- Diversity of Yeasts Associated with Panax ginseng
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Soon Gyu Hong , Kang Hyun Lee , Jangyul Kwak , Kyung Sook Bae
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J. Microbiol. 2006;44(6):674-679.
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DOI: https://doi.org/2457 [pii]
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 Abstract
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Biodiversity of yeasts was investigated in the ginseng cultivation field. Among 34 isolates tested in this study, 26 isolates belonged to the hymenomycetous yeast group. These 26 strains were classified into 12 species including four new-species candidates that did not have clear affiliation to any established species. Seven isolates among the remaining strains were classified into three ascomycetous yeast species, and one isolate was identified as a urediniomycetous yeast species.
- Sequence Analysis and Functional Expression of the Structural and Regulatory Genes for Pyruvate Dehydrogenase Complex from Streptomyces seoulensis
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Hwan Youn , Jangyul Kwak , Dong-Won Kim , Chang-Jin Lee , Yang-In Yim , Jin-Won Lee , In-Kwon Kim , Jeong-Il Yu , Hyung-Soon Yim , Sa-Ouk Kang
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J. Microbiol. 2002;40(1):43-50.
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Abstract
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A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (E1p). E1p had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria. S. seoulensis E2p contains two lipoyl domains like those of humans and Streptococcus faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis showed growth retardation and the decrease of E1p, indicating that PdhR regulates the function of PDC by repressing the expression of E1p. A strain of Streptomyces lividans overexpressing S. seoulensis PdhR showed a significant decrease in the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.
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