Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Browse Articles > Author index
Search
Jaewon Ryu 2 Articles
Microbial consortia including methanotrophs: some benefits of living together
Rajendra Singh , Jaewon Ryu , Si Wouk Kim
J. Microbiol. 2019;57(11):939-952.   Published online October 28, 2019
DOI: https://doi.org/10.1007/s12275-019-9328-8
  • 1,052 View
  • 4 Download
  • 39 Web of Science
  • 40 Crossref
AbstractAbstract PDF
With the progress of biotechnological research and improvements made in bioprocessing with pure cultures, microbial consortia have gained recognition for accomplishing biological processes with improved effectiveness. Microbes are indispensable tool in developing bioprocesses for the production of bioenergy and biochemicals while utilizing renewable resources due to technical, economic and environmental advantages. They communicate with specific cohorts in close proximity to promote metabolic cooperation. Use of positive microbial associations has been recognized widely, especially in food industries and bioremediation of toxic compounds and waste materials. Role of microbial associations in developing sustainable energy sources and substitutes for conventional fuels is highly promising with many commercial prospects. Detoxification of chemical contaminants sourced from domestic, agricultural and industrial wastes has also been achieved through microbial catalysis in pure and co-culture systems. Methanotrophs, the sole biological sink of greenhouse gas methane, catalyze the methane monooxygenasemediated oxidation of methane to methanol, a high energy density liquid and key platform chemical to produce commodity chemical compounds and their derivatives. Constructed microbial consortia have positive effects, such as improved biomass, biocatalytic potential, stability etc. In a methanotroph- heterotroph consortium, non-methanotrophs provide key nutrient factors and alleviate the toxicity from the culture. Non-methanotrophic organisms biologically stimulate the growth and activity of methanotrophs via production of growth stimulators. However, methanotrophs in association with cocultured microorganisms are in need of further exploration and thorough investigation to study their interaction mode and application with improved effectiveness.

Citations

Citations to this article as recorded by  
  • Enrichment Culture of Methane-Oxidizing Bacteria and Acclimatization Experiment Research under Coal Mine Environmental Stress Mechanisms
    Jun Xie, Qian Yang, Dawei Chen, Yi Wang, Haigang Du
    Journal of Environmental Engineering.2026;[Epub]     CrossRef
  • Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
    Andrés Felipe Torres-Franco, Raquel Herrero-Lobo, Raquel Lebrero, Raúl Muñoz
    Bioresource Technology.2026; 444: 133920.     CrossRef
  • Harnessing Microbial Consortia for Sustainable Agriculture: Abiotic and Biotic Stress Mitigation
    Lobna Hajji-Hedfi, Takwa Wannassi, Amira Khlif, Omaima Bargougui, Samar Dali, Mariana Carmen Chifiriuc, Ahmed M. Abdel-Azeem
    Current Microbiology.2026;[Epub]     CrossRef
  • Development of a synergistic tripartite consortium and hybrid growth media for enhanced methane oxidation and assessment of in vitro plant growth-promoting traits
    C S Shivaranjan, Shivani Kaushik, Pushpendra Sharma, Arti Bhatia, Radha Prasanna, Rajeev Kaushik
