, Daria A. Kondratieva, Arseny S. Kaprelyants
Pharmaceutics.2023; 15(7): 1893. CrossRef - Bacillus sp. Bacteriocins: Natural Weapons against Bacterial Enemies
Jessica Vaca, Aurelio Ortiz, Estibaliz Sansinenea
Current Medicinal Chemistry.2022; 29(12): 2093. CrossRef - Impact of oil contamination on ecological functions of peat soils from West Siberia of Russia
Ekaterina I. Kovaleva, Sergey Ya. Trofimov, Cheng Zhongqi
Journal of Environmental Quality.2021; 50(1): 49. CrossRef - Biotechnological applications of Bacillus licheniformis
Andrea Muras, Manuel Romero, Celia Mayer, Ana Otero
Critical Reviews in Biotechnology.2021; 41(4): 609. CrossRef - Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers
En-Ru Lin, Yeong-Hsiang Cheng, Felix Shih-Hsiang Hsiao, Witold S. Proskura, Andrzej Dybus, Yu-Hsiang Yu
Revista Brasileira de Zootecnia.2019;[Epub] CrossRef - Secondary metabolites from Bacillus amyloliquefaciens isolated from soil can kill Burkholderia pseudomallei
Patcharaporn Boottanun, Chotima Potisap, Julian G. Hurdle, Rasana W. Sermswan
AMB Express.2017;[Epub] CrossRef - Evaluation of two probiotics used during farm production of white shrimp Litopenaeus vannamei
(Crustacea: Decapoda)
Ramón Franco, Leonardo Martín, Amilcar Arenal, Dayamí Santiesteban, Jorge Sotolongo, Hector Cabrera, Jaime Mejías, George Rodríguez, Angela G Moreno, Eulogio Pimentel, Nestor M Castillo
Aquaculture Research.2017; 48(4): 1936. CrossRef - Investigation of the Antimicrobial Activity of Bacillus licheniformis Strains Isolated from Retail Powdered Infant Milk Formulae
Avelino Alvarez-Ordóñez, Máire Begley, Tanya Clifford, Thérèse Deasy, Kiera Considine, Paula O’Connor, R. Paul Ross, Colin Hill
Probiotics and Antimicrobial Proteins.2014; 6(1): 32. CrossRef - Chemical and genetic characterization of bacteriocins: antimicrobial peptides for food safety
Abigail B Snyder, Randy W Worobo
Journal of the Science of Food and Agriculture.2014; 94(1): 28. CrossRef
|
MSK
