- Resistance of Saccharomyces cerevisiae to Fungicide Chlorothalonil
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Jae-Ho Shin , Young-Mog Kim , Jong-Woo Park , Jang-Eok Kim , In-Koo Rhee
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J. Microbiol. 2003;41(3):219-223.
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Abstract
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The toxicity of chlorothalonil on the growth of yeasts was investigated using several yeast strains. An alcohol tolerant yeast, Saccharomyces cerevisiae F38-1, was the most chlorothalonil-tolerant. The glutathione content and the glutathione S-transferase activity were related to the chlorothalonil-tolerant phenotype. Several thiol compounds affect the dissipation of chlorothalonil. However, there was no significant difference on the effects of chlorothalonil dissipation among the thiol compounds tested. The growth of yeast cells was arrested by chlorothalonil. It took about 13 h to dissipate 1 mg/l of chlorothalonil, and the growth was restored as the chlorothalonil content decreased. The glutathione content and glutathione S-transferase are suggested to be among the most important factors of yeast resistance to chlorothalonil.
- Isolation and Identification of a Pentachloronitrobenzene (PCNB) Degrading Bacterium Alcaligenes xylosoxidans PCNB-2 from Agricultural Soil
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Sung-Kyu Shin , Jang-Eok Kim , Gi-Seok Kwon , Jin-Wook Kwon , Eun-Taex Oh , Jae-Seong So , Sung-Cheol Koh
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J. Microbiol. 2003;41(2):165-168.
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Abstract
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We report a new PCNB-degrading strain (PCNB-2) that is able to utilize and grow on PCNB (100 ppm) as a sole carbon source. This strain was identified as Alcaligenes xylosoxidans based upon 16S rDNA sequence analysis, API 20 NE tests and cell membrane lipid analysis. The new PCNB degrader Alcali-genes xylosoxidans PCNB-2 could find use in bioremediation of PCNB, which is environmentally persistent.
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