    World Journal of Microbiology and Biotechnology.2026;[Epub]     CrossRef
  • Plant growth‐ promoting bacteria inoculation and co‐inoculation effects on Palisade grass (Urochloa brizantha cv. Marandu) in ferralsol in Amazonas State, Brazil
    Sara Rodrigues Batista, Cláudia Majolo, Rogério Perin, Marco Antonio Nogueira, Mariangela Hungria, Christiane Abreu Oliveira‐Paiva, Enílson Luíz Saccol de Sá, Christoph Müller, Maria Teresa Gomes Lopes, Aleksander Westphal Muniz
    Soil Science Society of America Journal.2026;[Epub]     CrossRef
  • Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1
    Thi Duc Thai, Jun Ren, So Hee Oh, Dokyun Na
    Journal of Biological Engineering.2026;[Epub]     CrossRef
  • Microbial amidases: Characterization, advances and biotechnological applications
    Rajendra Singh, Refana Shahul, Vijay Kumar, Ashok Kumar Yadav, Praveen Kumar Mehta
    Biotechnology Notes.2025; 6: 44.     CrossRef
  • Integrated bioprocess for single-cell protein production: Converting waste-derived biogas into sustainable feed protein
    Hafiz Muhammad Aamir Shahzad, Zukhruf Asim, Zainab I. Elkahlout, Khaled A. Mahmoud, Fares Almomani, Kashif Rasool
    Energy Conversion and Management: X.2025; 26: 101036.     CrossRef
  • A machine-learning approach for predicting butyrate production by microbial consortia using metabolic network information
    Claudia Silva-Andrade, Sergio Hernández, Pedro Saa, Ernesto Perez-Rueda, Daniel Garrido, Alberto J. Martin
    PeerJ.2025; 13: e19296.     CrossRef
  • Stable microbial community promotes aerobic methanotrophy in a large river-reservoir system
    Qiong Tang, Zhe Li, Lunhui Lu, Yan Xiao, Xinghua Wu, Dianchang Wang, Marcela Hernandez
    mSystems.2025;[Epub]     CrossRef
  • The Art of Amalgamation: Advancing Crop Productivity with PGPR Consortia Solutions
    Nishra Joshi, Bhargav Raval, Chaitanya Kumar Jha
    Current Agriculture Research Journal.2025; 13(1): 04.     CrossRef
  • Recent breakthrough in methanotrophy: Promising applications and future challenges
    Grishma A. Dave, Bhumi M. Javia, Suhas J. Vyas, Ramesh K. Kothari, Rajesh K. Patel, Dushyant R. Dudhagara
    Journal of Environmental Chemical Engineering.2025; 13(2): 115371.     CrossRef
  • Simultaneous co-cultivation of the thermoacidophilic methanotroph, Methylacidiphilum sp. RTK17.1, and the microalga, Galdieria sp. RTK37.1, for single cell protein production
    Carlos Cartin-Caballero, Christophe Collet, Daniel Gapes, Peter A. Gostomski, Matthew B. Stott, Carlo R. Carere
    Engineering Microbiology.2025; 5(4): 100229.     CrossRef
  • Methanol excretion by Methylomonas methanica is induced by the supernatant of a methanotrophic consortium
    Geovanni Avila‐Nuñez, Alexis Saldivar, Patricia Ruiz‐Ruiz, Sergio Revah
    Journal of Chemical Technology & Biotechnology.2024; 99(6): 1356.     CrossRef
  • Biorreducción in vitro de cromo hexavalente utilizando consorcios microbianos
    Stefany Serna-Toro, Fabiana María Lora-Suarez, Nelsy Loango-Chamorro
    Actualidades biológicas.2024;[Epub]     CrossRef
  • Using metabolic networks to predict cross-feeding and competition interactions between microorganisms
    Claudia Silva-Andrade, María Rodriguez-Fernández, Daniel Garrido, Alberto J. M. Martin, Paul A. Jensen
    Microbiology Spectrum.2024;[Epub]     CrossRef
