- Novel nuclear targeting coiled-coil protein of Helicobacter pylori showing Ca2+-independent, Mg2+-dependent DNase I activity
-
Young Chul Kwon , Sinil Kim , Yong Seok Lee , Je Chul Lee , Myung-Je Cho , Woo-Kon Lee , Hyung-Lyun Kang , Jae-Young Song , Seung Chul Baik , Hyeon Su Ro
-
J. Microbiol. 2016;54(5):387-395. Published online April 20, 2016
-
DOI: https://doi.org/10.1007/s12275-016-5631-9
-
-
921
View
-
0
Download
-
7
Crossref
-
 Abstract
 PDF
-
HP0059, an uncharacterized gene of Helicobacter pylori, encodes
a 284-aa-long protein containing a nuclear localization
sequence (NLS) and multiple leucine-rich heptad repeats.
Effects of HP0059 proteins in human stomach cells were assessed
by incubation of recombinant HP0059 proteins with
the AGS human gastric carcinoma cell line. Wild-type HP0059
proteins showed cytotoxicity in AGS cells in a concentrationdependent
manner, whereas NLS mutant protein showed no
effect, suggesting that the cytotoxicity is attributed to host nuclear
localization. AGS cells transfected with pEGFP-HP0059
plasmid showed strong GFP signal merged to the chromosomal
DNA region. The chromosome was fragmented into
multiple distinct dots merged with the GFP signal after 12 h
of incubation. The chromosome fragmentation was further
explored by incubation of AGS chromosomal DNA with recombinant
HP0059 proteins, which leaded to complete degradation
of the chromosomal DNA. HP0059 protein also
degraded circular plasmid DNA without consensus, being an
indication of DNase I activity. The DNase was activated by
MgCl2, but not by CaCl2. The activity was completely blocked
by EDTA. The optimal pH and temperature for DNase activity
were 7.0–8.0 and 55°C, respectively. These results indicate
that HP0059 possesses a novel DNase I activity along
with a role in the genomic instability of human gastric cells,
which may result in the transformation of gastric cells.
-
Citations
Citations to this article as recorded by  - Identification of candidate nucleomodulins in ESKAPE bacteria – in silico prediction of bacterial proteins harboring canonical nuclear localization sequences
Laura Riedl, Filip Vilotic, Luzia Burger, Annika Walter, Juergen Fritsch
Frontiers in Cellular and Infection Microbiology.2026;[Epub] CrossRef - Nucleomodulins from gut bacteria: diverse mechanisms of translocation and interaction with host nuclear processes
Sania Korgaonkar, Chandrani Bose, Swadha Anand, Issac Cann
Applied and Environmental Microbiology.2025;[Epub] CrossRef - The Identification of a Novel Nucleomodulin MbovP467 of Mycoplasmopsis bovis and Its Potential Contribution in Pathogenesis
Abdul Raheem, Doukun Lu, Abdul Karim Khalid, Gang Zhao, Yingjie Fu, Yingyu Chen, Xi Chen, Changmin Hu, Jianguo Chen, Huanchun Chen, Aizhen Guo
Cells.2024; 13(7): 604. CrossRef - Molecular Coevolution of Nuclear and Nucleolar Localization Signals inside the Basic Domain of HIV-1 Tat
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna V. Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia I. Kachalova, Yana R. Musinova, Andrey V. Golovin, Yegor S. Va
Journal of Virology.2022;[Epub] CrossRef - Bacterial nucleomodulins and cancer: An unresolved enigma
Abdul Arif Khan, Zakir Khan
Translational Oncology.2021; 14(1): 100922. CrossRef - TatD DNases of African trypanosomes confer resistance to host neutrophil extracellular traps
Kai Zhang, Ning Jiang, Hongyu Chen, Naiwen Zhang, Xiaoyu Sang, Ying Feng, Ran Chen, Qijun Chen
Science China Life Sciences.2021; 64(4): 621. CrossRef - Origin of the nuclear proteome on the basis of pre-existing nuclear localization signals in prokaryotic proteins
Olga M. Lisitsyna, Margarita A. Kurnaeva, Eugene A. Arifulin, Maria Y. Shubina, Yana R. Musinova, Andrey A. Mironov, Eugene V. Sheval
