- Microbial consortia including methanotrophs: some benefits of living together
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Rajendra Singh , Jaewon Ryu , Si Wouk Kim
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J. Microbiol. 2019;57(11):939-952. Published online October 28, 2019
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DOI: https://doi.org/10.1007/s12275-019-9328-8
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With the progress of biotechnological research and improvements
made in bioprocessing with pure cultures, microbial
consortia have gained recognition for accomplishing biological
processes with improved effectiveness. Microbes are
indispensable tool in developing bioprocesses for the production
of bioenergy and biochemicals while utilizing renewable
resources due to technical, economic and environmental
advantages. They communicate with specific cohorts
in close proximity to promote metabolic cooperation. Use of
positive microbial associations has been recognized widely,
especially in food industries and bioremediation of toxic compounds
and waste materials. Role of microbial associations
in developing sustainable energy sources and substitutes for
conventional fuels is highly promising with many commercial
prospects. Detoxification of chemical contaminants sourced
from domestic, agricultural and industrial wastes has also been
achieved through microbial catalysis in pure and co-culture
systems. Methanotrophs, the sole biological sink of greenhouse
gas methane, catalyze the methane monooxygenasemediated
oxidation of methane to methanol, a high energy
density liquid and key platform chemical to produce commodity
chemical compounds and their derivatives. Constructed
microbial consortia have positive effects, such as improved
biomass, biocatalytic potential, stability etc. In a methanotroph-
heterotroph consortium, non-methanotrophs provide
key nutrient factors and alleviate the toxicity from the culture.
Non-methanotrophic organisms biologically stimulate the
growth and activity of methanotrophs via production of growth
stimulators. However, methanotrophs in association with cocultured
microorganisms are in need of further exploration
and thorough investigation to study their interaction mode
and application with improved effectiveness.
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Citations
Citations to this article as recorded by 
- Enrichment Culture of Methane-Oxidizing Bacteria and Acclimatization Experiment Research under Coal Mine Environmental Stress Mechanisms
Jun Xie, Qian Yang, Dawei Chen, Yi Wang, Haigang Du Journal of Environmental Engineering.2026;[Epub] CrossRef - Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
Andrés Felipe Torres-Franco, Raquel Herrero-Lobo, Raquel Lebrero, Raúl Muñoz Bioresource Technology.2026; 444: 133920. CrossRef - Harnessing Microbial Consortia for Sustainable Agriculture: Abiotic and Biotic Stress Mitigation
Lobna Hajji-Hedfi, Takwa Wannassi, Amira Khlif, Omaima Bargougui, Samar Dali, Mariana Carmen Chifiriuc, Ahmed M. Abdel-Azeem Current Microbiology.2026;[Epub] CrossRef - Development of a synergistic tripartite consortium and hybrid growth media for enhanced methane oxidation and assessment of in vitro plant growth-promoting traits
C S Shivaranjan, Shivani Kaushik, Pushpendra Sharma, Arti Bhatia, Radha Prasanna, Rajeev Kaushik World Journal of Microbiology and Biotechnology.2026;[Epub] CrossRef - Plant growth‐ promoting bacteria inoculation and co‐inoculation effects on Palisade grass (Urochloa brizantha cv. Marandu) in ferralsol in Amazonas State, Brazil
Sara Rodrigues Batista, Cláudia Majolo, Rogério Perin, Marco Antonio Nogueira, Mariangela Hungria, Christiane Abreu Oliveira‐Paiva, Enílson Luíz Saccol de Sá, Christoph Müller, Maria Teresa Gomes Lopes, Aleksander Westphal Muniz Soil Science Society of America Journal.2026;[Epub] CrossRef - Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1