  • Impacts of Soil Compaction and Phosphorus Levels on the Dynamics of Phosphate-Solubilizing and Nitrogen-Fixing Bacteria in the Peanut Rhizosphere
    Qi Wu, Liyu Yang, Haiyan Liang, Miao Liu, Yinglong Chen, Dianxu Chen, Pu Shen
    Agronomy.2024; 14(9): 1971.     CrossRef
  • Responses of composition and metabolism of microbial communities during the remediation of black and odorous water using bioaugmentation and aeration
    Dan Zhang, Yu Lei, Chen Wang, Shuhuan Lan, Xudong Li, Yifei Xie
    Environmental Research.2024; 243: 117895.     CrossRef
  • Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform
    Yu Liu, Boyuan Xue, Hao Liu, Shaojie Wang, Haijia Su
    Biotechnology Advances.2024; 72: 108348.     CrossRef
  • Characterizing A21: Natural Cyanobacteria-Based Consortium with Potential for Steroid Bioremediation in Wastewater Treatment
    Govinda Guevara, Jamileth Stefania Espinoza Solorzano, Marta Vargas Ramírez, Andrada Rusu, Juana María Navarro Llorens
    International Journal of Molecular Sciences.2024; 25(23): 13018.     CrossRef
  • Differential effects of rhizobacteria from uninfected and infected tomato on Meloidogyne incognita under protected cultivation
    Manjunatha T. Gowda, Radha Prasanna, Aditi Kundu, Virendra Singh Rana, Uma Rao, Gautam Chawla
    Journal of Basic Microbiology.2023; 63(6): 604.     CrossRef
  • Characteristics of the methanotroph used in coalbed methane emission reduction: Methane oxidation efficiency and coal wettability
    Yinbo Zhou, Ruilin Zhang, Kunyun Tian, Shuang Zhao, Hanfeng Shi, Weidong Gong, Qing Lei
    Fuel.2023; 349: 128596.     CrossRef
  • Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies
    Weronika Goraj, Anna Pytlak, Jarosław Grządziel, Anna Gałązka, Zofia Stępniewska, Anna Szafranek-Nakonieczna
    Biology.2023; 12(11): 1420.     CrossRef
  • Construction of synthetic anti-fouling consortia: fouling control effects and polysaccharide degradation mechanisms
    Ji Qi, Qicheng Zhou, Danlei Huang, Zhong Yu, Fangang Meng
    Microbial Cell Factories.2023;[Epub]     CrossRef
  • Enzymatic biomethanol production: Future perspective
    Saif Khan, Gourav Jain, Alka Srivastava, Praveen C. Verma, Veena Pande, Rama S. Dubey, Mahvish Khan, Shafiul Haque, Saheem Ahmad
    Sustainable Materials and Technologies.2023; 38: e00729.     CrossRef
  • Isolation of Methane Enriched Bacterial Communities and Application as Wheat Biofertilizer under Drought Conditions: An Environmental Contribution
    Adoración Barros-Rodríguez, Carlos García-Gálvez, Pamela Pacheco, Marina G. Kalyuzhnaya, Maximino Manzanera
    Plants.2023; 12(13): 2487.     CrossRef
  • An Overview on Methanotrophs and the Role of Methylosinus trichosporium OB3b for Biotechnological Applications
    Rajendra Singh, Jaewon Ryu, Si Wouk Kim
    Biotechnology and Bioprocess Engineering.2022; 27(4): 468.     CrossRef
  • Methanol Production using Free and Immobilized Cells of a Newly Isolated Methylosinus trichosporium M19-4
    Rajendra Singh, Jaewon Ryu, Si Wouk Kim
    KSBB Journal.2022; 37(3): 93.     CrossRef
  • Prospecting the significance of methane-utilizing bacteria in agriculture
    Vijaya Rani, Radha Prasanna, Rajeev Kaushik
    World Journal of Microbiology and Biotechnology.2022;[Epub]     CrossRef
  • The Effect of In Vitro Coinoculation on the Physiological Parameters of White Lupine Plants (Lupinus albus L.)
