Journal of Microbiology

Ji-Won Lee

2 Articles

Detection of Hepatitis A Virus from Oyster by Nested PCR Using Efficient Extraction and Concentration Method: Duwoon Kim , Seok-Ryel Kim , Ki-Sung Kwon , Ji-Won Lee , Myung-Joo Oh; J. Microbiol. 2008;46(4):436-440. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0131-1

700 View
0 Download
21 Crossref

The molecular methods using polymerase chain reaction have been proposed as useful tools for the identification of viral pathogens in food and water. However, the PCR-based methods are highly dependent on the methods of virus concentration and nucleic acid purification due to the low sensitivity of PCR in the presence of PCR inhibitors. We developed TPTT [tris elution buffer-PEG-TRIzol-poly(dT) magnetic bead] protocol in order to detect hepatitis A virus (HAV) inoculated in oyster digestive glands. The detection limit of HAV precipitated with zirconium hydroxide was 105 fold less sensitive in a nested PCR than that precipitated the HAV supernatant twice with PEG/NaCl (16% polyethylene glycol 6,000, 0.525 M NaCl) in a 1:2 (v/v) ratio, which provided an efficient detection of 0.0148 PFU/g from approximately 0.05 g of oyster homogenate. This method is efficient for potential use in the detection of HAV from shellfish and is more sensitive than most currently published tests.

Citations

Citations to this article as recorded by

Differences in the effectiveness of the high-efficient concentrated pretreatment method on the norovirus detection in oysters and mussels
Yijing Li, Liang Xue, Junshan Gao, Weicheng Cai, Yanhui Liang, Zilei Zhang, Linping Wang, Xiaojing Hong, Luobing Meng, Mingfang Xu, Qingping Wu, Jumei Zhang
International Journal of Food Microbiology.2023; 384: 109957. CrossRef
Application of Concanavalin A–Linked Magnetic Beads for the Detection of Hepatitis A Virus
Sang-Mu Ko, Se-Young Cho, Myung-Joo Oh, Joseph Kwon, Bipin Vaidya, Duwoon Kim
Journal of Food Protection.2018; 81(12): 1997. CrossRef
Molecular Detection of theSxtaGene from Saxitoxin-ProducingAlexandrium minutumin Commercial Oysters
Hazel Farrell, Wayne O'Connor, Frank Seebacher, D. Tim Harwood, Shauna Murray
Journal of Shellfish Research.2016; 35(1): 169. CrossRef
Molecular Detection of Foodborne Pathogens: A Rapid and Accurate Answer to Food Safety
Manisha Mangal, Sangita Bansal, Satish K. Sharma, Ram K. Gupta
Critical Reviews in Food Science and Nutrition.2016; 56(9): 1568. CrossRef
Detection of Hepatitis A Virus in Seeded Oyster Digestive Tissue by Ricin A-Linked Magnetic Separation Combined with Reverse Transcription PCR
Sang-Mu Ko, Bipin Vaidya, Joseph Kwon, Hee-Min Lee, Myung-Joo Oh, Tai-Sun Shin, Se-Young Cho, Duwoon Kim
Journal of Food Protection.2015; 78(5): 1046. CrossRef
A rapid, sensitive and selective electrochemical biosensor with concanavalin A for the preemptive detection of norovirus
Sung A Hong, Joseph Kwon, Duwoon Kim, Sung Yang
Biosensors and Bioelectronics.2015; 64: 338. CrossRef
Rapid detection of the hepatitis a virus from fresh lettuce using immunomagnetic separation and quantum dots assay
Hee-Min Lee, Joseph Kwon, Jong-Soon Choi, Yong-Gwan Won, Eun-Sun Kim, Jae-Keun Chung, Min-Ji Kim, Duwoon Kim
Korean Journal of Food Preservation.2014; 21(2): 170. CrossRef
Development of Lectin-Linked Immunomagnetic Separation for the Detection of Hepatitis A Virus
Sang-Mu Ko, Joseph Kwon, Bipin Vaidya, Jong Choi, Hee-Min Lee, Myung-Joo Oh, Hyeun-Jong Bae, Se-Young Cho, Kyung-Seo Oh, Duwoon Kim
Viruses.2014; 6(3): 1037. CrossRef
Rapid Detection of Norovirus from Fresh Lettuce Using Immunomagnetic Separation and a Quantum Dots Assay
HEE-MIN LEE, JOSEPH KWON, JONG-SOON CHOI, KYEONG-HWAN LEE, SUNG YANG, SANG-MU KO, JAE-KEUN CHUNG, SE-YOUNG CHO, DUWOON KIM
Journal of Food Protection.2013; 76(4): 707. CrossRef
Comparative evaluation of a novel TaqMan real-time reverse transcription–polymerase chain reaction assay for hepatitis A virus detection
Feng Qiu, Huihui Zheng, Yao Yi, Zhiyuan Jia, Jingyuan Cao, Shengli Bi
Journal of International Medical Research.2013; 41(2): 427. CrossRef
Therapeutic Effect of Carboxymethylated and Quanternized Chitosan on Insulin Resistance in High-Fat-Diet-Induced Rats and 3T3-L1 Adipocytes
Xiaofei Liu, Fan Yang, Tao Song, Anrong Zeng, Qi Wang, Zhong Sun, Jun Shen
Journal of Biomaterials Science, Polymer Edition.2012; 23(10): 1271. CrossRef
Use of IMS-RT-PCR for the Rapid Isolation and Detection of Hepatitis A Virus from the Swine Feces
Hee-Min Lee, Duwoon Kim
Korean Journal of Food Science and Technology.2012; 44(5): 638. CrossRef
Rapid Detection Method for Hepatitis A Virus from Lettuce by a Combination of Filtration and Integrated Cell Culture-Real-Time Reverse Transcription PCR
Ji-Yeon Hyeon, Jung-Whan Chon, Chankyu Park, Jung-Bok Lee, In-Soo Choi, Moo-Sang Kim, Kun-Ho Seo
Journal of Food Protection.2011; 74(10): 1756. CrossRef
Rapid Detection and Limitations of Molecular Techniques
John J. Maurer
Annual Review of Food Science and Technology.2011; 2(1): 259. CrossRef
Synthesis of Carboxymethylated and Quaternized Chitosans and Their Therapeutic Effect on Nonalcoholic Fatty Liver Disease
Xiaofei Liu, Fan Yang, Tao Song, Anrong Zeng, Qi Wang, Zhong Sun, Jun Shen
Journal of Agricultural and Food Chemistry.2011; 59(19): 10683. CrossRef
Effects of an Oxidative Agent and Lectins on the Binding Inhibition of Recombinant Hepatitis A Virus Proteins to Oyster Digestive Tissues
Sang-Mu Ko, Jong-Oh Kim, Myung-Joo Oh, Duwoon Kim
Journal of Food Protection.2011; 74(1): 157. CrossRef
Effects of chitosan, O-carboxymethyl chitosan and N-[(2-hydroxy-3-N,N-dimethylhexadecyl ammonium)propyl]chitosan chloride on lipid metabolism enzymes and low-density-lipoprotein receptor in a murine diet-induced obesity
Xiaofei Liu, Fan Yang, Tao Song, Anrong Zeng, Qi Wang, Zhong Sun, Jun Shen
Carbohydrate Polymers.2011; 85(2): 334. CrossRef
Trends and Perspectives of Biosensors for Food and Environmental Virology
Rakesh Yadav, Sadhana Dwivedi, Sandeep Kumar, Ashok Chaudhury
Food and Environmental Virology.2010; 2(2): 53. CrossRef
Nested real-time PCR for hepatitis A detection
Y. Hu, I. Arsov
Letters in Applied Microbiology.2009; 49(5): 615. CrossRef
Analytical Methods for Food and Environmental Viruses
Kirsten Mattison, Sabah Bidawid
Food and Environmental Virology.2009; 1(3-4): 107. CrossRef
Efficient capture of infectious H5 avian influenza virus utilizing magnetic beads coated with anionic polymer
Akikazu Sakudo, Kazuyoshi Ikuta
Biochemical and Biophysical Research Communications.2008; 377(1): 85. CrossRef