Biology Direct.2020;[Epub] CrossRef
- An Easy Way for the Rapid Purification of Recombinant Proteins from Helicobacter pylori Using a Newly Designed Expression Vector
-
Hyung-Lyun Kang , Jin-Sung Jo , Soon-Uck Kwon , Jae-Young Song , Ji-Hyun Seo , Myung-Je Cho , Seung-Chul Baik , Hee-Shang Youn , Kwang-Ho Rhee , Woo-Kon Lee
-
J. Microbiol. 2014;52(7):604-608. Published online June 28, 2014
-
DOI: https://doi.org/10.1007/s12275-014-3679-y
-
-
909
View
-
1
Download
-
1
Crossref
-
Abstract
PDF
-
We constructed a H. pylori expression vector which consisted of both a His-tag and a GST tag as purification tools for recombinant protein and a chloramphenicol resistant cat gene as a reporter. The backbone of the vector pBK contained an ColEI origin of replication and a kanamycin resistant gene. A set of oligos for the His-tag and the PCR product of gst (glutathione S-transferase) gene were inserted sequentially in frame in the multi-cloning site of pBK. The orf of cat was inserted downstream of the gst to generate pBKHGC. The 3' part of H. pylori clpB and flaA were cloned into the vector which was introduced into H. pylori. Recombinant proteins were purified by GSH affinity column, digested with thrombin and were analyzed by western blotting. The final recombinant proteins were successfully purified.
-
Citations
Citations to this article as recorded by - Gene polymorphisms of pathogenic Helicobacter pylori in patients with different types of gastrointestinal diseases
Yu-Li Chen, Xiao-Qiang Mo, Gan-Rong Huang, Yan-Qiang Huang, Juan Xiao, Li-Juan Zhao, Hong-Yu Wei, Qian Liang
World Journal of Gastroenterology.2016; 22(44): 9718. CrossRef
- Profiling of the Bacteria Responsible for Pyogenic Liver Abscess by 16S rRNA Gene Pyrosequencing
-
Yun Gyu Song , Sang Gun Shim , Kwang Min Kim , Dong-Hae Lee , Dae-Soo Kim , Sang-Haeng Choi , Jae-Young Song , Hyung-Lyun Kang , Seung-Chul Baik , Woo-Kon Lee , Myung-Je Cho , Kwang-Ho Rhee
-
J. Microbiol. 2014;52(6):504-509. Published online May 29, 2014
-
DOI: https://doi.org/10.1007/s12275-014-4241-7
-
-
981
View
-
1
Download
-
26
Crossref
-
Abstract
PDF
-
Pyogenic liver abscess (PLA) is a severe disease with considerable mortality and is often polymicrobial. Understanding the pathogens that cause PLA is the basis for PLA treatment. Here, we profiled the bacterial composition in PLA fluid by pyrosequencing the 16S ribosomal RNA (rRNA) gene based on next-generation sequencing (NGS) technology to identify etiological agents of PLA and to provide information of their 16S rRNA sequences for application to DNA-based techniques in the hospital. Twenty patients with PLA who underwent percutaneous catheter drainage, abscess culture, and blood culture for isolates were included. Genomic DNAs from abscess fluids were subjected to polymerase chain reaction and pyrosequencing of the 16S rRNA gene with a 454 GS Junior System. The abscess and blood cultures were positive in nine (45%) and four (20%) patients, respectively.
Pyrosequencing of 16S rRNA gene showed that 90% of the PLA fluid samples contained single or multiple genera of known bacteria such as Klebsiella, Fusobacterium, Streptococcus, Bacteroides, Prevotella, Peptostreptococcus, unassigned Enterobacteriaceae, and Dialister. Klebsiella was predominantly
found in the PLA fluid samples. All samples that carried unassigned bacteria had 26.8% reads on average. We demonstrated that the occurrence of PLA was associated with eight known bacterial genera as well as unassigned bacteria and that 16S rRNA gene sequencing was more useful than conventional culture methods for accurate identification of bacterial pathogens from PLA.