    Zyta Waraczewska, Alicja Niewiadomska, Agnieszka Wolna-Maruwka, Hanna Sulewska, Anna Budka, Agnieszka A. Pilarska
    Applied Sciences.2022; 12(23): 12382.     CrossRef
  • Genomic, biochemical, and phylogenetic evaluation of bacteria isolated from deep-sea sediment harboring methane hydrates
    Audrey Menegaz Proenca, Maiara Monteiro Oliveira, Paula Fernanda Ribas Neves, Adriana Giongo, Rafael Rodrigues de Oliveira, Carolina Telles Ott, Letícia Marconatto, Halesio Milton Correa de Barros Neto, João Marcelo Medina Ketzer, Renata Medina-Silva
    Archives of Microbiology.2022;[Epub]     CrossRef
  • An Overview of Microbial α-amylase and Recent Biotechnological Developments
    Rajendra Singh, Si Wouk Kim, Anila Kumari, Praveen Kumar Mehta
    Current Biotechnology.2022; 11(1): 11.     CrossRef
  • Enrichment of Methylosinus-dominant consortia from mangroves for polyhydroxybutyrate (PHB) production
    Letícia Oliveira Bispo Cardoso, Bruno Karolski, Louise Hase Gracioso, Bruna Bacaro Borrego, Cláudio Augusto Oller do Nascimento, Elen Aquino Perpetuo
    Journal of Environmental Chemical Engineering.2022; 10(5): 108490.     CrossRef
  • Functional Investigation of Plant Growth Promoting Rhizobacterial Communities in Sugarcane
    Mingjia Li, Ran Liu, Yanjun Li, Cunhu Wang, Wenjing Ma, Lei Zheng, Kefei Zhang, Xing Fu, Xinxin Li, Yachun Su, Guoqiang Huang, Yongjia Zhong, Hong Liao
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Conversion of methane to methanol: technologies and future challenges
    Rajesh K. Srivastava, Prakash Kumar Sarangi, Latika Bhatia, Akhilesh Kumar Singh, Krushna Prasad Shadangi
    Biomass Conversion and Biorefinery.2022; 12(5): 1851.     CrossRef
  • Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O2-Availability
    Amber Yasemin Shirin de Zoete, Hendrik Gideon Brink, Joshua Cornelus Beukes, Ignatius Leopoldus van Rooyen, Willie Nicol
    Processes.2021; 9(11): 2039.     CrossRef
  • Plant Growth Stimulation by Microbial Consortia
    Gustavo Santoyo, Paulina Guzmán-Guzmán, Fannie Isela Parra-Cota, Sergio de los Santos-Villalobos, Ma. del Carmen Orozco-Mosqueda, Bernard R. Glick
    Agronomy.2021; 11(2): 219.     CrossRef
  • Recent trends in methane to bioproduct conversion by methanotrophs
    Aleksandra Gęsicka, Piotr Oleskowicz-Popiel, Mateusz Łężyk
    Biotechnology Advances.2021; 53: 107861.     CrossRef
  • Modelling and multi-objective optimization for simulation of hydrogen production using a photosynthetic consortium
    Dulce J. Hernández-Melchor, Beni Camacho-Pérez, Elvira Ríos-Leal, Jesus Alarcón-Bonilla, Pablo A. López-Pérez
    International Journal of Chemical Reactor Engineering.2020;[Epub]     CrossRef
  • Environmental and Microbial Interactions Shape Methane-Oxidizing Bacterial Communities in a Stratified Lake
    Carole Guggenheim, Remo Freimann, Magdalena J. Mayr, Karin Beck, Bernhard Wehrli, Helmut Bürgmann
    Frontiers in Microbiology.2020;[Epub]     CrossRef
Enhanced method for microbial community DNA extraction and purification from agricultural yellow loess soil
Mathur Nadarajan Kathiravan , Geun Ho Gim , Jaewon Ryu , Pyung Il Kim , Chul Won Lee , Si Wouk Kim
J. Microbiol. 2015;53(11):767-775.   Published online October 28, 2015
DOI: https://doi.org/10.1007/s12275-015-5454-0
  • 980 View
  • 1 Download
  • 12 Crossref
AbstractAbstract PDF
In this study, novel DNA extraction and purification methods were developed to obtain high-quantity and reliable quality DNA from the microbial community of agricultural yellow loess soil samples. The efficiencies of five different soil DNAextraction protocols were evaluated on the basis of DNA yield, quality and DNA shearing. Our suggested extraction
method
, which used CTAB, EDTA and cell membrane lytic enzymes in the extraction followed by DNA precipitation using isopropanol, yielded a maximum DNA content of 42.28 ± 5.59 μg/g soil. In addition, among the five different purification protocols, the acid-treated polyvinyl polypyrrolidone (PVPP) spin column purification method yielded high-quality DNA and recovered 91% of DNA from the crude DNA. Spectrophotometry revealed that the ultraviolet A260/A230 and A260/A280 absorbance ratios of the purified DNA were 1.82 ± 0.03 and 1.94 ± 0.05, respectively. PCR-based 16S rRNA amplification showed clear bands at ~1.5 kb with acid-treated PVPP–purified DNA templates. In conclusion, our suggested extraction and purification protocols can be used to recover high concentration, high purity, and high-molecular-weight DNA from clay and silica-rich agricultural soil samples.

