- Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay
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Rungkarn Suebsing , Jeong-Ho Kim , Seok Ryel Kim , Myung-Ae Park , Myung-Joo Oh
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J. Microbiol. 2011;49(5):741-746. Published online November 9, 2011
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DOI: https://doi.org/10.1007/s12275-011-1209-8
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The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in farmed rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.
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Citations
Citations to this article as recorded by  - The development and initial evaluation of a multiplex RT-PCR and high-throughput liquid chip assay for the simultaneous detection of five salmonid virus diseases
Yu Chen, Shuai Gao, Hao Xu, Songqi Zhu, Guixiang Tong, Xiaoyu Chen, Xinxian Wei, Hong Liu, Xiaocong Zheng
Aquaculture Reports.2024; 36: 102163. CrossRef - Comprehensive review on infectious pancreatic necrosis virus
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Journal of Semiconductors.2023; 44(2): 023103. CrossRef - Piscine orthoreovirus: Biology and distribution in farmed and wild fish
Mark P. Polinski, Niccoló Vendramin, Argelia Cuenca, Kyle A. Garver
Journal of Fish Diseases.2020; 43(11): 1331. CrossRef - Infectious hematopoietic necrosis virus: advances in diagnosis and vaccine development
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Gouranga Biswas, Masahiro Sakai
Applied Microbiology and Biotechnology.2014; 98(7): 2881. CrossRef - A one‐step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain
W. W. Zeng, Q. Wang, Y. Y. Wang, D. H. Xu, S. Q. Wu
Journal of Fish Biology.2013; 82(5): 1545. CrossRef
- Detection of Marine Birnavirus (MBV) from Rockfish Sebastes schlegeli Using Reverse Transcription and Nested PCR
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Seong-Joon Joh , Doo-Won Kim , Jeong-Ho Kim , Gang-Joon Heo
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J. Microbiol. 2000;38(4):260-264.
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Reverse transcription (RT)-PCR and nested PCR methods (2-step PCR) were tested for their ability to detect marine birnavirus (MBV) in cultured rockfish, Sebastes schlegeli. One set of primers for RT-PCR was designed, based on a gene of infectious pancreatic necrosis virus (IPNV), and another set of primers for nested PCR was designed based on the VP2/NS junction region of MBV. This 2-step PCR method was specific for MBV and sensitivity was heightened when nested PCR was combined to RT-PCR. This 2-step PCR method was useful for detecting MBV not only in diseased fish, but also in asymptomatic fish. These results indicate that this 2-step PCR method is useful for detecting MBV in rockfish.
- Genetic Analysis of the VP2/NS Junction Region on Segment A of Marine Birnavirus Isolated from Cultured Flounder Paralichthys olivaceous
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Seong-Joon Joh , Jeong-Ho Kim , Gang-Joon Heo
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J. Microbiol. 2000;38(1):44-47.
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The cDNA of VP2/NS junction region on segment A of the marine birnavirus (MBV) isolated from flounder (DS strain) was amplified using the reverse transcription (RT)-polymerase chain reaction (PCR). Its cDNA nucleotide and deduced amino acid sequences were analyzed, and compared with the reference strains of MBV and infectious pancreatic necrosis virus (IPNV). Analyses of the nucleotide and deduced amino acid sequences revealed that the DS strain is very similar to the reference strains of MBV, distant from the IPNV.
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