- Functional Characterization of Extracellular Chitinase Encoded by the YlCTS1 Gene in a Dimorphic Yeast Yarrowia lipolytica
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Jeong-Nam Park , Chang Pyo Han , Dong-Jik Lee , Seon Ah Cheon , Hyun Ah Kang
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J. Microbiol. 2014;52(4):284-291. Published online March 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4070-8
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The hemiascomycetes yeast Yarrowia lipolytica is a dimorphic yeast with alternating yeast and mycelia forms. Bioinformatic analysis revealed the presence of three putative chitinase genes, YlCTS1, YlCTS2, and YlCTS3, in the Y. lipolytica genome. Here, we demonstrated that the protein of YlCTS1 (YlCts1p), which contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and
a chitin-binding domain, is a homologue to Saccharomyces cerevisiae chitinase 1 (ScCts1p). Deletion of YlCTS1 remarkably reduced extracellular endochitinase activity in the culture supernatant of Y. lipolytica and enhanced cell aggregation,
suggesting a role of YlCts1p in cell separation as ScCts1p does in S. cerevisiae. However, loss of YlCts1p function did not affect hyphal formation induced by fetal bovine serum addition. The mass of YlCts1p was dramatically decreased
by jack bean α-mannosidase digestion but not by PNGase F treatment, indicating that YlCts1p is modified only by Omannosylation without N-glycosylation. Moreover, the O-glycan profile of YlCts1p was identical to that of total cell wall
mannoproteins, supporting the notion that YlCts1p can be used as a good model for studying O-glycosylation in this dimorphic yeast.
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Citations
Citations to this article as recorded by  - Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing
Jonathan Harris, Michael Brothers, Victoria Coyle, Steve Kim, Erin Ratcliff
Chemistry of Materials.2024; 36(1): 324. CrossRef - The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein
Carmen-Lisset Flores, Joaquín Ariño, Carlos Gancedo
International Journal of Molecular Sciences.2021; 22(23): 13109. CrossRef - Recovery and valorization of agri-food wastes and by-products using the non-conventional yeast Yarrowia lipolytica
Davide Gottardi, Lorenzo Siroli, Lucia Vannini, Francesca Patrignani, Rosalba Lanciotti
Trends in Food Science & Technology.2021; 115: 74. CrossRef - Functional analysis of recombinant human and Yarrowia lipolytica O-GlcNAc transferases expressed in Saccharomyces cerevisiae
Hye Ji Oh, Hye Yun Moon, Seon Ah Cheon, Yoonsoo Hahn, Hyun Ah Kang
Journal of Microbiology.2016; 54(10): 667. CrossRef
- Functional Analysis of a Hansenula polymorpha MNN2-2 Homologue Encoding a Putative UDP-N-acetylglucosamine Transporter Localized in the Endoplasmic Reticulum
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Jeong-Nam Park , Jinho Choo , Hyun Ah Kang
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J. Microbiol. 2011;49(6):1012-1017. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1520-4
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The Kluyveromyces lactis UDP-GlcNAc transporter (KlMnn2-2p) is responsible for the biosynthesis of N-glycans containing N-acetylglucosamine. A putative gene of Hansenula polymorpha encoding a KlMnn2-2p homologue, HpMNN2-2, was identified and investigated for its function. The deletion mutant strain of HpMNN2-2 (Hpmnn2-2Δ) showed increased sensitivity to geneticin, hygromycin B, and tunicamycin. However, the Hpmnn2-2Δ strain exhibited increased resistance to Calcofluor white, an inhibitor of chitin biosynthesis, along with a reduced chitin content. The localization of HpMnn2-2p at the endoplasmic reticulum-enriched membrane, different from the Golgi localization of a K. lactis homologue, further supports the involvement of HpMnn2-2p in cell wall chitin biosynthesis.
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Citations
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Yizhou Gao, Xiaohui Xiong, Hui Wang, Jiajing Wang, Yan Bi, Yuqing Yan, Zhongye Cao, Dayong Li, Fengming Song
Environmental Microbiology.2022; 24(3): 1200. CrossRef - Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production
João Heitor Colombelli Manfrão-Netto, Enzo Bento Queiroz, Kelly Assis Rodrigues, Cintia M. Coelho, Hugo Costa Paes, Elibio Leopoldo Rech, Nádia Skorupa Parachin
Microorganisms.2021; 9(2): 312. CrossRef - Nucleotide sugar transporters of Entamoeba histolytica and Entamoeba invadens involved in chitin synthesis
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Molecular and Biochemical Parasitology.2019; 234: 111224. CrossRef - Remodeling of the glycosylation pathway in the methylotrophic yeast Hansenula polymorpha to produce human hybrid-type N-glycans
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The Journal of Microbiology.2012; 50(2): 341. CrossRef
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