Citations

Citations to this article as recorded by  
  • DNA of the three elements: development of molecular genetic techniques in environmental research
    Svetlana Galkina
    Priroda.2025; (2(1314)): 3.     CrossRef
  • Inhibitory Effect of Volatiles from Flavobacterium johnsoniae TR-18 on Aspergillus flavus Growth and Aflatoxin Production
    Andong Gong, Jingrong Liu, Gaozhan Wang, Mengge Song, Jianhua Wang, Jingbo Zhang
    Plant Disease.2025; 109(2): 471.     CrossRef
  • Better Performance of Organic Fertilizer on Improving Yield and Reducing Nitrogen Losses in a Paddy Field as Compared to Biochar-Based Fertilizer
    Ke Wang, Shanshan Ying
    Water, Air, & Soil Pollution.2025;[Epub]     CrossRef
  • TACKLING THE SOIL MICROBIOME – CHALLENGES AND OPORTUNITIES
    Andreea-Mihaela Mlesnita
    Journal of Experimental and Molecular Biology.2024;[Epub]     CrossRef
  • Effects of sodium sulfide application on the growth of Robinia pseudoacacia, heavy metal immobilization, and soil microbial activity in Pb–Zn polluted soil
    Xiangyu Zhang, Xiao Lou, Haoqiang Zhang, Wei Ren, Ming Tang
    Ecotoxicology and Environmental Safety.2020; 197: 110563.     CrossRef
  • Biases from different DNA extraction methods in intestine microbiome research based on 16S rDNA sequencing: a case in the koi carp, Cyprinus carpio var. Koi
    Zhuoran Han, Jingfeng Sun, Aijun Lv, Anli Wang
    MicrobiologyOpen.2019;[Epub]     CrossRef
  • Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    Laynet Angerlyn Puentes , Yusibeska Ramos, Ysvic Inojosa, César Rivera, Angela De Sisto
    BIO-PROTOCOL.2019;[Epub]     CrossRef
  • Impact of DNA extraction methods on the observed microbial communities from the intestinal flora of the penaeid shrimp Litopenaeus vannamei
    Boyun Jiang, Jingfeng Sun, Aijun Lv, Xiucai Hu, Hongyue Shi, YeongYik Sung, Qingkui Wang, Yang Wang
    FEMS Microbiology Letters.2019;[Epub]     CrossRef
  • Illumina sequencing and assessment of new cost-efficient protocol for metagenomic-DNA extraction from environmental water samples
    Mariam Hassan, Tamer Essam, Salwa Megahed
    Brazilian Journal of Microbiology.2018; 49: 1.     CrossRef
  • A modified method for genomic DNA extraction from the fish intestinal microflora
    Zhuoran Han, Jingfeng Sun, Aijun Lv, YeongYik Sung, Xueliang Sun, Hongyue Shi, Xiucai Hu, Anli Wang, Kezhi Xing
    AMB Express.2018;[Epub]     CrossRef
  • Isolation of nematode DNA from 100 g of soil using Fe3O4 super paramagnetic nanoparticles
    Adrienne M. Gorny, Frank S. Hay, Xiaohong Wang, Sarah J. Pethybridge
    Nematology.2018; 20(3): 271.     CrossRef
  • Evaluation of the Punch-it™ NA-Sample kit for detecting microbial DNA in blood culture bottles using PCR-reverse blot hybridization assay
    Jungho Kim, Hye-young Wang, Seoyong Kim, Soon Deok Park, Kwangmin Yu, Hyo Youl Kim, Young Uh, Hyeyoung Lee
    Journal of Microbiological Methods.2016; 128: 24.     CrossRef

Journal of Microbiology : Journal of Microbiology
TOP