-
Citations
Citations to this article as recorded by - A Complicated Multiloculated Pyogenic Liver Abscess With Spontaneous Rupture and Life-Threatening Hemorrhage Successfully Managed With Transarterial Embolization: A Case Report
Md Monirul Azim, Abdul Haleem Abbasi, Ameena Rahman
Cureus.2026;[Epub] CrossRef - Massive Sequencing of V3-V4 Hypervariable Region in Pyogenic Liver Abscesses Reveals the Presence of Unusual Bacteria Not Detected by Classical Culture Methods
Verónica Fernández-Sánchez, Estibeyesbo Said Plascencia-Nieto, Mónica Alethia Cureño-Díaz, Emilio Mariano Durán-Manuel, Aida Verónica Rodríguez-Tovar, Claudia Camelia Calzada-Mendoza, Clemente Cruz-Cruz, Miguel Ángel Loyola-Cruz, María Elizbeth Álvarez-Sá
Microorganisms.2025; 13(1): 131. CrossRef - Pyogenic liver abscess and sepsis caused by mixed anaerobic bacteria in an immunocompetent adult: a case report
Ziwen Fan, Mu Mei, Cen Chen
Frontiers in Medicine.2025;[Epub] CrossRef - Comparative microbiome analysis of contracted breast capsules: a cross-sectional study
Tae Hyun Park, Min-Ji Kim, Yeonkyeong Lee, Joon Seok Lee, Jeeyeon Lee, Ho Yong Park, Jae-Ho Shin, Jung Dug Yang
BMC Microbiology.2025;[Epub] CrossRef - Alterations of Gut Microbiota in Pyogenic Liver Abscess Patients with and without Type 2 Diabetes Mellitus
Shanshan Liu, Xiaomeng Ge, Xizhan Xu, Kang Zhang, Xinxin Lu
Infection and Drug Resistance.2024; Volume 17: 2149. CrossRef - Absceso hepático
G. Rossi, M. Ronot, V. Zarrouk, A. Lefort
EMC - Tratado de Medicina.2024; 28(3): 1. CrossRef - Comprehensive analysis of the microbiome and metabolome in pus from pyogenic liver abscess patients with and without diabetes mellitus
Yawen Guo, Hairui Wang, Zhaoyu Liu, Zhihui Chang
Frontiers in Microbiology.2023;[Epub] CrossRef - Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses
Zhijie Zhang, Hairui Wang, Yawen Guo, Zhaoyu Liu, Zhihui Chang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Involvement of Fusobacterium nucleatum in malignancies except for colorectal cancer: A literature review
Zhixing He, Wei Tian, Qichun Wei, Jing Xu
Frontiers in Immunology.2022;[Epub] CrossRef - Liver abscesses in the Western pediatric population
L Grossar, I Hoffman, E Sokal, X Stéphenne, P Witters
Acta Gastro Enterologica Belgica.2022; 85(3): 439. CrossRef - Characterization of abscesses from liver, pancreas and kidney using deep sequencing of the 16S rRNA gene
Joanna Małgorzata Bivand, Randi Monsen Nygaard, Øyvind Kommedal
Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115277. CrossRef -
Brevundimonas diminuta coinfection as source of pyogenic liver abscess
Jacob Burch, Shilpa Tatineni, Ikponmwosa Enofe, Heather Laird-Fick
BMJ Case Reports.2021; 14(5): e236235. CrossRef - Real‐time PCR versus MALDI‐TOF MS and culture‐based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals
N.K. Abd El‐Aziz, A.A Gharib, E.A.A. Mohamed, A.H. Hussein
Journal of Applied Microbiology.2021; 130(5): 1630. CrossRef - Neonatal Exposure to BPA, BDE-99, and PCB Produces Persistent Changes in Hepatic Transcriptome Associated With Gut Dysbiosis in Adult Mouse Livers
Joe Jongpyo Lim, Moumita Dutta, Joseph L Dempsey, Hans-Joachim Lehmler, James MacDonald, Theo Bammler, Cheryl Walker, Terrance J Kavanagh, Haiwei Gu, Sridhar Mani, Julia Yue Cui
Toxicological Sciences.2021; 184(1): 83. CrossRef - Impact of Qualitative and Quantitative Biliary Contamination Status on the Incidence of Postoperative Infection Complications in Patients Undergoing Pancreatoduodenectomy
