- Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis
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Min-Ah Lee , Si-Mook Kang , Se-Yeon Kim , Ji-Soo Kim , Jin-Bom Kim , Seung-Hwa Jeong
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J. Microbiol. 2018;56(9):628-633. Published online August 23, 2018
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DOI: https://doi.org/10.1007/s12275-018-7515-7
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The aim of this study was to measure changes in the fluorescence
of Fusobacterium nucleatum interacting with Porphyromonas
gingivalis for excitation with blue light at 405-nm.
P. gingivalis was mono- and co-cultivated in close proximity
with F. nucleatum. The fluorescence of the bacterial colonies
was photographed using a QLF-D (Quantitative Light-induced
Fluorescence-Digital) Biluminator camera system with
a 405 nm light source and a specific filter. The red, green and
blue intensities of fluorescence images were analyzed using
the image analysis software. A fluorescence spectrometer was
used to detect porphyrin synthesized by each bacterium. F.
nucleatum, which emitted green fluorescence in single cultures,
showed intense red fluorescence when it was grown
in close proximity with P. gingivalis. F. nucleatum co-cultivated
with P. gingivalis showed the same pattern of fluorescence
peaks as for protoporphyrin IX in the red part of
the spectrum. We conclude that the green fluorescence of
F. nucleatum can change to red fluorescence in the presence
of adjacent co-cultured with P. gingivalis, indicating that
the fluorescence character of each bacterium might depend
on the presence of other bacteria.
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Citations
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