Journal of Microbiology

Jiang Gu

2 Articles

Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China: Xiao W. Ji , Ya L. Liao , Ye F. Zhu , Hai G. Wang , Ling Gu , Jiang Gu , Chen Dong , Hong L. Ding , Xu H. Mao , Feng C. Zhu , Quan M. Zou; J. Microbiol. 2010;48(6):849-855. Published online January 9, 2011; DOI: https://doi.org/10.1007/s12275-010-0132-8

824 View
0 Download
7 Crossref

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7 strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

Citations

Citations to this article as recorded by

Antimicrobial resistance and associated risk factors in Escherichia coli isolated from Peruvian dogs: A focus on extended-spectrum β-lactamases and colistin
Margot Ventura, Rosario Oporto-Llerena, Kathya Espinoza, Fernando Guibert, Antonio M. Quispe, Nidia Vilar, María López, Beatriz Rojo-Bezares, Yolanda Sáenz, Joaquim Ruiz, Maria J. Pons
Veterinary World.2024; : 880. CrossRef
Traditional marketed meats as a reservoir of multidrug-resistant Escherichia coli
Fernando Guibert, Kathya Espinoza, Clara Taboada-Blanco, Carla A. Alonso, Rosario Oporto, Angie K. Castillo, Beatriz Rojo-Bezares, María López, Yolanda Sáenz, Maria J. Pons, Joaquim Ruiz
International Microbiology.2023; 28(S1): 27. CrossRef
First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China
Zhanqiang Su, Panpan Tong, Ling Zhang, Mengmeng Zhang, Dong Wang, Kaiqi Ma, Yi Zhang, Yingyu Liu, Lining Xia, Jinxin Xie
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Multilocus-based phylogenetic analysis of extended-spectrum beta-lactamase Escherichia coli O157:H7 uncovers related strains between agriculture and nearby water sources
Cecilia Mahlatse Raseala, Mutshiene Deogratias Ekwanzala, Maggy Ndombo Benteke Momba
Journal of Infection and Public Health.2020; 13(12): 1899. CrossRef
Molecular and Phenotypic Characteristics ofEscherichia coliIsolates from Farmed Minks in Zhucheng, China
Jianhua Qiu, Zhiyu Jiang, Zijing Ju, Xiaonan Zhao, Jie Yang, Huijun Guo, Shuhong Sun
BioMed Research International.2019; 2019: 1. CrossRef
Molecular Characterization of EnterohemorrhagicE. coliO157 Isolated from Animal Fecal and Food Samples in Eastern China
Shaohui Wang, Shuxiao Zhang, Zhe Liu, Pingping Liu, Zixue Shi, Jianchao Wei, Donghua Shao, Beibei Li, Zhiyong Ma
The Scientific World Journal.2014; 2014: 1. CrossRef
Scientific Opinion on the evaluation of molecular typing methods for major food‐borne microbiological hazards and their use for attribution modelling, outbreak investigation and scanning surveillance: Part 1 (evaluation of methods and applications)

EFSA Journal.2013;[Epub] CrossRef

Fusion Expression and Immunogenicity of EHEC EspA-Stx2A1 Protein: Implications for the Vaccine Development: Yan Cheng , Youjun Feng , Ping Luo , Jiang Gu , Shu Yu , Wei-jun Zhang , Yan-qing Liu , Qing-xu Wang , Quan-ming Zou , Xu-hu Mao; J. Microbiol. 2009;47(4):498-505. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0116-8

805 View
0 Download
20 Crossref

Abstract PDF

Shiga toxin 2 (Stx2) is a major virulence factor for enterohemorrhagic Escherichia coli (EHEC), which is encoded by λ lysogenic phage integrated into EHEC chromosome. Stx2A1, A1 subunit of Stx2 toxin has gathered extensive concerns due to its potential of being developed into a vaccine candidate. However, the substantial progress is hampered in part for the lack of a suitable in vitro expression system. Here we report use of the prokaryotic system pET-28a::espA-Stx2A1/BL21 to carry out the fusion expression of Stx2A1 which is linked to E. coli secreted protein A (EspA) at its N-terminus. Under the IPTG induction, EspA- Stx2A1 fusion protein in the form of inclusion body was obtained successfully, whose expression level can reach about 40% of total bacterial protein at 25°C, much higher than that at 37°C. Western blot test suggested the refolded fusion protein is of excellent immuno-reactivity with both monoclonal antibodies, which are specific to EspA and Stx2A1, respectively. Anti-sera from Balb/c mice immunized with the EspA-Stx2A1 fusion protein were found to exhibit strong neutralization activity and protection capability in vitro and in vivo. These data have provided a novel feasible method to produce Stx2A1 in large scale in vitro, which is implicated for the development of multivalent subunit vaccines candidate against EHEC O157:H7 infections.