Masaya Suenaga, Yukihiro Yokoyama, Tsutomu Fujii, Suguru Yamada, Junpei Yamaguchi, Masamichi Hayashi, Takashi Asahara, Masato Nagino, Yasuhiro Kodera
Annals of Surgical Oncology.2021; 28(1): 560. CrossRef - Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota
Kavita Agarwal, Lloyd S. Robinson, Somya Aggarwal, Lynne R. Foster, Ariel Hernandez-Leyva, Hueylie Lin, Brett A. Tortelli, Valerie P. O’Brien, Liza Miller, Andrew L. Kau, Hilary Reno, Nicole M. Gilbert, Warren G. Lewis, Amanda L. Lewis, Ken Cadwell
PLOS Biology.2020; 18(8): e3000788. CrossRef - Clinical characteristics and treatment outcomes in a cohort of patients with pyogenic and amoebic liver abscess
Lorna Neill, Frances Edwards, Simon M. Collin, David Harrington, Dominic Wakerley, Guduru Gopal Rao, Alastair C. McGregor
BMC Infectious Diseases.2019;[Epub] CrossRef - Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology
Yuya Nogami, Kouji Banno, Masataka Adachi, Haruko Kunitomi, Yusuke Kobayashi, Eiichiro Tominaga, Daisuke Aoki
Infectious Diseases in Obstetrics and Gynecology.2019; 2019: 1. CrossRef - PCR Characterization of Microbiota on Contracted and Non-Contracted Breast Capsules
Yara Bachour, Linda Poort, Stephan P. Verweij, Gijs van Selms, Hay A. H. Winters, Marco J. P. F. Ritt, Frank B. Niessen, Andries E. Budding
Aesthetic Plastic Surgery.2019; 43(4): 918. CrossRef - Detection of bacterial DNA from central venous catheter removed from patients by next generation sequencing: a preliminary clinical study
Ken-ichi Okuda, Yutaka Yoshii, Satomi Yamada, Akio Chiba, Ippei Hironaka, Seiji Hori, Katsuhiko Yanaga, Yoshimitsu Mizunoe
Annals of Clinical Microbiology and Antimicrobials.2018;[Epub] CrossRef - Rapid Communication: 16S ribosomal ribonucleic acid characterization of liver abscesses in feedlot cattle from three states in the United States1
M. D. Weinroth, C. R. Carlson, J. N. Martin, J. L. Metcalf, P. S. Morley, K. E. Belk
Journal of Animal Science.2017; 95(10): 4520. CrossRef - Fusobacterium nucleatum in gastroenterological cancer: Evaluation of measurement methods using quantitative polymerase chain reaction and a literature review
Kensuke Yamamura, Yoshifumi Baba, Keisuke Miyake, Kenichi Nakamura, Hironobu Shigaki, Kosuke Mima, Junji Kurashige, Takatsugu Ishimoto, Masaaki Iwatsuki, Yasuo Sakamoto, Yoichi Yamashita, Naoya Yoshida, Masayuki Watanabe, Hideo Baba
Oncology Letters.2017;[Epub] CrossRef - Fusobacterial liver abscess: a case report and review of the literature
Dilip Jayasimhan, Linus Wu, Paul Huggan
BMC Infectious Diseases.2017;[Epub] CrossRef - Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples
Andries E. Budding, Martine Hoogewerf, Christina M. J. E. Vandenbroucke-Grauls, Paul H. M. Savelkoul, N. A. Ledeboer
Journal of Clinical Microbiology.2016; 54(4): 934. CrossRef - Fusobacterium nucleatum infections: clinical spectrum and bacteriological features of 78 cases
E. Denes, O. Barraud
Infection.2016; 44(4): 475. CrossRef - Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines
Edward Alain B. Pajarillo, Jong Pyo Chae, Marilen P. Balolong, Hyeun Bum Kim, Kang-Seok Seo, Dae-Kyung Kang
Journal of Microbiology.2014; 52(8): 646. CrossRef
- Genetic Organization and Conjugal Plasmid DNA Transfer of pHP69, a Plasmid from a Korean Isolate of Helicobacter pylori
-
Jung-Soo Joo , Jae-Young Song , Seung-Chul Baik , Woo-Kon Lee , Myung-Je Cho , Kon-Ho Lee , Hee-Shang Youn , Ji-Hyun Seo , Kwang-Ho Rhee , Hyung-Lyun Kang
