- Isolation of a novel Lactiplantibacillus plantarum strain resistant to nitrite stress and its transcriptome analysis
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Chae Young Kwon , Kyoung Jin Choi , Dongeun Yong , Ji-Eun Kim , Sang Sun Yoon
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J. Microbiol. 2022;60(7):715-726. Published online July 4, 2022
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DOI: https://doi.org/10.1007/s12275-022-2221-x
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Nitric oxide (NO) is a reactive nitrogen species (RNS) that
plays a vital role in regulating inflammatory processes. Under
abnormal conditions, excessive NO levels can promote the
oxidation of cellular components, which may cause or exacerbate
diseases such as hypertension, cardiovascular dysfunction,
and inflammatory bowel disease (IBD). Previous
studies have shown that reducing NO levels in the lumen can
attenuate the clinical symptoms of IBD. Thus, we aimed to
identify bacteria that can reduce RNS and that can be used
as valuable probiotics. In this study, we isolated bacteria resistant
to nitrite stress from human feces and used 16S and
whole-genome sequencing to identify them as Lactiplantibacillus
plantarum LP7 (LP7). The ability to survive at high
nitrite levels and to decrease them was greater in the LP7 strain
than in the reference strain L. plantarum ATCC14917 (ATCC-
14917). To characterize the LP7 genome in more detail, we
performed a comparative genome analysis. However, the unique
genes that directly confer the ability to withstand nitrite
stress were not present in the LP7 genome. Furthermore, we
performed transcriptomic analysis of LP7 and ATCC14917
cells treated with nitrite. We found that the expression levels
of genes involved in the cell division process were induced in
LP7, which showed a more regular rod-shape than ATCC-
14917. This could explain why LP7 can survive better than
ATCC14917 under nitrite stress. Based on its ability to survive
better in nitrite stress and decrease nitrite concentration,
we suggest that LP7 could be a valuable probiotic strain.
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Citations
Citations to this article as recorded by  - Nitrite stress acclimation of Pediococcus pentosaceus improves nitrite degradation in fermented sausages: from phenotype to transcriptomic mechanism
Meijun Zeng, Yanan Zhou, Xiaoping Yu, Zhe Bai, Lili Ji, Jiamin Zhang, Wei Wang
Meat Science.2026; 242: 110206. CrossRef - Transcriptomic insights into nitrite stress responses associated with efficient nitrite removal by Leuconostoc mesenteroides SIIA 9049 in fermented sausages
Meijun Zeng, Xiangyue Cui, Xiaohua Huang, Bo Wang, Shu Wang, Lili Ji, Jiamin Zhang, Wei Wang
Food Research International.2026; 245: 120854. CrossRef
- Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439
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Ji-Eun Kim , Joon-Sun Choi , Jung-Hye Roe
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J. Microbiol. 2019;57(5):388-395. Published online February 5, 2019
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DOI: https://doi.org/10.1007/s12275-019-8539-3
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1,058
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Abstract
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Streptomycetes naturally produce a variety of secondary
metabolites, in the process of physiological differentiation.
Streptomyces venezuelae differentiates into spores in liquid
media, serving as a good model system for differentiation and
a host for exogenous gene expression. Here, we report the
growth and differentiation properties of S. venezuelae ATCC-
15439 in liquid medium, which produces pikromycin, along
with genome-wide gene expression profile. Comparison of
growth properties on two media (SPA, MYM) revealed that
the stationary phase cell viability rapidly decreased in SPA.
Submerged spores showed partial resistance to lysozyme and
heat, similar to what has been observed for better-characterized
S. venezuelae ATCC10712, a chloramphenicol producer.
TEM revealed that the differentiated cells in the submerged
culture showed larger cell size, thinner cell wall than
the aerial spores. We analyzed transcriptome profiles of cells
grown in liquid MYM at various growth phases. During
transition and/or stationary phases, many differentiationrelated
genes were well expressed as judged by RNA level,
except some genes forming hydrophobic coats in aerial mycelium.
Since submerged spores showed thin cell wall and
partial resistance to stresses, we examined cellular expression
of MreB protein, an actin-like protein known to be required
for spore wall synthesis in Streptomycetes. In contrast to
aerial spores where MreB was localized in septa and spore
cell wall, submerged spores showed no detectable signal.
Therefore, even though the mreB transcripts are abundant in
liquid medium, its protein level and/or its interaction with
spore wall synthetic complex appear impaired, causing thinner-
walled and less sturdy spores in liquid culture.
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Citations
Citations to this article as recorded by - Colony politics to chemical warfare: phenotypic plasticity in Streptomyces
Freya Harrison, Rachel Wheatley, John T. Munnoch, Paul A. Hoskisson
Essays in Biochemistry.2026; 70(3): 399. CrossRef - Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712
Hung‐En Lai, Agata Kennedy, Lewis Tanner, Emma A. Bartram, Soo Mei Chee, Paul S. Freemont, Simon J. Moore
ChemBioChem.2024;[Epub] CrossRef - Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439
Shuai Li, Zhong Li, Guoqiang Zhang, Vlada B. Urlacher, Li Ma, Shengying Li
Engineering Microbiology.2024; 4(4): 100166. CrossRef - Glucose-1-phosphate thymidylyltransferase promotes the production of 3-O-α-mycarosylerythronolide B in Streptomyces coelicolor
Hong Gao, Swen Langer, Tony Larson, Matthew A Gregory, Margaret C M Smith
Journal of Applied Microbiology.2024;[Epub] CrossRef - Comparative Metagenomics Reveals Microbial Signatures of Sugarcane Phyllosphere in Organic Management
Ahmad Nuruddin Khoiri, Supapon Cheevadhanarak, Jiraporn Jirakkakul, Sudarat Dulsawat, Peerada Prommeenate, Anuwat Tachaleat, Kanthida Kusonmano, Songsak Wattanachaisaereekul, Sawannee Sutheeworapong
Frontiers in Microbiology.2021;[Epub] CrossRef - Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme
Ji-Eun Kim, Joon-Sun Choi, Jong-Seo Kim, You-Hee Cho, Jung-Hye Roe
Nucleic Acids Research.2020; 48(5): 2401. CrossRef - Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle Cerambyx welensii
Ramón I. Santamaría, Ana Martínez-Carrasco, Ricardo Sánchez de la Nieta, Luis M. Torres-Vila, Raúl Bonal, Jesús Martín, Rubén Tormo, Fernando Reyes, Olga Genilloud, Margarita Díaz
Microorganisms.2020; 8(12): 2013. CrossRef
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