Thi Duc Thai, Jun Ren, So Hee Oh, Dokyun Na Journal of Biological Engineering.2026;[Epub] CrossRef - Microbial amidases: Characterization, advances and biotechnological applications
Rajendra Singh, Refana Shahul, Vijay Kumar, Ashok Kumar Yadav, Praveen Kumar Mehta Biotechnology Notes.2025; 6: 44. CrossRef - Integrated bioprocess for single-cell protein production: Converting waste-derived biogas into sustainable feed protein
Hafiz Muhammad Aamir Shahzad, Zukhruf Asim, Zainab I. Elkahlout, Khaled A. Mahmoud, Fares Almomani, Kashif Rasool Energy Conversion and Management: X.2025; 26: 101036. CrossRef - A machine-learning approach for predicting butyrate production by microbial consortia using metabolic network information
Claudia Silva-Andrade, Sergio Hernández, Pedro Saa, Ernesto Perez-Rueda, Daniel Garrido, Alberto J. Martin PeerJ.2025; 13: e19296. CrossRef - Stable microbial community promotes aerobic methanotrophy in a large river-reservoir system
Qiong Tang, Zhe Li, Lunhui Lu, Yan Xiao, Xinghua Wu, Dianchang Wang, Marcela Hernandez mSystems.2025;[Epub] CrossRef - The Art of Amalgamation: Advancing Crop Productivity with PGPR Consortia Solutions
Nishra Joshi, Bhargav Raval, Chaitanya Kumar Jha Current Agriculture Research Journal.2025; 13(1): 04. CrossRef - Recent breakthrough in methanotrophy: Promising applications and future challenges
Grishma A. Dave, Bhumi M. Javia, Suhas J. Vyas, Ramesh K. Kothari, Rajesh K. Patel, Dushyant R. Dudhagara Journal of Environmental Chemical Engineering.2025; 13(2): 115371. CrossRef - Simultaneous co-cultivation of the thermoacidophilic methanotroph, Methylacidiphilum sp. RTK17.1, and the microalga, Galdieria sp. RTK37.1, for single cell protein production
Carlos Cartin-Caballero, Christophe Collet, Daniel Gapes, Peter A. Gostomski, Matthew B. Stott, Carlo R. Carere Engineering Microbiology.2025; 5(4): 100229. CrossRef - Methanol excretion by Methylomonas methanica is induced by the supernatant of a methanotrophic consortium
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Stefany Serna-Toro, Fabiana María Lora-Suarez, Nelsy Loango-Chamorro Actualidades biológicas.2024;[Epub] CrossRef - Using metabolic networks to predict cross-feeding and competition interactions between microorganisms
Claudia Silva-Andrade, María Rodriguez-Fernández, Daniel Garrido, Alberto J. M. Martin, Paul A. Jensen Microbiology Spectrum.2024;[Epub] CrossRef - Impacts of Soil Compaction and Phosphorus Levels on the Dynamics of Phosphate-Solubilizing and Nitrogen-Fixing Bacteria in the Peanut Rhizosphere
Qi Wu, Liyu Yang, Haiyan Liang, Miao Liu, Yinglong Chen, Dianxu Chen, Pu Shen Agronomy.2024; 14(9): 1971. CrossRef - Responses of composition and metabolism of microbial communities during the remediation of black and odorous water using bioaugmentation and aeration
Dan Zhang, Yu Lei, Chen Wang, Shuhuan Lan, Xudong Li, Yifei Xie Environmental Research.2024; 243: 117895. CrossRef - Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform
Yu Liu, Boyuan Xue, Hao Liu, Shaojie Wang, Haijia Su Biotechnology Advances.2024; 72: 108348. CrossRef - Characterizing A21: Natural Cyanobacteria-Based Consortium with Potential for Steroid Bioremediation in Wastewater Treatment
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Manjunatha T. Gowda, Radha Prasanna, Aditi Kundu, Virendra Singh Rana, Uma Rao, Gautam Chawla Journal of Basic Microbiology.2023; 63(6): 604. CrossRef - Characteristics of the methanotroph used in coalbed methane emission reduction: Methane oxidation efficiency and coal wettability
Yinbo Zhou, Ruilin Zhang, Kunyun Tian, Shuang Zhao, Hanfeng Shi, Weidong Gong, Qing Lei Fuel.2023; 349: 128596. CrossRef - Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies
Weronika Goraj, Anna Pytlak, Jarosław Grządziel, Anna Gałązka, Zofia Stępniewska, Anna Szafranek-Nakonieczna Biology.2023; 12(11): 1420. CrossRef - Construction of synthetic anti-fouling consortia: fouling control effects and polysaccharide degradation mechanisms
Ji Qi, Qicheng Zhou, Danlei Huang, Zhong Yu, Fangang Meng Microbial Cell Factories.2023;[Epub] CrossRef - Enzymatic biomethanol production: Future perspective
Saif Khan, Gourav Jain, Alka Srivastava, Praveen C. Verma, Veena Pande, Rama S. Dubey, Mahvish Khan, Shafiul Haque, Saheem Ahmad Sustainable Materials and Technologies.2023; 38: e00729. CrossRef - Isolation of Methane Enriched Bacterial Communities and Application as Wheat Biofertilizer under Drought Conditions: An Environmental Contribution
Adoración Barros-Rodríguez, Carlos García-Gálvez, Pamela Pacheco, Marina G. Kalyuzhnaya, Maximino Manzanera Plants.2023; 12(13): 2487. CrossRef - An Overview on Methanotrophs and the Role of Methylosinus trichosporium OB3b for Biotechnological Applications
Rajendra Singh, Jaewon Ryu, Si Wouk Kim Biotechnology and Bioprocess Engineering.2022; 27(4): 468. CrossRef - Methanol Production using Free and Immobilized Cells of a Newly Isolated Methylosinus trichosporium M19-4
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Rajesh K. Srivastava, Prakash Kumar Sarangi, Latika Bhatia, Akhilesh Kumar Singh, Krushna Prasad Shadangi Biomass Conversion and Biorefinery.2022; 12(5): 1851. CrossRef - Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O2-Availability
Amber Yasemin Shirin de Zoete, Hendrik Gideon Brink, Joshua Cornelus Beukes, Ignatius Leopoldus van Rooyen, Willie Nicol Processes.2021; 9(11): 2039. CrossRef - Plant Growth Stimulation by Microbial Consortia
Gustavo Santoyo, Paulina Guzmán-Guzmán, Fannie Isela Parra-Cota, Sergio de los Santos-Villalobos, Ma. del Carmen Orozco-Mosqueda, Bernard R. Glick Agronomy.2021; 11(2): 219. CrossRef - Recent trends in methane to bioproduct conversion by methanotrophs
Aleksandra Gęsicka, Piotr Oleskowicz-Popiel, Mateusz Łężyk Biotechnology Advances.2021; 53: 107861. CrossRef - Modelling and multi-objective optimization for simulation of hydrogen production using a photosynthetic consortium
Dulce J. Hernández-Melchor, Beni Camacho-Pérez, Elvira Ríos-Leal, Jesus Alarcón-Bonilla, Pablo A. López-Pérez International Journal of Chemical Reactor Engineering.2020;[Epub] CrossRef - Environmental and Microbial Interactions Shape Methane-Oxidizing Bacterial Communities in a Stratified Lake
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- Enhanced method for microbial community DNA extraction and purification from agricultural yellow loess soil
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Mathur Nadarajan Kathiravan , Geun Ho Gim , Jaewon Ryu , Pyung Il Kim , Chul Won Lee , Si Wouk Kim
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J. Microbiol. 2015;53(11):767-775. Published online October 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-5454-0
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980
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Abstract
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In this study, novel DNA extraction and purification methods
were developed to obtain high-quantity and reliable quality
DNA from the microbial community of agricultural yellow
loess soil samples. The efficiencies of five different soil DNAextraction
protocols were evaluated on the basis of DNA
yield, quality and DNA shearing. Our suggested extraction
method , which used CTAB, EDTA and cell membrane lytic
enzymes in the extraction followed by DNA precipitation
using isopropanol, yielded a maximum DNA content of 42.28
± 5.59 μg/g soil. In addition, among the five different purification
protocols, the acid-treated polyvinyl polypyrrolidone
(PVPP) spin column purification method yielded high-quality
DNA and recovered 91% of DNA from the crude DNA.