-
J. Microbiol. 2013;51(6):889-889.
-
DOI: https://doi.org/10.1007/s12275-013-0727-y
-
-
Abstract
PDF
-
In the article by Joo et al. published in the Journal of Microbiology 2012; 50, 955-961. Dr. YoungAh Cho, one of coauthor’s
name was inadvertently missing. Complete authors information is as follow.
Jung-Soo Joo4, Jae-Young Song1,3, Seung-Chul Baik1,3, Woo-Kon Lee1,3, Myung-Je Cho1,3, Kon-Ho Lee1,3,
YoungAh Cho1, Hee-Shang Youn2, Ji-Hyun Seo2, Kwang-Ho Rhee1,3, and Hyung-Lyun Kang1,3*
- Genetic Organization and Conjugal Plasmid DNA Transfer of pHP69, a Plasmid from a Korean Isolate of Helicobacter pylori
-
Jung-Soo Joo , Jae-Young Song , Seung-Chul Baik , Woo-Kon Lee , Myung-Je Cho , Kon-Ho Lee , Hee-Shang Youn , Ji-Hyun Seo , Kwang-Ho Rhee , Hyung-Lyun Kang
-
J. Microbiol. 2012;50(6):955-961. Published online December 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2580-9
-
-
819
View
-
0
Download
-
5
Crossref
-
Abstract
PDF
-
We isolated pHP69, a 9,153 bp plasmid from Helicobacter pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences. The nucleic acid and predicted amino acid sequences of these ORFs exhibited significant homology to those of other H. pylori plasmids. pHP69 repA encodes a replication initiation protein and its amino acid sequence is similar to those of replicase proteins from theta-type plasmids. pHP69 contains two types of repeat
sequences (R1 and R2), a MOBHEN family mobilization region comprising mobC, mobA, mobB, and mobD, and genes encoding microcin B and C. Among the 36 H. pylori strains containing plasmids, mobA or mccBC are present in 12 or 6, respectively and 3 contain both genes. To examine intrinsic capability of H. pylori for conjugative plasmid transfer, a shuttle vector pBHP69KH containing pHP69 and replication origin of pBR322 was constructed. It was shown that this vector could stably replicate and be mobilized among clinical H. pylori strains and demonstrated to gene transfer by natural plasmid.