Citations

Citations to this article as recorded by

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen
Pravil Pokharel, Sabin Dhakal, Charles M. Dozois
Microorganisms.2023; 11(2): 344. CrossRef
In silico design, production and immunization evaluation of a recombinant bivalent fusion protein candidate vaccine against E. coli O157:H7
Hossein Samiei, Shahram Nazarian, Abass Hajizade, Emad Kordbacheh
International Immunopharmacology.2023; 114: 109464. CrossRef
Oral Administration with Live Attenuated Citrobacter rodentium Protects Immunocompromised Mice from Lethal Infection
Shuyu Wang, Xue Xia, Yue Liu, Fengyi Wan, Guy H. Palmer
Infection and Immunity.2022;[Epub] CrossRef
Diagnosis and Treatment for Shiga Toxin-Producing Escherichia coli Associated Hemolytic Uremic Syndrome
Yang Liu, Hatim Thaker, Chunyan Wang, Zhonggao Xu, Min Dong
Toxins.2022; 15(1): 10. CrossRef
Immunization of mice with chimeric antigens displaying selected epitopes confers protection against intestinal colonization and renal damage caused by Shiga toxin-producing Escherichia coli
David A. Montero, Felipe Del Canto, Juan C. Salazar, Sandra Céspedes, Leandro Cádiz, Mauricio Arenas-Salinas, José Reyes, Ángel Oñate, Roberto M. Vidal
npj Vaccines.2020;[Epub] CrossRef
Treatment Strategies for Infections With Shiga Toxin-Producing Escherichia coli
Sabrina Mühlen, Petra Dersch
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Oral immunization of mice with Lactococcus lactis expressing Shiga toxin truncate confers enhanced protection against Shiga toxins of Escherichia coli O157:H7 and Shigella dysenteriae
Sagi Sreerohini, Konduru Balakrishna, Manmohan Parida
APMIS.2019; 127(10): 671. CrossRef
Mucosal and systemic responses of immunogenic vaccines candidates against enteric Escherichia coli infections in ruminants: A review
A. Lawan, F.F.A. Jesse, U.H. Idris, M.N. Odhah, M. Arsalan, N.A. Muhammad, K.R. Bhutto, I.D. Peter, G.A. Abraham, A.H. Wahid, M.L. Mohd-Azmi, M. Zamri-Saad
Microbial Pathogenesis.2018; 117: 175. CrossRef
Intestinal Pathogenic Escherichia coli: Insights for Vaccine Development
Maricarmen Rojas-Lopez, Ricardo Monteiro, Mariagrazia Pizza, Mickaël Desvaux, Roberto Rosini
Frontiers in Microbiology.2018;[Epub] CrossRef
Current pathogenic Escherichia coli foodborne outbreak cases and therapy development
Shih-Chun Yang, Chih-Hung Lin, Ibrahim A. Aljuffali, Jia-You Fang
Archives of Microbiology.2017; 199(6): 811. CrossRef
In silico analysis of Shiga toxins (Stxs) to identify new potential vaccine targets for Shiga toxin-producing Escherichia coli
Maryam Golshani, Mana Oloomi, Saeid Bouzari
In Silico Pharmacology.2017;[Epub] CrossRef
Production and purification of an untagged recombinant pneumococcal surface protein A (PspA4Pro) with high-purity and low endotoxin content
Douglas B. Figueiredo, Eneas Carvalho, Mauricio P. Santos, Stefanie Kraschowetz, Rafaela T. Zanardo, Gilson Campani, Gabriel G. Silva, Cíntia R. Sargo, Antonio Carlos L. Horta, Roberto de C. Giordano, Eliane N. Miyaji, Teresa C. Zangirolami, Joaquin Cabre
Applied Microbiology and Biotechnology.2017; 101(6): 2305. CrossRef
Escherichia coli outer membrane protein F (OmpF): an immunogenic protein induces cross-reactive antibodies against Escherichia coli and Shigella
Xiao Wang, Da Teng, Qingfeng Guan, Ruoyu Mao, Ya Hao, Xiumin Wang, Junhu Yao, Jianhua Wang
AMB Express.2017;[Epub] CrossRef
Vaccines for viral and bacterial pathogens causing acute gastroenteritis: Part II: Vaccines for Shigella , Salmonella, enterotoxigenic E. coli (ETEC) enterohemorragic
Miguel O’Ryan, Roberto Vidal, Felipe del Canto, Juan Carlos Salazar, David Montero
Human Vaccines & Immunotherapeutics.2015; 11(3): 601. CrossRef
Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice
Qingfeng Guan, Xiao Wang, Xiumin Wang, Da Teng, Ruoyu Mao, Yong Zhang, Jianhua Wang
Applied Microbiology and Biotechnology.2015; 99(13): 5451. CrossRef
Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model
Xiao Wang, Qingfeng Guan, Xiumin Wang, Da Teng, Ruoyu Mao, Junhu Yao, Jianhua Wang
Process Biochemistry.2015; 50(8): 1194. CrossRef
Comparative Genomics and Immunoinformatics Approach for the Identification of Vaccine Candidates for Enterohemorrhagic Escherichia coli O157:H7
Víctor A. García-Angulo, Anjana Kalita, Mridul Kalita, Luis Lozano, Alfredo G. Torres, S. M. Payne
Infection and Immunity.2014; 82(5): 2016. CrossRef
Advances in the development of enterohemorrhagic Escherichia coli vaccines using murine models of infection
Victor A. Garcia-Angulo, Anjana Kalita, Alfredo G. Torres
Vaccine.2013; 31(32): 3229. CrossRef
Evaluation of hha and hha sepB mutant strains of Escherichia coli O157:H7 as bacterins for reducing E. coli O157:H7 shedding in cattle
Vijay K. Sharma, Evelyn A. Dean-Nystrom, Thomas A. Casey
Vaccine.2011; 29(31): 5078. CrossRef
Vaccination of attenuated EIS-producing Salmonella induces protective immunity against enterohemorrhagic Escherichia coli in mice
Jiang Gu, Yuanyuan Ning, Haiguang Wang, Daping Xiao, Bin Tang, Ping Luo, Yan Cheng, Mingming Jiang, Na Li, Quanming Zou, Xuhu Mao
Vaccine.2011; 29(43): 7395. CrossRef

Author index