Spectrophotometry revealed that the ultraviolet A260/A230
and A260/A280 absorbance ratios of the purified DNA were
1.82 ± 0.03 and 1.94 ± 0.05, respectively. PCR-based 16S rRNA
amplification showed clear bands at ~1.5 kb with acid-treated
PVPP–purified DNA templates. In conclusion, our suggested
extraction and purification protocols can be used to recover
high concentration, high purity, and high-molecular-weight
DNA from clay and silica-rich agricultural soil samples.
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Citations
Citations to this article as recorded by 
- DNA of the three elements: development of molecular genetic techniques in environmental research
Svetlana Galkina Priroda.2025; (2(1314)): 3. CrossRef - Inhibitory Effect of Volatiles from Flavobacterium johnsoniae TR-18 on Aspergillus flavus Growth and Aflatoxin Production
Andong Gong, Jingrong Liu, Gaozhan Wang, Mengge Song, Jianhua Wang, Jingbo Zhang Plant Disease.2025; 109(2): 471. CrossRef - Better Performance of Organic Fertilizer on Improving Yield and Reducing Nitrogen Losses in a Paddy Field as Compared to Biochar-Based Fertilizer
Ke Wang, Shanshan Ying Water, Air, & Soil Pollution.2025;[Epub] CrossRef - TACKLING THE SOIL MICROBIOME – CHALLENGES AND OPORTUNITIES
Andreea-Mihaela Mlesnita Journal of Experimental and Molecular Biology.2024;[Epub] CrossRef - Effects of sodium sulfide application on the growth of Robinia pseudoacacia, heavy metal immobilization, and soil microbial activity in Pb–Zn polluted soil
Xiangyu Zhang, Xiao Lou, Haoqiang Zhang, Wei Ren, Ming Tang Ecotoxicology and Environmental Safety.2020; 197: 110563. CrossRef - Biases from different DNA extraction methods in intestine microbiome research based on 16S rDNA sequencing: a case in the koi carp, Cyprinus carpio var. Koi
Zhuoran Han, Jingfeng Sun, Aijun Lv, Anli Wang MicrobiologyOpen.2019;[Epub] CrossRef - Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
Laynet Angerlyn Puentes , Yusibeska Ramos, Ysvic Inojosa, César Rivera, Angela De Sisto BIO-PROTOCOL.2019;[Epub] CrossRef - Impact of DNA extraction methods on the observed microbial communities from the intestinal flora of the penaeid shrimp Litopenaeus vannamei
Boyun Jiang, Jingfeng Sun, Aijun Lv, Xiucai Hu, Hongyue Shi, YeongYik Sung, Qingkui Wang, Yang Wang FEMS Microbiology Letters.2019;[Epub] CrossRef - Illumina sequencing and assessment of new cost-efficient protocol for metagenomic-DNA extraction from environmental water samples
Mariam Hassan, Tamer Essam, Salwa Megahed Brazilian Journal of Microbiology.2018; 49: 1. CrossRef - A modified method for genomic DNA extraction from the fish intestinal microflora
Zhuoran Han, Jingfeng Sun, Aijun Lv, YeongYik Sung, Xueliang Sun, Hongyue Shi, Xiucai Hu, Anli Wang, Kezhi Xing AMB Express.2018;[Epub] CrossRef - Isolation of nematode DNA from 100 g of soil using Fe3O4 super paramagnetic nanoparticles
Adrienne M. Gorny, Frank S. Hay, Xiaohong Wang, Sarah J. Pethybridge Nematology.2018; 20(3): 271. CrossRef - Evaluation of the Punch-it™ NA-Sample kit for detecting microbial DNA in blood culture bottles using PCR-reverse blot hybridization assay
Jungho Kim, Hye-young Wang, Seoyong Kim, Soon Deok Park, Kwangmin Yu, Hyo Youl Kim, Young Uh, Hyeyoung Lee Journal of Microbiological Methods.2016; 128: 24. CrossRef
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