-
Citations
Citations to this article as recorded by - Construction and characterization of the first Arcobacter butzleri - Escherichia coli shuttle vector
Adrián Salazar-Sánchez, Rodrigo Alonso, Aurora Fernández-Astorga, Ilargi Martínez-Ballesteros, Irati Martinez-Malaxetxebarria
Archives of Microbiology.2026;[Epub] CrossRef - Comprehensive plasmidomic analysis of Helicobacter pylori reveals the potential role of plasmids in pathogenic adaptation and a novel putative toxin-antitoxin system
Bradd Mendoza-Guido, Juan D Romero-Carpio, Silvia Molina-Castro
FEMS Microbiology Letters.2025;[Epub] CrossRef - Four Chromosomal Type IV Secretion Systems in Helicobacter pylori: Composition, Structure and Function
Wolfgang Fischer, Nicole Tegtmeyer, Kerstin Stingl, Steffen Backert
Frontiers in Microbiology.2020;[Epub] CrossRef - Two novel transcriptional reporter systems for monitoring Helicobacter pylori stress responses
A.M. Belova, D.V. Basmanov, V.V. Babenko, O.V. Podgorny, T.V. Mitko, K.A. Prusakov, D.V. Klinov, V.N. Lazarev
Plasmid.2019; 106: 102442. CrossRef - DNA transfer in the gastric pathogen Helicobacter pylori
Esther Fernandez-Gonzalez, Steffen Backert
Journal of Gastroenterology.2014; 49(4): 594. CrossRef
- Packaging of Porcine Reproductive and Respiratory Syndrome Virus Replicon RNA by a Stable Cell Line Expressing Its Nucleocapsid Protein
-
Byung-Hak Song , Jeong-Min Kim , Jin-Kyoung Kim , Han-Saem Jang , Gil-Nam Yun , Eun-Jin Choi , Jae-Young Song , Sang-Im Yun , Young-Min Lee
-
J. Microbiol. 2011;49(3):516-523. Published online June 30, 2011
-
DOI: https://doi.org/10.1007/s12275-011-1280-1
-
-
787
View
-
2
Download
-
5
Crossref
-
Abstract
PDF
-
Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the Arteriviridae family, is one of the most common and economically important swine pathogens. Although both live-attenuated and killed-inactivated vaccines against the virus have been available for a decade, PRRSV is still a major problem in the swine industry worldwide. To explore the possibility of producing single-round infectious PRRSV replicon particles as a potential vaccine strategy, we have now generated two necessary components: 1) a stable cell line (BHK/Sinrep19/PRRSV-N) that constitutively expresses the viral nucleocapsid (N) protein localized to the cytoplasm and the nucleolus and 2) a PRRSV replicon vector (pBAC/PRRSV/Replicon-ΔN) with a 177-nucleotide deletion, removing the 3′-half portion of ORF7 in the viral genome, from which the self-replicating propagation-defective replicon RNAs were synthesized in vitro by SP6 polymerase run-off
transcription. Transfection of this replicon RNA into N protein-expressing BHK-21 cells led to the secretion of infectious particles that packaged the replicon RNA, albeit with a low production efficiency of 0.4×102 to 1.1×102 infectious units/ml; the produced particles had only single-round infectivity with no cell-to-cell spread. This trans-complementation system for PRRSV provides a useful platform for studies to define the packaging signals and motifs present within the viral genome and N protein, respectively, and to develop viral replicon-based antiviral vaccines that will stop the infection and spread of this pathogen.
-
Citations
Citations to this article as recorded by - Research progress on the N protein of porcine reproductive and respiratory syndrome virus
Yajie Zheng, Gan Li, Qin Luo, Huiyang Sha, Hang Zhang, Ruining Wang, Weili Kong, Jiedan Liao, Mengmeng Zhao
Frontiers in Microbiology.2024;[Epub] CrossRef - Glycosylated protein 4-deficient PRRSV in complementing cell line shows low virus titer
Hao Guo, Wudong Gaowa, Hongzhe Zhao, Chunyu Liu, Lina Hou, Yongjun Wen, Fengxue Wang
Research in Veterinary Science.2023; 158: 84. CrossRef - Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications
Jayeshbhai Chaudhari, Hiep L. X. Vu
Viruses.2020; 12(11): 1245. CrossRef - Veterinary Replicon Vaccines
Mia C. Hikke, Gorben P. Pijlman
Annual Review of Animal Biosciences.2017; 5(1): 89. CrossRef - Resolution of the cellular proteome of the nucleocapsid protein from a highly pathogenic isolate of porcine reproductive and respiratory syndrome virus identifies PARP-1 as a cellular target whose interaction is critical for virus biology
Long Liu, Zoe Lear, David J. Hughes, Weining Wu, En-min Zhou, Adrian Whitehouse, Hongying Chen, Julian A. Hiscox
Veterinary Microbiology.2015; 176(1-2): 109. CrossRef
|
MSK