Effect of Saliva miltiorrhiza Bunge on Antimicrobial Activity and Resistant Gene Regulation against Methicillin-Resistant Staphylococcus aureus (MRSA): Ji-Won Lee , Young-Ju Ji , Syng-Ook Lee , In-Seon Lee; J. Microbiol. 2007;45(4):350-357.; DOI: https://doi.org/2561 [pii]

531 View
0 Download

Abstract PDF: This study was conducted in an effort to evaluate the antimicrobial activity and antibiotic-resistant gene regulation from Saliva miltiorrhiza Bunge on methicillin-resistant Staphylococcus aureus (MRSA). A variety of solvent fractions and methanol extracts of S. miltiorrhiza Bunge were tested in order to determine its antimicrobial activities against S. aureus and MRSA. As a result, the hexane fraction of S. miltiorrhiza Bunge evidenced the highest levels of antimicrobial activity against S. aureus and MRSA. The MICs of the hexane fraction against various MRSA specimens were 64<MICs≤128 μg/ml. The hexane fraction evidenced inhibitory effects superior to those of the chloroform fraction. The results showed inhibition zones of hexane (16 mm) and chloroform (14 mm) fractions against MRSA KCCM 40511 at 1,000 μg/disc. The hexane and chloroform fractions inhibited the expression of the resistant genes, mecA, mecR1, and femA in mRNA. Moreover, the results of Western blotting assays indicated that the hexane and chloroform fractions inhibited the expression of the resistant protein, PBP2a. These results reveal that the hexane and chloroform fractions of S. miltiorrhiza Bunge may prove to be a valuable choice for studies targeted toward the development of new antimicrobial agents.

Author index