- Comparative Proteome Analysis of Bacillus anthracis with pXO1 Plasmid Content
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Sudipto Shahid , Ji Hyun Park , Hyung Tae Lee , Seong-Joo Kim , Ji Cheon Kim , Sang Hoon Kim , Dal Mu Ri Han , Dong In Jeon , Kyoung Hwa Jung , Young Gyu Chai
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J. Microbiol. 2010;48(6):771-777. Published online January 9, 2011
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DOI: https://doi.org/10.1007/s12275-010-0136-4
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Bacillus anthracis the causative agent of anthrax, is an important pathogen among the Bacillus cereus group of species because of its physiological characteristics and its importance as a biological warfare agent. Tripartite anthrax toxin proteins and a poly-D-glutamic acid capsule are produced by B. anthracis vegetative
cells during mammalian hosts infection and when cultured in conditions that are thought to mimic the host environment. To identify the factors regulating virulence in B. anthracis the whole cell proteins were extracted from two B. anthracis strains and separated by narrow range immobilized pH gradient (IPG) strips (pH 4-7),
followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins that were differentially expressed were identified by the peptide fingerprinting using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS). A total of 23 proteins were identified as
being either upregulated or downregulated in the presence or absence of the virulence plasmid pXO1. Two plasmid encoded proteins and 12 cellular proteins were identified and documented as potential virulence factors.
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Citations
Citations to this article as recorded by  - Tracking Bacillus anthracis: The Legacy of MALDI-TOF Biomarkers in Scientific Literature, a Review
Jacqueline Roberta Salgado, Monique Cardozo, Samir F. A. Cavalcante, Adriana Marcos Vivoni, Ian Gardel Silva, Valdir F da Veiga-Junior
ACS Omega.2026; 11(1): 229. CrossRef - Identification and Validation of Specific Markers of Bacillus anthracis Spores by Proteomics and Genomics Approaches
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Molecular & Cellular Proteomics.2014; 13(3): 716. CrossRef - Genetic diversity of KoreanBacillus anthracisisolates from soil evaluated with a single nucleotide repeat analysis
Sang Hoon Kim, Kyoung Hwa Jung, Se Kye Kim, Seong-Joo Kim, Ji-Cheon Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Yun Ki Kim, Hyun Chul Hwang, Sam Gon Ryu, Young Gyu Chai
Journal of Veterinary Science.2013; 14(4): 457. CrossRef - Genetic populations ofBacillus anthracisisolates from Korea
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Journal of Veterinary Science.2012; 13(4): 385. CrossRef - Comparative analysis of virulence factors secreted by Bacillus anthracis Sterne at host body temperature
S.K. Kim, S. Shahid, S.H. Kim, J.H. Park, H.T. Lee, K.H. Jung, Y.G. Chai
Letters in Applied Microbiology.2012; 54(4): 306. CrossRef
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